pmx_108_medium

CultureMech:015869 bacterial COMPLEX LIQUID
Original name: PMX.108 MEDIUM

Overview

External ontology
jcm.grmd:1445 (source link unavailable) — PMX.108 MEDIUM
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1445

Composition

flowchart LR
    CultureMech_015869["pmx_108_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_015869 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_KH2PO4["KH2PO4"]
    CultureMech_015869 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_MgCl2_x_6H2O["MgCl2 x 6H2O"]
    CultureMech_015869 --> ing2_MgCl2_x_6H2O
    ing3_CaCl2_x_2H2O["CaCl2 x 2H2O"]
    CultureMech_015869 --> ing3_CaCl2_x_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2H2O -.-> CHEBI_86158
    ing4_Sodium_acetate_x_3H2O["Sodium acetate x 3H2O"]
    CultureMech_015869 --> ing4_Sodium_acetate_x_3H2O
    CHEBI_32138["CHEBI:32138"]
    ing4_Sodium_acetate_x_3H2O -.-> CHEBI_32138
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_015869 --> ing5_Yeast_extract
    ing6_Casamino_acids__BD_Difico_["Casamino acids (BD-Difico)"]
    CultureMech_015869 --> ing6_Casamino_acids__BD_Difico_
    ing7_Tryptone__BD_Difico_["Tryptone (BD-Difico)"]
    CultureMech_015869 --> ing7_Tryptone__BD_Difico_
    ing8_Resazurin["Resazurin"]
    CultureMech_015869 --> ing8_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing8_Resazurin -.-> CHEBI_8806
    ing9_Distilled_water["Distilled water"]
    CultureMech_015869 --> ing9_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing9_Distilled_water -.-> CHEBI_15377

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
0.54 G_PER_L CHEBI:31206
ammonium chloride
 JCM Medium 1445 lists 0.54 g NH4Cl.
2 KH2PO4
H2O4P.K 136.084 g/mol
0.14 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 JCM Medium 1445 lists 0.14 g KH2PO4.
3 MgCl2 x 6H2O 0.2 G_PER_L JCM Medium 1445 lists 0.2 g MgCl2 x 6H2O.
4 CaCl2 x 2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.15 G_PER_L CHEBI:86158
calcium chloride dihydrate
 JCM Medium 1445 lists 0.15 g CaCl2 x 2H2O.
5 Sodium acetate x 3H2O
C2H3O2.3H2O.Na 136.079 g/mol
1.36 G_PER_L CHEBI:32138
sodium acetate trihydrate
 JCM Medium 1445 lists 1.36 g sodium acetate trihydrate.
6 Yeast extract 0.09 G_PER_L JCM Medium 1445 lists 0.09 g yeast extract.
7 Casamino acids (BD-Difico) 0.5 G_PER_L JCM Medium 1445 lists 0.5 g Casamino acids.
8 Tryptone (BD-Difico) 1.5 G_PER_L JCM Medium 1445 lists 1.5 g tryptone.
9 Resazurin
C12H7NO4 229.191 g/mol
1.0 MG_PER_L CHEBI:8806
Resazurin
 JCM Medium 1445 lists 1.0 mg resazurin.
10 Distilled water
H2O 18.015 g/mol
970.0 ML_PER_L CHEBI:15377
water
 JCM Medium 1445 lists 970.0 ml distilled water in the base.

Preparation

  1. Mix NH4Cl, KH2PO4, MgCl2 x 6H2O, CaCl2 x 2H2O, sodium acetate trihydrate, yeast extract, Casamino acids, tryptone, Trace mineral solution, Se/W solution, Trace vitamins solution, resazurin, and distilled water.
  2. Bring the mixed base to a boil and cool it under N2-CO2 (4:1, v/v) gas.
  3. Distribute the medium under the same gas mixture, for example 20 ml medium into 60 ml serum bottles, seal with butyl rubber stoppers, inject 10-20% gas-phase volume of H2, and autoclave.
  4. After cooling, aseptically and anaerobically add 8% NaHCO3, 5% L-Cysteine HCl H2O, and 5% Na2S x 9H2O solutions at the JCM Medium 1445 per-liter ratios.

Solutions

Trace mineral solution (JCM Medium 852)

Dose: 1.0 ML_PER_L

JCM Medium 1445 adds 1.0 ml/L Trace mineral solution from JCM 852.

Se/W solution

Dose: 1.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Na2SeO3 2.0 MG_PER_L JCM Medium 1445 Se/W stock lists 2.0 mg Na2SeO3 per liter.
Na2WO4 x 2H2O 1.0 MG_PER_L JCM Medium 1445 Se/W stock lists 1.0 mg Na2WO4 x 2H2O per liter.
JCM Medium 1445 adds 1.0 ml/L of the Se/W solution listed below it.

Trace vitamins solution (JCM Medium 284)

Dose: 2.0 ML_PER_L

JCM Medium 1445 adds 2.0 ml/L Trace vitamins solution from JCM 284.

8% NaHCO3 solution

Dose: 30.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
NaHCO3 80.0 G_PER_L Solute of the 8% NaHCO3 stock added by JCM Medium 1445.
JCM Medium 1445 adds 30.0 ml/L filter-sterilized 8% NaHCO3 solution.

5% L-Cysteine HCl H2O solution

Dose: 4.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
L-Cysteine HCl H2O 50.0 G_PER_L Solute of the 5% L-Cysteine HCl H2O stock added by JCM Medium 1445.
JCM Medium 1445 adds 4.0 ml/L autoclaved 5% L-Cysteine HCl H2O solution.

5% Na2S x 9H2O solution

Dose: 4.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
Na2S x 9H2O 50.0 G_PER_L Solute of the 5% Na2S x 9H2O stock added by JCM Medium 1445.
JCM Medium 1445 adds 4.0 ml/L autoclaved 5% Na2S x 9H2O solution.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-06-02T07:09:20.624232+00:00 jcm-grmd-import Scraped from JCM GRMD database Direct import from JCM GRMD=1445. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps.
2026-06-03T02:00:03.501087Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-06-03T07:12:22.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-09-11T00:00:00-07:00 repair_jcm_1445_pmx108_score15.py RESOLVED_JCM_1445_PMX108_SCORE15 JCM Medium 1445 base salts, organic supplements, resazurin, and distilled water are direct components; before autoclaving, the base also receives JCM 852 Trace mineral solution, the local Se/W solution, and JCM 284 Trace vitamins solution under N2-CO2 (4:1, v/v). After autoclaving, add the bicarbonate, cysteine, and sulfide stocks.