desulfobacter_medium_with_horse_serum

CultureMech:015875 bacterial COMPLEX LIQUID
Original name: DESULFOBACTER MEDIUM WITH HORSE SERUM

Overview

External ontology
jcm.grmd:1475 (source link unavailable) — DESULFOBACTER MEDIUM WITH HORSE SERUM
Applications
  • Microbial cultivation
pH
7.1
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1475

Composition

flowchart LR
    CultureMech_015875["desulfobacter_medium_with_horse_serum"]
    ing0_Na2SO4["Na2SO4"]
    CultureMech_015875 --> ing0_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing0_Na2SO4 -.-> CHEBI_32149
    ing1_KH2PO4["KH2PO4"]
    CultureMech_015875 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_NH4Cl["NH4Cl"]
    CultureMech_015875 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_NaCl["NaCl"]
    CultureMech_015875 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_MgCl2_6H2O["MgCl2·6H2O"]
    CultureMech_015875 --> ing4_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_6H2O -.-> CHEBI_86345
    ing5_KCl["KCl"]
    CultureMech_015875 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_015875 --> ing6_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_2H2O -.-> CHEBI_86158
    ing7_Tryptone__Difco_["Tryptone (Difco)"]
    CultureMech_015875 --> ing7_Tryptone__Difco_
    ing8_FeCl2_solution__see_Medium_No__187__["FeCl2 solution (see Medium No. 187 )"]
    CultureMech_015875 --> ing8_FeCl2_solution__see_Medium_No__187__
    ing9_Trace_element_solution__see_Medium_No__187__["Trace element solution (see Medium No. 187 )"]
    CultureMech_015875 --> ing9_Trace_element_solution__see_Medium_No__187__
    ing10_Resazurin["Resazurin"]
    CultureMech_015875 --> ing10_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing10_Resazurin -.-> CHEBI_8806
    ing11_Distilled_water["Distilled water"]
    CultureMech_015875 --> ing11_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing11_Distilled_water -.-> CHEBI_15377
    ing12_Horse_Serum__Gibco__["Horse Serum (Gibco)*"]
    CultureMech_015875 --> ing12_Horse_Serum__Gibco__
    ing13_Trace_vitamins___see_Medium_No__197__["Trace vitamins* (see Medium No. 197 )"]
    CultureMech_015875 --> ing13_Trace_vitamins___see_Medium_No__197__
    ing14_8__NaHCO3_solution_["8% NaHCO3 solution*"]
    CultureMech_015875 --> ing14_8__NaHCO3_solution_
    ing15_5__Na2S_9H2O_solution["5% Na2S·9H2O solution"]
    CultureMech_015875 --> ing15_5__Na2S_9H2O_solution

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 Na2SO4
2Na.O4S 142.043 g/mol
3.0 G_PER_L CHEBI:32149
sodium sulfate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.2 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.3 G_PER_L CHEBI:31206
ammonium chloride
4 NaCl
Cl.Na 58.443 g/mol
21.0 G_PER_L CHEBI:26710
sodium chloride
5 MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
3.0 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
6 KCl
Cl.K 74.551 g/mol
0.5 G_PER_L CHEBI:32588
potassium chloride
7 CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.15 G_PER_L CHEBI:86158
calcium chloride dihydrate
8 Tryptone (Difco) 10.0 G_PER_L
9 FeCl2 solution (see Medium No. 187 ) 1.0 ML_PER_L
10 Trace element solution (see Medium No. 187 ) 1.0 ML_PER_L
11 Resazurin
C12H7NO4 229.191 g/mol
1.0 MG_PER_L CHEBI:8806
Resazurin
12 Distilled water
H2O 18.015 g/mol
920.0 ML_PER_L CHEBI:15377
water
13 Horse Serum (Gibco)* 10.0 ML_PER_L
14 Trace vitamins* (see Medium No. 197 ) 10.0 ML_PER_L
15 8% NaHCO3 solution* 50.0 ML_PER_L
16 5% Na2S·9H2O solution 8.0 ML_PER_L

Preparation

  1. Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) gas mixture.
  2. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filtersterilized): Adjust pH 7.1-7.4, if necessary.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-06-02T07:09:27.545201+00:00 jcm-grmd-import Scraped from JCM GRMD database Direct import from JCM GRMD=1475. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps.
2026-06-03T02:00:03.409516Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-06-03T07:12:22.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers