bold_s_basal_medium_bbm

CultureMech:015878 algae COMPLEX SOLID_AGAR
Original name: Bold's Basal Medium (BBM)

Overview

External ontology
jcm.grmd:1481 (source link unavailable) — Bold's Basal Medium (BBM)
Applications
  • Microbial cultivation
Notes
JCM Medium 1481 lists 20 g agar and 980 ml distilled water for one liter of Bold's Basal Medium (BBM) and instructs adding 20 ml Sigma-Aldrich Bold Modified Basal Freshwater Nutrient Solution, 50x, after autoclaving.

Composition

flowchart LR
    CultureMech_015878["bold_s_basal_medium_bbm"]
    ing0_Agar["Agar"]
    CultureMech_015878 --> ing0_Agar
    CHEBI_2509["CHEBI:2509"]
    ing0_Agar -.-> CHEBI_2509
    ing1_Distilled_water["Distilled water"]
    CultureMech_015878 --> ing1_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing1_Distilled_water -.-> CHEBI_15377
    ing2_Sigma_Aldrich_Bold_Modified_Basal_Freshwater_Nutrient_Solution__50x["Sigma-Aldrich Bold Modified Basal Freshwater Nutrient Solution, 50x"]
    CultureMech_015878 --> ing2_Sigma_Aldrich_Bold_Modified_Basal_Freshwater_Nutrient_Solution__50x

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 Agar 20 G_PER_L CHEBI:2509
agar
 JCM Medium 1481 lists 20 g agar per liter.
2 Distilled water
H2O 18.015 g/mol
980 ML_PER_L CHEBI:15377
water
 JCM Medium 1481 lists 980 ml distilled water per liter.
3 Sigma-Aldrich Bold Modified Basal Freshwater Nutrient Solution, 50x 20 ML_PER_L JCM Medium 1481 says to add 20 ml of this 50x commercial BBM stock after autoclaving.

Preparation

  1. Mix 20 g agar into 980 ml distilled water.
  2. Autoclave at 121 C for 15 min.
  3. After autoclaving, aseptically add 20 ml Sigma-Aldrich Bold Modified Basal Freshwater Nutrient Solution, 50x.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-06-02T07:09:30.690848+00:00 jcm-grmd-import Scraped from JCM GRMD database Direct import from JCM GRMD=1481. Ingredients captured verbatim; CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps.
2026-06-03T02:00:03.578976Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-06-03T07:12:22.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-09-10T00:00:00-07:00 repair_jcm_1481_bbm_score20.py RESOLVED_JCM_1481_BBM_SCORE20 JCM Medium 1481 lists 20 g agar and 980 ml distilled water for one liter of Bold's Basal Medium (BBM) and instructs adding 20 ml Sigma-Aldrich Bold Modified Basal Freshwater Nutrient Solution, 50x, after autoclaving.