{"cross_mech":[],"family":{"alphafold_models":37191,"candidate_reasons":["pfam_previous_unknown_name"],"characterization_status":"","context_evidence_count":null,"cross_mech":{"example_proteins":0,"protein_traits_record":false,"records_by_mech":{},"scanned":false},"curation_status":"SEEDED","demotion_reasons":[],"description":"This family includes four different proteins from E. coli alone. One of them, YdgG or TqsA, has been shown to mediate transport of the quorum-sensing signal autoinducer 2 (AI-2). It is not clear if TqsA enhances secretion of AI-2 or inhibits AI-2 uptake. By altering the intracellular concentration of AI-2, TqsA affects gene expression in biofilms and biofilm formation [[cite:PUB00076990]]. TsqA belongs to the AI-2 exporter (AI-2E) superfamily [[cite:PUB00076989]].","domain_architectures":79,"interpro_id":"IPR002549","known_evidence_count":null,"matches":58317,"name":"AI-2E family transporter","partial_evidence_count":null,"pfam_id":"PF01594","proteins":58169,"proteomes":19308,"short_name":"AI-2E_transport","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF01594","structures":2,"taxa":29447,"unknown_status":"EX_DUF"},"inputs":{"cross_mech":"cross-mech-duf-examples-2026-10-10","members":"pfam-uniprot-uniref90-2026-10-07","protein_examples":"duf-protein-examples-2026-10-13","worklist":"interpro-pfam-duf-2026-10-10"},"members":[],"metadata":{"curation_status":"SEEDED","local_source":"source/data/families/PF01594.yaml","record":{"characterization_status":"UNSCORED","counters":{"alphafold_models":37191,"domain_architectures":79,"matches":58317,"proteins":58169,"proteomes":19308,"structures":2,"taxa":29447},"curation_status":"SEEDED","description":"This family includes four different proteins from E. coli alone. One of them, YdgG or TqsA, has been shown to mediate transport of the quorum-sensing signal autoinducer 2 (AI-2). It is not clear if TqsA enhances secretion of AI-2 or inhibits AI-2 uptake. By altering the intracellular concentration of AI-2, TqsA affects gene expression in biofilms and biofilm formation [[cite:PUB00076990]]. TsqA belongs to the AI-2 exporter (AI-2E) superfamily [[cite:PUB00076989]].","id":"Pfam:PF01594","interpro_id":"InterPro:IPR002549","name":"AI-2E family transporter","pfam_id":"PF01594","provenance":{"generated_at":"2026-10-10T07:44:21.614711Z","path":"data/worklists/interpro-pfam-duf-2026-10-10.json","sha256":"17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383","snapshot_id":"interpro-pfam-duf-2026-10-10","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/"},"seed_status":"EX_DUF","short_name":"AI-2E_transport","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF01594"},"source":{"commit":"c8155abfcf881ad8662936343deb81e604e3a194","path":"data/families/PF01594.yaml","repository":"https://github.com/CultureBotAI/DUFMech","sha256":"bbe461d5bd181d4497cf596b576d7d10924810ed24dfdff15805cbf1c25ee7f1"}},"protein_examples":{"candidate_count":6,"discovery_scanned":true,"example_count":6,"examples":[{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Bacillus subtilis (strain 168)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/pfam/ai-2e-transport-pf01594.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF01594","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/pfam/ai-2e-transport-pf01594.yaml"}],"pfam_ids":["PF01594"],"protein_name":"Putative transport protein YubA","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"METLQTWSGRFKRFFLDNKFVLFLLVLLLIGLNILVFTKTSFIFTPIIVLLKTISLPIILTGIVFYLLNPVVDFLERRRIRRIYSILLLYLLVIGLITITIVSIIPFLKEQIMSLIDNIPRYVDVVENQTKQLIGSNFVNQAQQTMNINISDLATKVSDQAATIVNSTFTGVGNFIGALTEIIISIVTVPFILFYLLKDGRKLPVYILKFVPTRLKEQTYTVLSEMNHRLSSYIRGQIIVSFCIGFLLFIGYLIIGLDYASLLAVIAACTSIVPYLGPTIAITPAIIIAIVTSPLMLLKLVIVWTIVQLIEGKLISPQIMGKNLHIHPITIIFLLLTAGKLFGVVGIILAIPGYAVAKVITTHLFDWFKMQSHLYDEEKNENTPGHKV","sequence_length":388,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"556279a10847310a4f2d36a890d87adf29ccec126e7df51e7731f99712d3d17a","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/O32086.json","taxon_id":"224308","uniprot_accession":"O32086","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Bacillus subtilis (strain 168)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/protein_family/ncbifam/sporulation-integral-membrane-protein-ytvi-tigr02872.yaml","qualification_status":"NOT_ASSESSED","record_id":"NCBIfam:TIGR02872","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/protein_family/ncbifam/sporulation-integral-membrane-protein-ytvi-tigr02872.yaml"}],"pfam_ids":["PF01594"],"protein_name":"Putative transport protein YtvI","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MNQSYITIFFRTLFVISMTAGSIAAAYYSFPLTYPFLIALILSSVIHPVVDYLDKVTGFPRTINVLGVLAFFLLAAFGVLTILVAEIVTGTAYLAKTLPPHISTFISYCEKLFTTHIQPLYNELTLLFQELETNQQASIVTHIQTLGDSAAKNAGLLLSHILEMIPRFFALLPNTAAVLIFSLLATFFMTKDWHKLKAMLVLILPDRVTANSKAISSELKKAMTGFIKAQAVLVFITMVIVFIGLSLLKVEHAATIAFLIGLVDLLPYLGAGSVFVPWILYLSITGQLPQAIGIGILYLVVLIQRQLTEPKILSKSIGIDPLATLIALFAGFKLFGFLGLIAGPAVLVIIQAFITTGALKEIWSYITVQQK","sequence_length":371,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"ac0b356ff466a432ddf832484b705b4fe272dc8f8da8de616b83a39fd97cd26e","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/O34991.json","taxon_id":"224308","uniprot_accession":"O34991","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Escherichia coli (strain K12)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/pathway/go/autoinducer-ai-2-transmembrane-transport-go1905887.yaml","qualification_status":"NOT_ASSESSED","record_id":"GO:1905887","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/pathway/go/autoinducer-ai-2-transmembrane-transport-go1905887.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/protein_family/ncbifam/ai-2e-family-transporter-nf008930.yaml","qualification_status":"NOT_ASSESSED","record_id":"NCBIfam:NF008930","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/protein_family/ncbifam/ai-2e-family-transporter-nf008930.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/pfam/ai-2e-transport-pf01594.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF01594","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/pfam/ai-2e-transport-pf01594.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["ptm_registry_exact_uniprot_pfam_xref"],"mech":"ProteinTraitsMech","path":"data/grounding/protein_registry.jsonl","qualification_status":"NOT_ASSESSED_FOR_THIS_FAMILY","record_id":"UniProtKB:P0AFS5","section":"protein_registry","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/grounding/protein_registry.jsonl"}],"pfam_ids":["PF01594"],"protein_name":"AI-2 transport protein TqsA","registry_source":{"commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","path":"data/grounding/protein_registry.jsonl","repository":"https://github.com/CultureBotAI/proteintraitsmech","sequence_sha256":"52af8caa6f7aa691c5f446e3e6a5ca1e76e3af420b716808049464b7837b3cbf","sequence_version":1,"sha256":"1be0cf02b76b03a8fe9b80bcead9cd4a419157116eb8da038f266bcc33015eb9","uniprot_release":"2026_03","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/grounding/protein_registry.jsonl"},"registry_status":"EXACT_SEQUENCE_VERSION_MATCH","reviewed":true,"sequence":"MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIAVILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIMTPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLFMLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAALDVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFGNILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSIAVLLSDLNKE","sequence_length":344,"sequence_origin":"PTM_REGISTRY_MIRROR","sequence_sha256":"52af8caa6f7aa691c5f446e3e6a5ca1e76e3af420b716808049464b7837b3cbf","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/P0AFS5.json","taxon_id":"83333","uniprot_accession":"P0AFS5","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Escherichia coli (strain K12)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/protein_family/ncbifam/ai-2e-family-transporter-ydik-nf008216.yaml","qualification_status":"NOT_ASSESSED","record_id":"NCBIfam:NF008216","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/protein_family/ncbifam/ai-2e-family-transporter-ydik-nf008216.yaml"}],"pfam_ids":["PF01594"],"protein_name":"Putative transport protein YdiK","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTVVIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISSGDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHIGRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGVVVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTTWGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLLAVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK","sequence_length":370,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"acccc54e1286806af3ac08d5426c400f750ba619254d713215b51f76419b5233","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/P0AFS7.json","taxon_id":"83333","uniprot_accession":"P0AFS7","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Escherichia coli (strain K12)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/protein_family/ncbifam/ai-2e-family-transporter-nf008930.yaml","qualification_status":"NOT_ASSESSED","record_id":"NCBIfam:NF008930","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/protein_family/ncbifam/ai-2e-family-transporter-nf008930.yaml"}],"pfam_ids":["PF01594"],"protein_name":"Putative transport protein YhhT","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFAIVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELTRKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVFMLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELMGVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIGNILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKLAILLGPGRPKSRLPG","sequence_length":349,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"d27457d9ae215e2a1583d13141238ab26c2d3670f11a6746eb2dfd3046b23a86","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/P0AGM0.json","taxon_id":"83333","uniprot_accession":"P0AGM0","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Homo sapiens","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/pfam/ai-2e-transport-pf01594.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF01594","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/pfam/ai-2e-transport-pf01594.yaml"}],"pfam_ids":["PF01594"],"protein_name":"Transmembrane protein 245","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MADGGGPKDAPSLRSSPGPAPRVPRAVGPSGGGGETPRTAALALRFDKPIKQAFYNTGAVLFVCLCCGAAVLVYFILEAFLRPLLWAVLCGTFLHPFKSSLTRLGRHWLQRLHRAHTPIVLAALLLPLCFVDYGVEALGEQALRRRRLLLLLGAGGPLLYGLYCLGSYLGVQVLLVHAATLICRGLDYFSSLWIWTLVVGYVLTVSFKWNASTERYLRAVSIPVWIILLFHLASLAGSWRIPVFLVIVFLMSVGTLYEKQNGKESSGAELPGQVISMAASTLANLAISITGYESSSEDQPSTQPAEAVDRGESAPTLSTSPSPSSPSPTSPSPTLGRRRPEIGTFLRKKKTSDIYFVSLVWAIVVMQIWLNLWIVQLLPVPIAVWILKKLVIHFGVVDFLEKRYHVWWGIIESFLKERQGALAPWPIVGLGKFLLKVDSKLWHWLNKKMIIWLEKMLDKIISIFIIFLLVIGTLLLALLLTAKVHQESVHMIEVTSNLINETLANHPEWANWLPEAQVVQRALNSAANNVYQYGREWITHKLHKILGDKVNNTAVIEKQVLELWDRLYHSWFVKNVTHSGRHKGQKLHVSRQNSWLGDILDWQDIVSFVHENIETFLSILESLWIVMSRNVSLLFTTVTTLLTILFYSGTALLNFVLSLIIFLTTLFYLLSSSDEYYKPVKWVISLTPLSQPGPSSNIIGQSVEEAIRGVFDASLKMAGFYGLYTWLTHTMFGINIVFIPSALAAILGAVPFLGTYWAAVPAVLDLWLTQGLGCKAILLLIFHLLPTYFVDTAIYSDISGGGHPYLTGLAVAGGAYYLGLEGAIIGPILLCILVVASNIYSAMLVSPTNSVPTPNQTPWPAQPQRTFRDISEDLKSSVG","sequence_length":879,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"f54f1dd2e6a6f59ae95eaaab5056312105aef04f0e46bc2b59d7611b2d0efde4","sequence_version":3,"source_url":"https://rest.uniprot.org/uniprotkb/Q9H330.json","taxon_id":"9606","uniprot_accession":"Q9H330","uniprot_release":"2026_03"}],"pfam_id":"PF01594","short_name":"AI-2E_transport","status":"HAS_SEQUENCE_EXAMPLE","unresolved":[]}}