{"cross_mech":[],"family":{"alphafold_models":2010,"candidate_reasons":["pfam_previous_unknown_name"],"characterization_status":"","context_evidence_count":null,"cross_mech":{"example_proteins":0,"protein_traits_record":false,"records_by_mech":{},"scanned":false},"curation_status":"SEEDED","demotion_reasons":[],"description":"In methanobacteria (2R)-phospho-3-sulfolactate synthase (ComA) catalyses the first step of the biosynthesis of coenzyme M from phosphoenolpyruvate (P-enolpyruvate). This novel enzyme catalyses the stereospecific Michael addition of sulfite to P-enolpyruvate, forming L-2-phospho-3-sulfolactate (PSL). It is suggested that the ComA-catalysed reaction is analogous to those reactions catalysed by beta-elimination enzymes that proceed through an enolate intermediate [[cite:PUB00010730]].","domain_architectures":37,"interpro_id":"IPR003830","known_evidence_count":null,"matches":2796,"name":"(2R)-phospho-3-sulfolactate synthase (ComA)","partial_evidence_count":null,"pfam_id":"PF02679","proteins":2768,"proteomes":2472,"short_name":"ComA","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF02679","structures":3,"taxa":4674,"unknown_status":"EX_DUF"},"inputs":{"cross_mech":"cross-mech-duf-examples-2026-10-10","members":"pfam-uniprot-uniref90-2026-10-07","protein_examples":"duf-protein-examples-2026-10-13","worklist":"interpro-pfam-duf-2026-10-10"},"members":[],"metadata":{"curation_status":"SEEDED","local_source":"source/data/families/PF02679.yaml","record":{"characterization_status":"UNSCORED","counters":{"alphafold_models":2010,"domain_architectures":37,"matches":2796,"proteins":2768,"proteomes":2472,"structures":3,"taxa":4674},"curation_status":"SEEDED","description":"In methanobacteria (2R)-phospho-3-sulfolactate synthase (ComA) catalyses the first step of the biosynthesis of coenzyme M from phosphoenolpyruvate (P-enolpyruvate). This novel enzyme catalyses the stereospecific Michael addition of sulfite to P-enolpyruvate, forming L-2-phospho-3-sulfolactate (PSL). It is suggested that the ComA-catalysed reaction is analogous to those reactions catalysed by beta-elimination enzymes that proceed through an enolate intermediate [[cite:PUB00010730]].","id":"Pfam:PF02679","interpro_id":"InterPro:IPR003830","name":"(2R)-phospho-3-sulfolactate synthase (ComA)","pfam_id":"PF02679","provenance":{"generated_at":"2026-10-10T07:44:21.614711Z","path":"data/worklists/interpro-pfam-duf-2026-10-10.json","sha256":"17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383","snapshot_id":"interpro-pfam-duf-2026-10-10","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/"},"seed_status":"EX_DUF","short_name":"ComA","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF02679"},"source":{"commit":"c8155abfcf881ad8662936343deb81e604e3a194","path":"data/families/PF02679.yaml","repository":"https://github.com/CultureBotAI/DUFMech","sha256":"b4a819416d103ad386cf0d076b3cce32432c3f6c9c39b3ba592665568cbd1d5d"}},"protein_examples":{"candidate_count":3,"discovery_scanned":true,"example_count":3,"examples":[{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Bacillus subtilis (strain 168)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/molecular_function/go/phosphosulfolactate-synthase-activity-go0043817.yaml","qualification_status":"NOT_ASSESSED","record_id":"GO:0043817","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/molecular_function/go/phosphosulfolactate-synthase-activity-go0043817.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/family/pfam/coma-pf02679.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF02679","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/family/pfam/coma-pf02679.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/homologous_superfamily/interpro/2r-phospho-3-sulpholactate-synthase-coma-superfamily-ipr036112.yaml","qualification_status":"NOT_ASSESSED","record_id":"InterPro:IPR036112","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/homologous_superfamily/interpro/2r-phospho-3-sulpholactate-synthase-coma-superfamily-ipr036112.yaml"}],"pfam_ids":["PF02679"],"protein_name":"Phosphosulfolactate synthase","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MNDFSLELPVRTNKPRETGQSILIDNGYPLQFFKDAIAGASDYIDFVKFGWGTSLLTKDLEEKISTLKEHDITFFFGGTLFEKYVSQKKVNEFHRYCTYFGCEYIEISNGTLPMTNKEKAAYIADFSDEFLVLSEVGSKDAELASRQSSEEWLEYIVEDMEAGAEKVITEARESGTGGICSSSGDVRFQIVDDIISSDIDINRLIFEAPNKTLQQGFIQKIGPNVNLANIPFHDAIALETLRLGLRSDTFFL","sequence_length":252,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"4eea7f5dd12f912eb003b979e112de8076a85b1e90d2930b2f0c879d268ec425","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/O06739.json","taxon_id":"224308","uniprot_accession":"O06739","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/molecular_function/go/phosphosulfolactate-synthase-activity-go0043817.yaml","qualification_status":"NOT_ASSESSED","record_id":"GO:0043817","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/molecular_function/go/phosphosulfolactate-synthase-activity-go0043817.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/molecular_function/go/sulfopyruvate-decarboxylase-activity-go0050545.yaml","qualification_status":"NOT_ASSESSED","record_id":"GO:0050545","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/molecular_function/go/sulfopyruvate-decarboxylase-activity-go0050545.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/pathway/go/coenzyme-m-biosynthetic-process-go0019295.yaml","qualification_status":"NOT_ASSESSED","record_id":"GO:0019295","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/pathway/go/coenzyme-m-biosynthetic-process-go0019295.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/protein_family/ncbifam/phosphosulfolactate-synthase-tigr03849.yaml","qualification_status":"NOT_ASSESSED","record_id":"NCBIfam:TIGR03849","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/protein_family/ncbifam/phosphosulfolactate-synthase-tigr03849.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/family/pfam/coma-pf02679.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF02679","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/family/pfam/coma-pf02679.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/homologous_superfamily/interpro/2r-phospho-3-sulpholactate-synthase-coma-superfamily-ipr036112.yaml","qualification_status":"NOT_ASSESSED","record_id":"InterPro:IPR036112","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/homologous_superfamily/interpro/2r-phospho-3-sulpholactate-synthase-coma-superfamily-ipr036112.yaml"}],"pfam_ids":["PF02679"],"protein_name":"Phosphosulfolactate synthase","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MKAFEFLYEDFQRGLTVVLDKGLPPKFVEDYLKVCGDYIDFVKFGWGTSAVIDRDVVKEKINYYKDWGIKVYPGGTLFEYAYSKGKFDEFLNECEKLGFEAVEISDGSSDISLEERKNAIKRAKDNGFMVLTEVGKKMPDKDKQLTIDDRIKLINFDLDAGADYVIIEGRESGKGIGLFDKEGKVKENELDVLAKNVDINKVIFEAPQKSQQVAFILKFGSSVNLANIAFDEVISLETLRRGLRGDTFGKV","sequence_length":251,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"1a5bddf4429d40bd6c3683aa3902432d68fb98e8338f92db947d8435d12945b5","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/Q57703.json","taxon_id":"243232","uniprot_accession":"Q57703","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Arabidopsis thaliana","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/family/pfam/coma-pf02679.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF02679","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/family/pfam/coma-pf02679.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/homologous_superfamily/interpro/2r-phospho-3-sulpholactate-synthase-coma-superfamily-ipr036112.yaml","qualification_status":"NOT_ASSESSED","record_id":"InterPro:IPR036112","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/homologous_superfamily/interpro/2r-phospho-3-sulpholactate-synthase-coma-superfamily-ipr036112.yaml"}],"pfam_ids":["PF02679"],"protein_name":"Protein HEAT-STRESS-ASSOCIATED 32","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MAAYYRWKSFEENEDRPEKPRRYGVTEMRGPHYSVLSQNLLQEIFESMGQFVDGLKFSGGSNSLIPKSFIKQAIEMAHEHGVYVSTGDWAEHMLRSGPSAFKDYVEECKQLGFDTIELNANLLEVPEETLLRYVRLIKNGGLRAKPMFAVKFNKSDIPGRNRAFGSYVVPEPRSSEFVEDIDLLIRKAERCLEAGADTIMIDADDVCKYADSLRADIIAKVIGRLGIEKTMFEASDAKLVEWFIKRYGPNVNLYVDHSQIMDLECLRGRHLGKDHQSVLSSSYFLF","sequence_length":286,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"2c339d4e704e3f2f20e51d7cd471ef7ce853681e954aa5264f55e8dda6f4e62a","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/Q8GWL1.json","taxon_id":"3702","uniprot_accession":"Q8GWL1","uniprot_release":"2026_03"}],"pfam_id":"PF02679","short_name":"ComA","status":"HAS_SEQUENCE_EXAMPLE","unresolved":[]}}