Overview
- External ontology
- jcm.grmd:1477 (source link unavailable) — GIBBONS MODIFIED MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1477
Composition
flowchart LR
CultureMech_015876["gibbons_modified_medium"]
ing0_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
CultureMech_015876 --> ing0_Casamino_acids__BD_Difco_
ing1_Trisodium_citrate_2H2O["Trisodium citrate·2H2O"]
CultureMech_015876 --> ing1_Trisodium_citrate_2H2O
CHEBI_53258["CHEBI:53258"]
ing1_Trisodium_citrate_2H2O -.-> CHEBI_53258
ing2_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_015876 --> ing2_Yeast_extract__BD_Difco_
ing3_Bacto_peptone__BD_Difco_["Bacto peptone (BD-Difco)"]
CultureMech_015876 --> ing3_Bacto_peptone__BD_Difco_
ing4_KCl["KCl"]
CultureMech_015876 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_015876 --> ing5_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_7H2O -.-> CHEBI_31795
ing6_NaCl["NaCl"]
CultureMech_015876 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_Agar["Agar"]
CultureMech_015876 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
ing8_Distilled_water["Distilled water"]
CultureMech_015876 --> ing8_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing8_Distilled_water -.-> CHEBI_15377
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casamino acids (BD-Difco) | 10.0 G_PER_L | ||
| 2 |
Trisodium citrate·2H2O
C6H5O7.3Na 258.069 g/mol
|
5.0 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 3 | Yeast extract (BD-Difco) | 10.0 G_PER_L | ||
| 4 | Bacto peptone (BD-Difco) | 5.0 G_PER_L | ||
| 5 |
KCl
Cl.K 74.551 g/mol
|
2.0 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
2.0 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
30.0 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 8 | Agar | 15.0 G_PER_L |
CHEBI:2509
agar
|
|
| 9 |
Distilled water
H2O 18.015 g/mol
|
1.0 ML_PER_L |
CHEBI:15377
water
|
Preparation
- Adjust pH to 7.5.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-02T07:09:28.590849+00:00 | jcm-grmd-import | Scraped from JCM GRMD database | Direct import from JCM GRMD=1477. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps. |
| 2026-06-03T02:00:03.513109Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-06-03T07:12:22.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |