desulfosporosinus_sb140_medium

CultureMech:015868 bacterial COMPLEX LIQUID
Original name: DESULFOSPOROSINUS SB140 MEDIUM

Overview

External ontology
jcm.grmd:1444 (source link unavailable) — DESULFOSPOROSINUS SB140 MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1444

Composition

flowchart LR
    CultureMech_015868["desulfosporosinus_sb140_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_015868 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_KH2PO4["KH2PO4"]
    CultureMech_015868 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_MgSO4_7H2O["MgSO4-7H2O"]
    CultureMech_015868 --> ing2_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_7H2O -.-> CHEBI_31795
    ing3_KCl["KCl"]
    CultureMech_015868 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_Yeast_extract["Yeast extract"]
    CultureMech_015868 --> ing4_Yeast_extract
    ing5_Ca_NO3_2_4H2O["Ca(NO3)2•4H2O"]
    CultureMech_015868 --> ing5_Ca_NO3_2_4H2O
    ing6_Na2SO4["Na2SO4"]
    CultureMech_015868 --> ing6_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing6_Na2SO4 -.-> CHEBI_32149
    ing7_Trace_element_solution__see_Medium_No__439__["Trace element solution (see Medium No. 439 )"]
    CultureMech_015868 --> ing7_Trace_element_solution__see_Medium_No__439__
    ing8_L_Cysteine_HCl_H2O["L-Cysteine•HCl•H2O"]
    CultureMech_015868 --> ing8_L_Cysteine_HCl_H2O
    CHEBI_91248["CHEBI:91248"]
    ing8_L_Cysteine_HCl_H2O -.-> CHEBI_91248
    ing9_Resazurin["Resazurin"]
    CultureMech_015868 --> ing9_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing9_Resazurin -.-> CHEBI_8806
    ing10_Distilled_water["Distilled water"]
    CultureMech_015868 --> ing10_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing10_Distilled_water -.-> CHEBI_15377
    ing11_5__Na2HPO4_solution["5% Na2HPO4 solution"]
    CultureMech_015868 --> ing11_5__Na2HPO4_solution
    ing12_5__KH2PO4_solution["5% KH2PO4 solution"]
    CultureMech_015868 --> ing12_5__KH2PO4_solution
    ing13_1M_Glycerin_solution["1M Glycerin solution"]
    CultureMech_015868 --> ing13_1M_Glycerin_solution
    ing14_Trace_vitamins___see_Medium_No__197__["Trace vitamins* (see Medium No. 197 )"]
    CultureMech_015868 --> ing14_Trace_vitamins___see_Medium_No__197__

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.45 G_PER_L CHEBI:62946
ammonium sulfate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.05 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 MgSO4-7H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 KCl
Cl.K 74.551 g/mol
0.05 G_PER_L CHEBI:32588
potassium chloride
5 Yeast extract 0.5 G_PER_L FOODON:03315426
Yeast extract
6 Ca(NO3)2•4H2O 0.014 G_PER_L
7 Na2SO4
2Na.O4S 142.043 g/mol
3.0 G_PER_L CHEBI:32149
sodium sulfate
8 Trace element solution (see Medium No. 439 ) 1.0 ML_PER_L
9 L-Cysteine•HCl•H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
10 Resazurin
C12H7NO4 229.191 g/mol
1.0 MG_PER_L CHEBI:8806
Resazurin
11 Distilled water
H2O 18.015 g/mol
925.0 ML_PER_L CHEBI:15377
water
12 5% Na2HPO4 solution 40.0 ML_PER_L
13 5% KH2PO4 solution 20.0 ML_PER_L
14 1M Glycerin solution 5.0 ML_PER_L
15 Trace vitamins* (see Medium No. 197 ) 10.0 ML_PER_L

Preparation

  1. Mix components thoroughly, adjust pH to 7.0, and autoclave under a N2-CO2 (4:1, v/v) gas mixture.
  2. After cooling, aseptically and anaerobically add the following solutions from anaerobic stocks (autoclaved or *filter-sterilized).

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-06-02T07:09:19.565227+00:00 jcm-grmd-import Scraped from JCM GRMD database Direct import from JCM GRMD=1444. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps.
2026-06-03T02:00:03.481482Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-06-03T07:12:22.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-09-13T00:00:00-07:00 repair_jcm_score10_exact_terms.py GROUNDED_JCM_SCORE10_EXACT_TERMS Applied local mappings for MgSO4-7H2O, Yeast extract, L-Cysteine•HCl•H2O.