{"cross_mech":[],"family":{"alphafold_models":3433,"candidate_reasons":["pfam_previous_unknown_name"],"characterization_status":"","context_evidence_count":null,"cross_mech":{"example_proteins":0,"protein_traits_record":false,"records_by_mech":{},"scanned":false},"curation_status":"SEEDED","demotion_reasons":[],"description":"F420-0:Gamma-glutamyl ligase ([ec:6.3.2.-]) is an enzyme involved in F420 biosynthesis pathway. It catalyses the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0). This reaction produces polyglutamated F420 derivatives. GTP + F420-0 + n L-glutamate -> GDP + phosphate + F420-n","domain_architectures":14,"interpro_id":"IPR002847","known_evidence_count":null,"matches":5452,"name":"F420-0:Gamma-glutamyl ligase","partial_evidence_count":null,"pfam_id":"PF01996","proteins":5328,"proteomes":4806,"short_name":"F420_ligase","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF01996","structures":6,"taxa":6852,"unknown_status":"EX_DUF"},"inputs":{"cross_mech":"cross-mech-duf-examples-2026-10-10","members":"pfam-uniprot-uniref90-2026-10-07","protein_examples":"duf-protein-examples-2026-10-13","worklist":"interpro-pfam-duf-2026-10-10"},"members":[],"metadata":{"curation_status":"SEEDED","local_source":"source/data/families/PF01996.yaml","record":{"characterization_status":"UNSCORED","counters":{"alphafold_models":3433,"domain_architectures":14,"matches":5452,"proteins":5328,"proteomes":4806,"structures":6,"taxa":6852},"curation_status":"SEEDED","description":"F420-0:Gamma-glutamyl ligase ([ec:6.3.2.-]) is an enzyme involved in F420 biosynthesis pathway. It catalyses the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0). This reaction produces polyglutamated F420 derivatives. GTP + F420-0 + n L-glutamate -> GDP + phosphate + F420-n","id":"Pfam:PF01996","interpro_id":"InterPro:IPR002847","name":"F420-0:Gamma-glutamyl ligase","pfam_id":"PF01996","provenance":{"generated_at":"2026-10-10T07:44:21.614711Z","path":"data/worklists/interpro-pfam-duf-2026-10-10.json","sha256":"17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383","snapshot_id":"interpro-pfam-duf-2026-10-10","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/"},"seed_status":"EX_DUF","short_name":"F420_ligase","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF01996"},"source":{"commit":"c8155abfcf881ad8662936343deb81e604e3a194","path":"data/families/PF01996.yaml","repository":"https://github.com/CultureBotAI/DUFMech","sha256":"6a765fd6e424f2d79a1fc641b0e2e4e269ca1ea774c1f1003f1af955e134d06d"}},"protein_examples":{"candidate_count":4,"discovery_scanned":true,"example_count":4,"examples":[{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/family/pfam/f420-ligase-pf01996.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF01996","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/family/pfam/f420-ligase-pf01996.yaml"}],"pfam_ids":["PF00881","PF01996"],"protein_name":"Bifunctional F420 biosynthesis protein FbiB","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MTGPEHGSASTIEILPVIGLPEFRPGDDLSAAVAAAAPWLRDGDVVVVTSKVVSKCEGRLVPAPEDPEQRDRLRRKLIEDEAVRVLARKDRTLITENRLGLVQAAAGVDGSNVGRSELALLPVDPDASAATLRAGLRERLGVTVAVVITDTMGRAWRNGQTDAAVGAAGLAVLRNYAGVRDPYGNELVVTEVAVADEIAAAADLVKGKLTATPVAVVRGFGVSDDGSTARQLLRPGANDLFWLGTAEALELGRQQAQLLRRSVRRFSTDPVPGDLVEAAVAEALTAPAPHHTRPTRFVWLQTPAIRARLLDRMKDKWRSDLTSDGLPADAIERRVARGQILYDAPEVVIPMLVPDGAHSYPDAARTDAEHTMFTVAVGAAVQALLVALAVRGLGSCWIGSTIFAADLVRDELDLPVDWEPLGAIAIGYADEPSGLRDPVPAADLLILK","sequence_length":448,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"b78e7a91f901684422d56e616cdeb2b635bb8cd02a9c6d6416470cbf1697396d","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/P9WP79.json","taxon_id":"83332","uniprot_accession":"P9WP79","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/molecular_function/go/coenzyme-f420-0-l-glutamate-ligase-activity-go0052618.yaml","qualification_status":"NOT_ASSESSED","record_id":"GO:0052618","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/molecular_function/go/coenzyme-f420-0-l-glutamate-ligase-activity-go0052618.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/molecular_function/go/coenzyme-f420-1-gamma-l-glutamate-ligase-activity-go0052619.yaml","qualification_status":"NOT_ASSESSED","record_id":"GO:0052619","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/molecular_function/go/coenzyme-f420-1-gamma-l-glutamate-ligase-activity-go0052619.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/pathway/go/f420-0-metabolic-process-go0052645.yaml","qualification_status":"NOT_ASSESSED","record_id":"GO:0052645","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/pathway/go/f420-0-metabolic-process-go0052645.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/protein_family/ncbifam/coenzyme-f420-0-l-glutamate-ligase-nf009809.yaml","qualification_status":"NOT_ASSESSED","record_id":"NCBIfam:NF009809","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/protein_family/ncbifam/coenzyme-f420-0-l-glutamate-ligase-nf009809.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/protein_family/ncbifam/coenzyme-f420-0-l-glutamate-ligase-tigr01916.yaml","qualification_status":"NOT_ASSESSED","record_id":"NCBIfam:TIGR01916","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/protein_family/ncbifam/coenzyme-f420-0-l-glutamate-ligase-tigr01916.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/interpro/coenzyme-f420-l-glutamate-ligase-like-domain-ipr002847.yaml","qualification_status":"NOT_ASSESSED","record_id":"InterPro:IPR002847","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/interpro/coenzyme-f420-l-glutamate-ligase-like-domain-ipr002847.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/family/pfam/f420-ligase-pf01996.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF01996","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/family/pfam/f420-ligase-pf01996.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/structure/homologous_superfamily/cath/cofe-like-3-30-1330-100.yaml","qualification_status":"NOT_ASSESSED","record_id":"CATH:3.30.1330.100","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/structure/homologous_superfamily/cath/cofe-like-3-30-1330-100.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/structure/homologous_superfamily/cath/cofe-like-domain-3-90-1660-10.yaml","qualification_status":"NOT_ASSESSED","record_id":"CATH:3.90.1660.10","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/structure/homologous_superfamily/cath/cofe-like-domain-3-90-1660-10.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["ptm_registry_exact_uniprot_pfam_xref"],"mech":"ProteinTraitsMech","path":"data/grounding/protein_registry.jsonl","qualification_status":"NOT_ASSESSED_FOR_THIS_FAMILY","record_id":"UniProtKB:Q58178","section":"protein_registry","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/grounding/protein_registry.jsonl"}],"pfam_ids":["PF01996"],"protein_name":"Coenzyme F420:L-glutamate ligase","registry_source":{"commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","path":"data/grounding/protein_registry.jsonl","repository":"https://github.com/CultureBotAI/proteintraitsmech","sequence_sha256":"f6497782154bdae0af08d848191f31689871a978cf6fb73cda0f13b15703a628","sequence_version":1,"sha256":"1be0cf02b76b03a8fe9b80bcead9cd4a419157116eb8da038f266bcc33015eb9","uniprot_release":"2026_03","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/grounding/protein_registry.jsonl"},"registry_status":"EXACT_SEQUENCE_VERSION_MATCH","reviewed":true,"sequence":"MIKEKRKVEVIGLELPIFKGGEQINLSELIAQYPIEDGDIIVIAETLISKLEGGVIDRDKIIPSKEAIELAKKTGKDPKVVQVILDEAKEIVKVGKNFIITETKHGFVCANSGVDESNIYKGIKILPKNPDESAEKIRKEIEKLTGKRVGVIISDSVGRPFRKGAVGIAIGVSGILALWDRKGEKDLFGRELKTTEVAIADELASMANVVMGEADEGIPVVIIRGANVPFGNGKGRDLIRPKEEDVFRN","sequence_length":249,"sequence_origin":"PTM_REGISTRY_MIRROR","sequence_sha256":"f6497782154bdae0af08d848191f31689871a978cf6fb73cda0f13b15703a628","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/Q58178.json","taxon_id":"243232","uniprot_accession":"Q58178","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/molecular_function/go/coenzyme-f420-0-l-glutamate-ligase-activity-go0052618.yaml","qualification_status":"NOT_ASSESSED","record_id":"GO:0052618","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/molecular_function/go/coenzyme-f420-0-l-glutamate-ligase-activity-go0052618.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/interpro/coenzyme-f420-l-glutamate-ligase-like-domain-ipr002847.yaml","qualification_status":"NOT_ASSESSED","record_id":"InterPro:IPR002847","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/interpro/coenzyme-f420-l-glutamate-ligase-like-domain-ipr002847.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/structure/homologous_superfamily/cath/cofe-like-3-30-1330-100.yaml","qualification_status":"NOT_ASSESSED","record_id":"CATH:3.30.1330.100","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/structure/homologous_superfamily/cath/cofe-like-3-30-1330-100.yaml"}],"pfam_ids":["PF01996"],"protein_name":"Uncharacterized protein MJ1361","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MRAYPIKTRYIKRGENFIPIVVEAIKNSGIKLEDGDFVVLSEKMVSTAEGNFIDESKFKPGVLAYLCYYWSKYLWGYVLGKLLKVKEDKIKNLRRMPKEETLKHKQTIIEIVGLRYALKPYAEGGVDLTNVPGTYACPLPKNPKKWAEELYKEIKKELGVDVVVMVADTDATYRVLNFYFTALPYAIDGIISGIGVFGFILGRLADVLKIGGFAGCTPLAIAGNEVYKKYSIGELTRIAFICDRVHKTIKNINEVLEKYNTYVITEEILEKLEHTPVVVVKIKEEYKPESQR","sequence_length":292,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"6453c825b4daec1256d016cd338665a09d2aee83c1eacb771c58398c30ebc58b","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/Q58756.json","taxon_id":"243232","uniprot_accession":"Q58756","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/family/pfam/f420-ligase-pf01996.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF01996","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/family/pfam/f420-ligase-pf01996.yaml"}],"pfam_ids":["PF00881","PF01996"],"protein_name":"Bifunctional F420 biosynthesis protein FbiB","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MTGPEHGSASTIEILPVIGLPEFRPGDDLSAAVAAAAPWLRDGDVVVVTSKVVSKCEGRLVPAPEDPEQRDRLRRKLIEDEAVRVLARKDRTLITENRLGLVQAAAGVDGSNVGRSELALLPVDPDASAATLRAGLRERLGVTVAVVITDTMGRAWRNGQTDAAVGAAGLAVLRNYAGVRDPYGNELVVTEVAVADEIAAAADLVKGKLTATPVAVVRGFGVSDDGSTARQLLRPGANDLFWLGTAEALELGRQQAQLLRRSVRRFSTDPVPGDLVEAAVAEALTAPAPHHTRPTRFVWLQTPAIRARLLDRMKDKWRSDLTSDGLPADAIERRVARGQILYDAPEVVIPMLVPDGAHSYPDAARTDAEHTMFTVAVGAAVQALLVALAVRGLGSCWIGSTIFAADLVRDELDLPVDWEPLGAIAIGYADEPSGLRDPVPAADLLILK","sequence_length":448,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"b78e7a91f901684422d56e616cdeb2b635bb8cd02a9c6d6416470cbf1697396d","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/Q7TWV3.json","taxon_id":"233413","uniprot_accession":"Q7TWV3","uniprot_release":"2026_03"}],"pfam_id":"PF01996","short_name":"F420_ligase","status":"HAS_SEQUENCE_EXAMPLE","unresolved":[]}}