{"cross_mech":[],"family":{"alphafold_models":2928,"candidate_reasons":["pfam_previous_unknown_name"],"characterization_status":"","context_evidence_count":null,"cross_mech":{"example_proteins":0,"protein_traits_record":false,"records_by_mech":{},"scanned":false},"curation_status":"SEEDED","demotion_reasons":[],"description":"Members of this family may be involved in the activation of nickel-pincer cofactor-dependent enzymes. LarC from Lactobacillus plantarum is involved, together with LarB and LarE, in the synthesis of the enzyme-bound cofactor of lactate racemase (LarA). Larc C binds Ni2+, and functions in nickel delivery to pyridinium-3,5-bisthiocarboxylic acid mononucleotide (P2TMN), to form the mature cofactor [[cite:PUB00085157],[cite:PUB00086644]].","domain_architectures":19,"interpro_id":"IPR002822","known_evidence_count":null,"matches":4175,"name":"Nickel insertion protein","partial_evidence_count":null,"pfam_id":"PF01969","proteins":4090,"proteomes":3294,"short_name":"Ni_insertion","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF01969","structures":5,"taxa":6143,"unknown_status":"EX_DUF"},"inputs":{"cross_mech":"cross-mech-duf-examples-2026-10-10","members":"pfam-uniprot-uniref90-2026-10-07","protein_examples":"duf-protein-examples-2026-10-13","worklist":"interpro-pfam-duf-2026-10-10"},"members":[],"metadata":{"curation_status":"SEEDED","local_source":"source/data/families/PF01969.yaml","record":{"characterization_status":"UNSCORED","counters":{"alphafold_models":2928,"domain_architectures":19,"matches":4175,"proteins":4090,"proteomes":3294,"structures":5,"taxa":6143},"curation_status":"SEEDED","description":"Members of this family may be involved in the activation of nickel-pincer cofactor-dependent enzymes. LarC from Lactobacillus plantarum is involved, together with LarB and LarE, in the synthesis of the enzyme-bound cofactor of lactate racemase (LarA). Larc C binds Ni2+, and functions in nickel delivery to pyridinium-3,5-bisthiocarboxylic acid mononucleotide (P2TMN), to form the mature cofactor [[cite:PUB00085157],[cite:PUB00086644]].","id":"Pfam:PF01969","interpro_id":"InterPro:IPR002822","name":"Nickel insertion protein","pfam_id":"PF01969","provenance":{"generated_at":"2026-10-10T07:44:21.614711Z","path":"data/worklists/interpro-pfam-duf-2026-10-10.json","sha256":"17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383","snapshot_id":"interpro-pfam-duf-2026-10-10","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/"},"seed_status":"EX_DUF","short_name":"Ni_insertion","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF01969"},"source":{"commit":"c8155abfcf881ad8662936343deb81e604e3a194","path":"data/families/PF01969.yaml","repository":"https://github.com/CultureBotAI/DUFMech","sha256":"daf8b18c5e211a7bcc4e4e2353d8cfd08ded0f620c9846dcd0822138ea972ce0"}},"protein_examples":{"candidate_count":1,"discovery_scanned":true,"example_count":1,"examples":[{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/protein_family/ncbifam/nickel-pincer-cofactor-biosynthesis-protein-larc-tigr00299.yaml","qualification_status":"NOT_ASSESSED","record_id":"NCBIfam:TIGR00299","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/protein_family/ncbifam/nickel-pincer-cofactor-biosynthesis-protein-larc-tigr00299.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/family/pfam/ni-insertion-pf01969.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF01969","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/family/pfam/ni-insertion-pf01969.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/structure/homologous_superfamily/cath/mk0293-like-domain-3-10-20-300.yaml","qualification_status":"NOT_ASSESSED","record_id":"CATH:3.10.20.300","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/structure/homologous_superfamily/cath/mk0293-like-domain-3-10-20-300.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/structure/homologous_superfamily/cath/transcriptional-regulatory-protein-pf0864-domain-like-3-30-70-1380.yaml","qualification_status":"NOT_ASSESSED","record_id":"CATH:3.30.70.1380","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/structure/homologous_superfamily/cath/transcriptional-regulatory-protein-pf0864-domain-like-3-30-70-1380.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["ptm_registry_exact_uniprot_pfam_xref"],"mech":"ProteinTraitsMech","path":"data/grounding/protein_registry.jsonl","qualification_status":"NOT_ASSESSED_FOR_THIS_FAMILY","record_id":"UniProtKB:Q58782","section":"protein_registry","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/grounding/protein_registry.jsonl"}],"pfam_ids":["PF01969"],"protein_name":"Putative nickel insertion protein","registry_source":{"commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","path":"data/grounding/protein_registry.jsonl","repository":"https://github.com/CultureBotAI/proteintraitsmech","sequence_sha256":"9a4fe6dabfe464f48f8fc55868378d8d769114cac98b5bc69e8f24f7b394b11f","sequence_version":1,"sha256":"1be0cf02b76b03a8fe9b80bcead9cd4a419157116eb8da038f266bcc33015eb9","uniprot_release":"2026_02","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/grounding/protein_registry.jsonl"},"registry_status":"EXACT_SEQUENCE_VERSION_MATCH","reviewed":true,"sequence":"MFLLDPFSGISGDMFLSAMIDFVDKEDFINTIKKVIDVEIEIKKVKKCHILANKVNIIPKCINCNANTYKDIKNVIKSSDIQEDIKITALEILKILAEAESKVHNVDVENVHFHEVGNYDTIADIVGAAYIINKLNLKNNCLYKPINVGNGFVRTEHGLLPVPAPATAEILKGLKIFFSDINEELTTPTGSAIIKYINPKLAKGAFIIKEVSYGAGDKDLNLLNALRVFRVEDIKREDIVLLETNVDDISAEILGYLYEVLDGKVRDLHFIPTYMKKNRPAYTIRAIVDRDIAEEVAKIIMRETGSLGVRIFDIERITADREFKTIKLFDESVRLKVGRVNDEIISQKPEFEDLKNIAKKYGIPLKDLYKLINISQIKN","sequence_length":379,"sequence_origin":"PTM_REGISTRY_MIRROR","sequence_sha256":"9a4fe6dabfe464f48f8fc55868378d8d769114cac98b5bc69e8f24f7b394b11f","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/Q58782.json","taxon_id":"243232","uniprot_accession":"Q58782","uniprot_release":"2026_03"}],"pfam_id":"PF01969","short_name":"Ni_insertion","status":"HAS_SEQUENCE_EXAMPLE","unresolved":[]}}