freshwater_r2a_medium

CultureMech:015874 bacterial COMPLEX LIQUID
Original name: FRESHWATER R2A MEDIUM

Overview

External ontology
jcm.grmd:1468 (source link unavailable) — FRESHWATER R2A MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1468

Composition

flowchart LR
    CultureMech_015874["freshwater_r2a_medium"]
    ing0_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_015874 --> ing0_Yeast_extract__BD_Difco_
    ing1_Proteose_peptone_No__3__BD_Difco_["Proteose peptone No. 3 (BD-Difco)"]
    CultureMech_015874 --> ing1_Proteose_peptone_No__3__BD_Difco_
    ing2_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
    CultureMech_015874 --> ing2_Casamino_acids__BD_Difco_
    ing3_Glucose["Glucose"]
    CultureMech_015874 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_KH2PO4["KH2PO4"]
    CultureMech_015874 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_Soluble_starch["Soluble starch"]
    CultureMech_015874 --> ing5_Soluble_starch
    ing6_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_015874 --> ing6_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing6_Sodium_pyruvate -.-> CHEBI_50144
    ing7_NH4Cl["NH4Cl"]
    CultureMech_015874 --> ing7_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing7_NH4Cl -.-> CHEBI_31206
    ing8_FeCl2_solution__see_Medium_No__187__["FeCl2 solution (see Medium No. 187 )"]
    CultureMech_015874 --> ing8_FeCl2_solution__see_Medium_No__187__
    ing9_Trace_element_solution__see_Medium_No__187__["Trace element solution (see Medium No. 187 )"]
    CultureMech_015874 --> ing9_Trace_element_solution__see_Medium_No__187__
    ing10_Selenite_tungstate_solution__see_Medium_No__431__["Selenite tungstate solution (see Medium No. 431 )"]
    CultureMech_015874 --> ing10_Selenite_tungstate_solution__see_Medium_No__431__
    ing11_Vitamin_solution___see_Medium_No__403__["Vitamin solution* (see Medium No. 403 )"]
    CultureMech_015874 --> ing11_Vitamin_solution___see_Medium_No__403__
    ing12_Thiamine_solution___see_Medium_No__403__["Thiamine solution* (see Medium No. 403 )"]
    CultureMech_015874 --> ing12_Thiamine_solution___see_Medium_No__403__
    ing13_Vitamin_B12_solution___see_Medium_No__403__["Vitamin B12 solution* (see Medium No. 403 )"]
    CultureMech_015874 --> ing13_Vitamin_B12_solution___see_Medium_No__403__
    ing14_Resazurin["Resazurin"]
    CultureMech_015874 --> ing14_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing14_Resazurin -.-> CHEBI_8806
    ing15_Distilled_water["Distilled water"]
    CultureMech_015874 --> ing15_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing15_Distilled_water -.-> CHEBI_15377
    ing16_1_M_NaHCO3_solution["1 M NaHCO3 solution"]
    CultureMech_015874 --> ing16_1_M_NaHCO3_solution
    ing17_1_M_Coenzyme_M_solution["1 M Coenzyme M solution"]
    CultureMech_015874 --> ing17_1_M_Coenzyme_M_solution
    ing18_5__Na2S_9H2O_solution["5% Na2S·9H2O solution"]
    CultureMech_015874 --> ing18_5__Na2S_9H2O_solution

Ingredients (19)

# Ingredient Concentration Ontology Notes
1 Yeast extract (BD-Difco) 0.5 G_PER_L FOODON:03315426
Yeast extract
2 Proteose peptone No. 3 (BD-Difco) 0.5 G_PER_L MICRO:0000180 (source link unavailable)
Proteose Peptone
3 Casamino acids (BD-Difco) 0.5 G_PER_L FOODON:03315719
Casamino acids
4 Glucose
C6H12O6 180.156 g/mol
0.5 G_PER_L CHEBI:17234
glucose
5 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
6 Soluble starch 0.5 G_PER_L
7 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
0.5 G_PER_L CHEBI:50144
sodium pyruvate
8 NH4Cl
Cl.H4N 53.492 g/mol
1.5 G_PER_L CHEBI:31206
ammonium chloride
9 FeCl2 solution (see Medium No. 187 ) 1.0 ML_PER_L
10 Trace element solution (see Medium No. 187 ) 1.0 ML_PER_L
11 Selenite tungstate solution (see Medium No. 431 ) 0.5 ML_PER_L
12 Vitamin solution* (see Medium No. 403 ) 1.0 ML_PER_L
13 Thiamine solution* (see Medium No. 403 ) 1.0 ML_PER_L
14 Vitamin B12 solution* (see Medium No. 403 ) 1.0 ML_PER_L
15 Resazurin
C12H7NO4 229.191 g/mol
0.5 MG_PER_L CHEBI:8806
Resazurin
16 Distilled water
H2O 18.015 g/mol
1.0 ML_PER_L CHEBI:15377
water
17 1 M NaHCO3 solution 5.0 ML_PER_L
18 1 M Coenzyme M solution 0.5 ML_PER_L
19 5% Na2S·9H2O solution 6.0 ML_PER_L

Preparation

  1. Mix components and autoclave under a N2-CO2 (80:20, v/v) gas mixture.
  2. After cooling, aseptically and anaerobically, add the following solutions from anaerobic stocks (autoclaved and stored under a N2 atmosphere): Adjust pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-06-02T07:09:26.498891+00:00 jcm-grmd-import Scraped from JCM GRMD database Direct import from JCM GRMD=1468. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps.
2026-06-03T02:00:03.311069Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-06-03T07:12:22.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-09-13T00:00:00-07:00 repair_jcm_score10_exact_terms.py GROUNDED_JCM_SCORE10_EXACT_TERMS Applied local mappings for Yeast extract (BD-Difco), Proteose peptone No. 3 (BD-Difco), Casamino acids (BD-Difco).