r2a_agar_ph_5_5

CultureMech:015880 bacterial COMPLEX SOLID_AGAR
Original name: R2A AGAR (pH 5.5)

Overview

External ontology
jcm.grmd:1438 (source link unavailable) — R2A AGAR (pH 5.5)
Applications
  • Microbial cultivation
pH
5.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1438 | Derivative medium: ingredients inherited from JCM GRMD=346; apply the stated modification (see preparation_steps) before use.

Composition

flowchart LR
    CultureMech_015880["r2a_agar_ph_5_5"]
    ing0_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_015880 --> ing0_Yeast_extract__BD_Difco_
    ing1_Proteose_peptone_No__3__BD_Difco_["Proteose peptone No. 3 (BD-Difco)"]
    CultureMech_015880 --> ing1_Proteose_peptone_No__3__BD_Difco_
    ing2_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
    CultureMech_015880 --> ing2_Casamino_acids__BD_Difco_
    ing3_Glucose["Glucose"]
    CultureMech_015880 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Soluble_starch["Soluble starch"]
    CultureMech_015880 --> ing4_Soluble_starch
    ing5_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_015880 --> ing5_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing5_Sodium_pyruvate -.-> CHEBI_50144
    ing6_K2HPO4["K2HPO4"]
    CultureMech_015880 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_015880 --> ing7_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing7_MgSO4_7H2O -.-> CHEBI_31795
    ing8_Agar["Agar"]
    CultureMech_015880 --> ing8_Agar
    CHEBI_2509["CHEBI:2509"]
    ing8_Agar -.-> CHEBI_2509
    ing9_Distilled_water["Distilled water"]
    CultureMech_015880 --> ing9_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing9_Distilled_water -.-> CHEBI_15377

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 Yeast extract (BD-Difco) 0.5 G_PER_L
2 Proteose peptone No. 3 (BD-Difco) 0.5 G_PER_L
3 Casamino acids (BD-Difco) 0.5 G_PER_L
4 Glucose
C6H12O6 180.156 g/mol
0.5 G_PER_L CHEBI:17234
glucose
5 Soluble starch 0.5 G_PER_L
6 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
0.3 G_PER_L CHEBI:50144
sodium pyruvate
7 K2HPO4
HO4P.2K 174.174 g/mol
0.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
8 MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
0.05 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
9 Agar 15.0 G_PER_L CHEBI:2509
agar
10 Distilled water
H2O 18.015 g/mol
1.0 ML_PER_L CHEBI:15377
water

Preparation

  1. Prepare R2A agar (see Medium No.
  2. 346 ) and adjust pH to 5.5.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-06-02T07:13:39.857592+00:00 jcm-grmd-import Scraped from JCM GRMD database Direct import from JCM GRMD=1438. Derivative of GRMD=346: ingredients inherited from the base medium, stated modification preserved in preparation_steps and still to be applied. CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps.
2026-06-03T02:00:03.460446Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-06-03T07:12:22.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers