{"cross_mech":[],"family":{"alphafold_models":9472,"candidate_reasons":["pfam_previous_unknown_name"],"characterization_status":"","context_evidence_count":null,"cross_mech":{"example_proteins":0,"protein_traits_record":false,"records_by_mech":{},"scanned":false},"curation_status":"SEEDED","demotion_reasons":[],"description":"This family of proteins are predicted to be SPOUT methyltransferases [[cite:PUB00044735]].","domain_architectures":23,"interpro_id":"IPR003742","known_evidence_count":null,"matches":13956,"name":"Predicted SPOUT methyltransferase","partial_evidence_count":null,"pfam_id":"PF02590","proteins":13939,"proteomes":13252,"short_name":"SPOUT_MTase","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF02590","structures":11,"taxa":19630,"unknown_status":"EX_DUF"},"inputs":{"cross_mech":"cross-mech-duf-examples-2026-10-10","members":"pfam-uniprot-uniref90-2026-10-07","protein_examples":"duf-protein-examples-2026-10-13","worklist":"interpro-pfam-duf-2026-10-10"},"members":[],"metadata":{"curation_status":"SEEDED","local_source":"source/data/families/PF02590.yaml","record":{"characterization_status":"UNSCORED","counters":{"alphafold_models":9472,"domain_architectures":23,"matches":13956,"proteins":13939,"proteomes":13252,"structures":11,"taxa":19630},"curation_status":"SEEDED","description":"This family of proteins are predicted to be SPOUT methyltransferases [[cite:PUB00044735]].","id":"Pfam:PF02590","interpro_id":"InterPro:IPR003742","name":"Predicted SPOUT methyltransferase","pfam_id":"PF02590","provenance":{"generated_at":"2026-10-10T07:44:21.614711Z","path":"data/worklists/interpro-pfam-duf-2026-10-10.json","sha256":"17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383","snapshot_id":"interpro-pfam-duf-2026-10-10","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/"},"seed_status":"EX_DUF","short_name":"SPOUT_MTase","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF02590"},"source":{"commit":"c8155abfcf881ad8662936343deb81e604e3a194","path":"data/families/PF02590.yaml","repository":"https://github.com/CultureBotAI/DUFMech","sha256":"bae02c4efd05fb3aeee32d45c2595d0175cdc83d2621b1b8d199fd6f30e6333f"}},"protein_examples":{"candidate_count":3,"discovery_scanned":true,"example_count":3,"examples":[{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Escherichia coli (strain K12)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/molecular_function/go/rrna-pseudouridine-n3-methyltransferase-activity-go0070038.yaml","qualification_status":"NOT_ASSESSED","record_id":"GO:0070038","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/molecular_function/go/rrna-pseudouridine-n3-methyltransferase-activity-go0070038.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/protein_family/ncbifam/23s-rrna-pseudouridine-1915-n-3-methyltransferase-rlmh-nf000984.yaml","qualification_status":"NOT_ASSESSED","record_id":"NCBIfam:NF000984","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/protein_family/ncbifam/23s-rrna-pseudouridine-1915-n-3-methyltransferase-rlmh-nf000984.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/protein_family/ncbifam/23s-rrna-pseudouridine-1915-n-3-methyltransferase-rlmh-nf000986.yaml","qualification_status":"NOT_ASSESSED","record_id":"NCBIfam:NF000986","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/protein_family/ncbifam/23s-rrna-pseudouridine-1915-n-3-methyltransferase-rlmh-nf000986.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/protein_family/ncbifam/23s-rrna-pseudouridine-1915-n-3-methyltransferase-rlmh-tigr00246.yaml","qualification_status":"NOT_ASSESSED","record_id":"NCBIfam:TIGR00246","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/protein_family/ncbifam/23s-rrna-pseudouridine-1915-n-3-methyltransferase-rlmh-tigr00246.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/cdd/23s-rrna-pseudouridine1915-n3-methyltransferase-rlmh-23s-rrna-pseudour-cd18081.yaml","qualification_status":"NOT_ASSESSED","record_id":"CDD:cd18081","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/cdd/23s-rrna-pseudouridine1915-n3-methyltransferase-rlmh-23s-rrna-pseudour-cd18081.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/family/pfam/spout-mtase-pf02590.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF02590","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/family/pfam/spout-mtase-pf02590.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["ptm_registry_exact_uniprot_pfam_xref"],"mech":"ProteinTraitsMech","path":"data/grounding/protein_registry.jsonl","qualification_status":"NOT_ASSESSED_FOR_THIS_FAMILY","record_id":"UniProtKB:P0A8I8","section":"protein_registry","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/grounding/protein_registry.jsonl"}],"pfam_ids":["PF02590"],"protein_name":"Ribosomal RNA large subunit methyltransferase H","registry_source":{"commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","path":"data/grounding/protein_registry.jsonl","repository":"https://github.com/CultureBotAI/proteintraitsmech","sequence_sha256":"245c4bdd2c6151c9b71bd04f30ff319c0085aa2a5c806a7bbb5ad3909d8d8a19","sequence_version":1,"sha256":"1be0cf02b76b03a8fe9b80bcead9cd4a419157116eb8da038f266bcc33015eb9","uniprot_release":"2026_03","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/grounding/protein_registry.jsonl"},"registry_status":"EXACT_SEQUENCE_VERSION_MATCH","reviewed":true,"sequence":"MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRGKNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLIGGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE","sequence_length":155,"sequence_origin":"PTM_REGISTRY_MIRROR","sequence_sha256":"245c4bdd2c6151c9b71bd04f30ff319c0085aa2a5c806a7bbb5ad3909d8d8a19","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/P0A8I8.json","taxon_id":"83333","uniprot_accession":"P0A8I8","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Bacillus subtilis (strain 168)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/molecular_function/go/rrna-pseudouridine-n3-methyltransferase-activity-go0070038.yaml","qualification_status":"NOT_ASSESSED","record_id":"GO:0070038","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/molecular_function/go/rrna-pseudouridine-n3-methyltransferase-activity-go0070038.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/protein_family/ncbifam/23s-rrna-pseudouridine-1915-n-3-methyltransferase-rlmh-nf000985.yaml","qualification_status":"NOT_ASSESSED","record_id":"NCBIfam:NF000985","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/protein_family/ncbifam/23s-rrna-pseudouridine-1915-n-3-methyltransferase-rlmh-nf000985.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/protein_family/ncbifam/23s-rrna-pseudouridine-1915-n-3-methyltransferase-rlmh-tigr00246.yaml","qualification_status":"NOT_ASSESSED","record_id":"NCBIfam:TIGR00246","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/protein_family/ncbifam/23s-rrna-pseudouridine-1915-n-3-methyltransferase-rlmh-tigr00246.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/cdd/23s-rrna-pseudouridine1915-n3-methyltransferase-rlmh-23s-rrna-pseudour-cd18081.yaml","qualification_status":"NOT_ASSESSED","record_id":"CDD:cd18081","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/cdd/23s-rrna-pseudouridine1915-n3-methyltransferase-rlmh-23s-rrna-pseudour-cd18081.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/family/pfam/spout-mtase-pf02590.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF02590","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/family/pfam/spout-mtase-pf02590.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["ptm_registry_exact_uniprot_pfam_xref"],"mech":"ProteinTraitsMech","path":"data/grounding/protein_registry.jsonl","qualification_status":"NOT_ASSESSED_FOR_THIS_FAMILY","record_id":"UniProtKB:Q45601","section":"protein_registry","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/grounding/protein_registry.jsonl"}],"pfam_ids":["PF02590"],"protein_name":"Ribosomal RNA large subunit methyltransferase H","registry_source":{"commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","path":"data/grounding/protein_registry.jsonl","repository":"https://github.com/CultureBotAI/proteintraitsmech","sequence_sha256":"f25be8718e23b60853cc334167f3d3bd9814e08f7364e0a376cd2d7d6d9bc07f","sequence_version":1,"sha256":"1be0cf02b76b03a8fe9b80bcead9cd4a419157116eb8da038f266bcc33015eb9","uniprot_release":"2026_03","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/grounding/protein_registry.jsonl"},"registry_status":"EXACT_SEQUENCE_VERSION_MATCH","reviewed":true,"sequence":"MNINIVTIGKLKEKYLKQGIEEYTKRLSAYAKIDIIELPDEKAPENLSDQDMKIIKDKEGDRILSKISPDAHVIALAIEGKMKTSEELADTIDKLATYGKSKVTFVIGGSLGLSDTVMKRADEKLSFSKMTFPHQLMRLILVEQIYRAFRINRGEPYHK","sequence_length":159,"sequence_origin":"PTM_REGISTRY_MIRROR","sequence_sha256":"f25be8718e23b60853cc334167f3d3bd9814e08f7364e0a376cd2d7d6d9bc07f","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/Q45601.json","taxon_id":"224308","uniprot_accession":"Q45601","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Arabidopsis thaliana","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/molecular_function/go/rrna-pseudouridine-n3-methyltransferase-activity-go0070038.yaml","qualification_status":"NOT_ASSESSED","record_id":"GO:0070038","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/molecular_function/go/rrna-pseudouridine-n3-methyltransferase-activity-go0070038.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/cdd/23s-rrna-pseudouridine1915-n3-methyltransferase-rlmh-23s-rrna-pseudour-cd18081.yaml","qualification_status":"NOT_ASSESSED","record_id":"CDD:cd18081","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/cdd/23s-rrna-pseudouridine1915-n3-methyltransferase-rlmh-23s-rrna-pseudour-cd18081.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/family/pfam/spout-mtase-pf02590.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF02590","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/family/pfam/spout-mtase-pf02590.yaml"}],"pfam_ids":["PF02590"],"protein_name":"Putative RNA methyltransferase At5g10620","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MSISVMANSHLNQPYASASPTPQEKGRTCRYAGQAVRALPIRVITVGKKRSEGVRLLVDEYKIKLKPYCSFEDSLVRSNPRNAQDVRAQVEDEEVAMMKLIGSDDWVVVLDERGRDIDSEQMAELLGDAGNSGASRISFCIGGAYGHGTQVRKRANVTIRLSSMVLNHQIALVVLMEQLYRSWTILKGQNYHH","sequence_length":193,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"0096cfb0cdc41e713218e7fa8ccb717479597db0f241762f1c2a7ddbbe543a7a","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/Q9LXB4.json","taxon_id":"3702","uniprot_accession":"Q9LXB4","uniprot_release":"2026_03"}],"pfam_id":"PF02590","short_name":"SPOUT_MTase","status":"HAS_SEQUENCE_EXAMPLE","unresolved":[]}}