solidesulfovibrio_medium

CultureMech:015877 bacterial DEFINED LIQUID
Original name: SOLIDESULFOVIBRIO MEDIUM

Overview

External ontology
jcm.grmd:1479 (source link unavailable) — SOLIDESULFOVIBRIO MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1479

Composition

flowchart LR
    CultureMech_015877["solidesulfovibrio_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_015877 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NH4Cl["NH4Cl"]
    CultureMech_015877 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_Fe_III__EDTA["Fe(III)·EDTA"]
    CultureMech_015877 --> ing2_Fe_III__EDTA
    CHEBI_30729["CHEBI:30729"]
    ing2_Fe_III__EDTA -.-> CHEBI_30729
    ing3_Modified_Wolfe_s_mineral_solution__see_Medium_No__915__["Modified Wolfe’s mineral solution (see Medium No. 915 )"]
    CultureMech_015877 --> ing3_Modified_Wolfe_s_mineral_solution__see_Medium_No__915__
    ing4_Sodium_fumarate["Sodium fumarate"]
    CultureMech_015877 --> ing4_Sodium_fumarate
    CHEBI_115156["CHEBI:115156"]
    ing4_Sodium_fumarate -.-> CHEBI_115156
    ing5_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_015877 --> ing5_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing5_Sodium_pyruvate -.-> CHEBI_50144
    ing6_Distilled_water["Distilled water"]
    CultureMech_015877 --> ing6_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing6_Distilled_water -.-> CHEBI_15377
    ing7_Vitamin_solution___see_Medium_No__915__["Vitamin solution* (see Medium No. 915 )"]
    CultureMech_015877 --> ing7_Vitamin_solution___see_Medium_No__915__
    ing8_5__L_Cysteine_HCl_H2O_solution__pH_7_0_["5% L-Cysteine·HCl·H2O solution (pH 7.0)"]
    CultureMech_015877 --> ing8_5__L_Cysteine_HCl_H2O_solution__pH_7_0_

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.20 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 NH4Cl
Cl.H4N 53.492 g/mol
0.06 G_PER_L CHEBI:31206
ammonium chloride
3 Fe(III)·EDTA
C10H12N2O8.Fe 344.057 g/mol
1.0 MG_PER_L CHEBI:30729
ethylenediaminetetraacetatoferrate(1-)
4 Modified Wolfe’s mineral solution (see Medium No. 915 ) 4.0 ML_PER_L
5 Sodium fumarate
C4H2O4.2Na 160.036 g/mol
0.58 G_PER_L CHEBI:115156
disodium fumarate
6 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
0.44 G_PER_L CHEBI:50144
sodium pyruvate
7 Distilled water
H2O 18.015 g/mol
985.0 ML_PER_L CHEBI:15377
water
8 Vitamin solution* (see Medium No. 915 ) 10.0 ML_PER_L
9 5% L-Cysteine·HCl·H2O solution (pH 7.0) 1.0 ML_PER_L

Preparation

  1. Mix components thoroughly, adjust pH to 7.0 and autoclave under a N2 gas atmosphere.
  2. After cooling, aseptically and anaerobically add the following solutions from anaerobic stocks (autoclaved or *filter-sterilized): Readjust pH to 7.0, if necessary.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-06-02T07:09:29.642056+00:00 jcm-grmd-import Scraped from JCM GRMD database Direct import from JCM GRMD=1479. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps.
2026-06-03T02:00:03.568293Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-06-03T07:12:22.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers