limnochordia_ln_medium

CultureMech:015867 bacterial COMPLEX LIQUID
Original name: LIMNOCHORDIA LN MEDIUM

Overview

External ontology
jcm.grmd:1435 (source link unavailable) — LIMNOCHORDIA LN MEDIUM
Applications
  • Microbial cultivation
pH
6.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1435

Composition

flowchart LR
    CultureMech_015867["limnochordia_ln_medium"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_015867 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_KH2PO4["KH2PO4"]
    CultureMech_015867 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_EDTA_2Na["EDTA•2Na"]
    CultureMech_015867 --> ing2_EDTA_2Na
    ing3_FeSO4_7H2O["FeSO4·7H2O"]
    CultureMech_015867 --> ing3_FeSO4_7H2O
    ing4_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_015867 --> ing4_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_7H2O -.-> CHEBI_31795
    ing5_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_015867 --> ing5_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_2H2O -.-> CHEBI_86158
    ing6_NaCl["NaCl"]
    CultureMech_015867 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_Trace_element_solution__see_below_["Trace element solution (see below)"]
    CultureMech_015867 --> ing7_Trace_element_solution__see_below_
    ing8_Resazurin["Resazurin"]
    CultureMech_015867 --> ing8_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Resazurin -.-> CHEBI_8806
    ing9_Distilled_water["Distilled water"]
    CultureMech_015867 --> ing9_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing9_Distilled_water -.-> CHEBI_15377
    ing10_8__NaHCO3_solution_["8% NaHCO3 solution*"]
    CultureMech_015867 --> ing10_8__NaHCO3_solution_
    ing11_10__Glucose_solution["10% Glucose solution"]
    CultureMech_015867 --> ing11_10__Glucose_solution
    ing12_10__Yeast_extract__Oxoid__solution["10% Yeast extract (Oxoid) solution"]
    CultureMech_015867 --> ing12_10__Yeast_extract__Oxoid__solution
    ing13_1_0_M_Sodium_thiosulfate_solution["1.0 M Sodium thiosulfate solution"]
    CultureMech_015867 --> ing13_1_0_M_Sodium_thiosulfate_solution
    ing14_Trace_vitamins___see_Medium_No__197__["Trace vitamins* (see Medium No. 197 )"]
    CultureMech_015867 --> ing14_Trace_vitamins___see_Medium_No__197__
    ing15_5__L_Cysteine_HCl_H2O_solution["5% L-Cysteine·HCl·H2O solution"]
    CultureMech_015867 --> ing15_5__L_Cysteine_HCl_H2O_solution
    ing16_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_015867 --> ing16_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing16_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing17_FeCl2_4H2O["FeCl2·4H2O"]
    CultureMech_015867 --> ing17_FeCl2_4H2O
    CHEBI_86249["CHEBI:86249"]
    ing17_FeCl2_4H2O -.-> CHEBI_86249
    ing18_MnCl2_4H2O["MnCl2·4H2O"]
    CultureMech_015867 --> ing18_MnCl2_4H2O
    ing19_CoCl2_6H2O["CoCl2·6H2O"]
    CultureMech_015867 --> ing19_CoCl2_6H2O
    ing20_ZnCl2["ZnCl2"]
    CultureMech_015867 --> ing20_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing20_ZnCl2 -.-> CHEBI_49976
    ing21_CuCl2_2H2O["CuCl2·2H2O"]
    CultureMech_015867 --> ing21_CuCl2_2H2O
    CHEBI_86318["CHEBI:86318"]
    ing21_CuCl2_2H2O -.-> CHEBI_86318
    ing22_H3BO3["H3BO3"]
    CultureMech_015867 --> ing22_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing22_H3BO3 -.-> CHEBI_33118
    ing23_Na2MoO4_2H2O["Na2MoO4·2H2O"]
    CultureMech_015867 --> ing23_Na2MoO4_2H2O
    CHEBI_75213["CHEBI:75213"]
    ing23_Na2MoO4_2H2O -.-> CHEBI_75213
    ing24_NiCl2_6H2O["NiCl2·6H2O"]
    CultureMech_015867 --> ing24_NiCl2_6H2O
    CHEBI_34887["CHEBI:34887"]
    ing24_NiCl2_6H2O -.-> CHEBI_34887
    ing25_NaCl["NaCl"]
    CultureMech_015867 --> ing25_NaCl
    ing25_NaCl -.-> CHEBI_26710
    ing26_Na2SeO3["Na2SeO3"]
    CultureMech_015867 --> ing26_Na2SeO3
    CHEBI_48843["CHEBI:48843"]
    ing26_Na2SeO3 -.-> CHEBI_48843
    ing27_Na2WO4_2H2O["Na2WO4·2H2O"]
    CultureMech_015867 --> ing27_Na2WO4_2H2O
    CHEBI_63939["CHEBI:63939"]
    ing27_Na2WO4_2H2O -.-> CHEBI_63939
    ing28_Distilled_water["Distilled water"]
    CultureMech_015867 --> ing28_Distilled_water
    ing28_Distilled_water -.-> CHEBI_15377

Ingredients (29)

# Ingredient Concentration Ontology Notes
1 K2HPO4
HO4P.2K 174.174 g/mol
0.78 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.7 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 EDTA•2Na 0.04 G_PER_L
4 FeSO4·7H2O 0.01 G_PER_L
5 MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
0.25 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
6 CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 NaCl
Cl.Na 58.443 g/mol
0.2 G_PER_L CHEBI:26710
sodium chloride
8 Trace element solution (see below) 10.0 ML_PER_L
9 Resazurin
C12H7NO4 229.191 g/mol
0.5 MG_PER_L CHEBI:8806
Resazurin
10 Distilled water
H2O 18.015 g/mol
940.0 ML_PER_L CHEBI:15377
water
11 8% NaHCO3 solution* 25.0 ML_PER_L
12 10% Glucose solution 5.0 ML_PER_L
13 10% Yeast extract (Oxoid) solution 5.0 ML_PER_L
14 1.0 M Sodium thiosulfate solution 5.0 ML_PER_L
15 Trace vitamins* (see Medium No. 197 ) 10.0 ML_PER_L
16 5% L-Cysteine·HCl·H2O solution 5.0 ML_PER_L
17 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
12.8 G_PER_L CHEBI:44557
nitrilotriacetic acid
18 FeCl2·4H2O
Cl2Fe.4H2O 198.811 g/mol
1.0 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
19 MnCl2·4H2O 0.1 G_PER_L
20 CoCl2·6H2O 0.03 G_PER_L
21 ZnCl2
2Cl.Zn 136.296 g/mol
0.1 G_PER_L CHEBI:49976
zinc dichloride
22 CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
0.02 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
23 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
24 Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate
25 NiCl2·6H2O
Cl2Ni 129.599 g/mol
0.1 G_PER_L CHEBI:34887
nickel dichloride
26 NaCl
Cl.Na 58.443 g/mol
1.0 G_PER_L CHEBI:26710
sodium chloride
27 Na2SeO3
2Na.O3Se 172.937 g/mol
0.02 G_PER_L CHEBI:48843
disodium selenite
28 Na2WO4·2H2O
2H2O.2Na.O4W 329.846 g/mol
0.04 G_PER_L CHEBI:63939
sodium tungstate dihydrate
29 Distilled water
H2O 18.015 g/mol
1.0 ML_PER_L CHEBI:15377
water

Preparation

  1. Mix components thoroughly, sparge the medium with a N2-CO2 (80:20, v/v) gas mixture and autoclave under the same gas mixture.
  2. After cooling, aseptically and anaerobically add the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized): Prior to use, add per liter the following solution (autoclaved and stored under a N2 atmosphere): Adjust pH to 6.5, if necessary.
  3. Trace element solution: Dissolve nitrilotriacetic acid first in 200 ml of distilled water, and adjust pH 6.5 with KOH.
  4. Then, add the remaining salts and adjust volume to 1000 ml.
  5. Readjust pH to 6.5.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-06-02T07:09:17.927951+00:00 jcm-grmd-import Scraped from JCM GRMD database Direct import from JCM GRMD=1435. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps.
2026-06-03T02:00:03.396852Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-06-03T07:12:22.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-08-15T20:07:34.164621+00:00 dedupe_identical_ingredients.py REMOVED_DUPLICATE_INGREDIENT_ROWS Removed 1 ingredient row(s) that were identical in every field to another row in this record, which made the recipe read as double-strength in those components (#263): CaCl2·2H2O.