semi_solid_autotrophic_growth_medium_for_gammaproteobacteria

Overview

External ontology

jcm.grmd:1431 (source link unavailable) — SEMI-SOLID AUTOTROPHIC GROWTH MEDIUM FOR GAMMAPROTEOBACTERIA

Applications

• Microbial cultivation

pH

7.0

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1431

Composition

flowchart LR
    CultureMech_015866["semi_solid_autotrophic_growth_medium_for_gammaproteobacteria"]
    ing0_NaCl["NaCl"]
    CultureMech_015866 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_6H2O["MgCl2·6H2O"]
    CultureMech_015866 --> ing1_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_6H2O -.-> CHEBI_86345
    ing2_Na2SO4["Na2SO4"]
    CultureMech_015866 --> ing2_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing2_Na2SO4 -.-> CHEBI_32149
    ing3_KCl["KCl"]
    CultureMech_015866 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_015866 --> ing4_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_2H2O -.-> CHEBI_86158
    ing5_NH4Cl["NH4Cl"]
    CultureMech_015866 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_NaHCO3["NaHCO3"]
    CultureMech_015866 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_Modified_Wolfe_s_mineral_solution__see_Medium_No__915__["Modified Wolfe’s mineral solution (see Medium No. 915 )"]
    CultureMech_015866 --> ing7_Modified_Wolfe_s_mineral_solution__see_Medium_No__915__
    ing8_Resazurin["Resazurin"]
    CultureMech_015866 --> ing8_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Resazurin -.-> CHEBI_8806
    ing9_Distilled_water["Distilled water"]
    CultureMech_015866 --> ing9_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing9_Distilled_water -.-> CHEBI_15377
    ing10_Agarose["Agarose"]
    CultureMech_015866 --> ing10_Agarose
    CHEBI_2511["CHEBI:2511"]
    ing10_Agarose -.-> CHEBI_2511
    ing11_0_5_M_Potassium_phosphate_buffer__pH_7_0_["0.5 M Potassium phosphate buffer (pH 7.0)"]
    CultureMech_015866 --> ing11_0_5_M_Potassium_phosphate_buffer__pH_7_0_
    ing12_Vitamin_solution___see_Medium_No__915__["Vitamin solution* (see Medium No. 915 )"]
    CultureMech_015866 --> ing12_Vitamin_solution___see_Medium_No__915__
    ing13_0_01_M_FeSO4_7H2O_solution__in_0_02_N_HCl_["0.01 M FeSO4·7H2O solution (in 0.02 N HCl)"]
    CultureMech_015866 --> ing13_0_01_M_FeSO4_7H2O_solution__in_0_02_N_HCl_
    ing14_40__Na2S2O3_5H2O_solution["40% Na2S2O3·5H2O solution"]
    CultureMech_015866 --> ing14_40__Na2S2O3_5H2O_solution
    ing15_5__L_Cysteine_HCl_H2O_solution["5% L-Cysteine·HCl·H2O solution"]
    CultureMech_015866 --> ing15_5__L_Cysteine_HCl_H2O_solution

Ingredients (16)

Preparation

1. Mix components thoroughly, except agarose, and adjust pH to 7.0.
2. Add agarose to the medium and autoclave under a N2 gas atmosphere.
3. After cooling to 50℃, add the following solutions to the medium aseptically and anaerobically (autoclaved or *filter-sterilized).
4. Keep the medium temperature at 50℃ until the medium is distributed into sterile screw-capped test tubes as described below: Adjust pH to 7.0 with sterile HCl or NaOH solution.
5. Dispense the medium to sterile screw-capped test tubes (typically 11 ml medium into sterile Hungate tubes) under air and cap.
6. Allow to stand until the medium is solidified and a O2-gradient formed (as evidenced by a pink upper oxic zone and a colorless lower anoxic zone).
7. Inoculate cells just below the pink-colorless interface in the medium tube using a syringe.
8. Comment: The medium tubes can be stocked under a N2 gas atmosphere.
9. In this case, prior to use, fill the head space with air and allow to stand until the O2 gradient is formed.

Target organisms

No target organisms are recorded.

Provenance