{"cross_mech":[],"family":{"alphafold_models":164,"candidate_reasons":["pfam_previous_unknown_name"],"characterization_status":"","context_evidence_count":null,"cross_mech":{"example_proteins":0,"protein_traits_record":false,"records_by_mech":{},"scanned":false},"curation_status":"SEEDED","demotion_reasons":[],"description":"LIN-8 is a nuclear protein, present at the sites of transcriptional repressor complexes, which interacts with LIN-35 Rb. Lin35 Rb is a product of the class B synMuv gene lin-35 which silences genes required for vulval specification through chromatin modification and remodelling [[cite:PUB00083139]]. The biological role of the interaction has not yet been determined however predictions have been made. The interaction shows that class A synMuv genes control vulval induction through the transcriptional regulation of gene expression. LIN-8 normally functions as part of a protein complex however when the complex is absent, other family members can partially replace LIN-8 activity [[cite:PUB00083139]].","domain_architectures":17,"interpro_id":"IPR005020","known_evidence_count":null,"matches":203,"name":"Ras-mediated vulval-induction antagonist","partial_evidence_count":null,"pfam_id":"PF03353","proteins":166,"proteomes":10,"short_name":"Lin-8","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF03353","structures":0,"taxa":26,"unknown_status":"EX_DUF"},"inputs":{"cross_mech":"cross-mech-duf-examples-2026-10-10","members":"pfam-uniprot-uniref90-2026-10-07","protein_examples":"duf-protein-examples-2026-10-13","worklist":"interpro-pfam-duf-2026-10-10"},"members":[],"metadata":{"curation_status":"SEEDED","local_source":"source/data/families/PF03353.yaml","record":{"characterization_status":"UNSCORED","counters":{"alphafold_models":164,"domain_architectures":17,"matches":203,"proteins":166,"proteomes":10,"structures":0,"taxa":26},"curation_status":"SEEDED","description":"LIN-8 is a nuclear protein, present at the sites of transcriptional repressor complexes, which interacts with LIN-35 Rb. Lin35 Rb is a product of the class B synMuv gene lin-35 which silences genes required for vulval specification through chromatin modification and remodelling [[cite:PUB00083139]]. The biological role of the interaction has not yet been determined however predictions have been made. The interaction shows that class A synMuv genes control vulval induction through the transcriptional regulation of gene expression. LIN-8 normally functions as part of a protein complex however when the complex is absent, other family members can partially replace LIN-8 activity [[cite:PUB00083139]].","id":"Pfam:PF03353","interpro_id":"InterPro:IPR005020","name":"Ras-mediated vulval-induction antagonist","pfam_id":"PF03353","provenance":{"generated_at":"2026-10-10T07:44:21.614711Z","path":"data/worklists/interpro-pfam-duf-2026-10-10.json","sha256":"17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383","snapshot_id":"interpro-pfam-duf-2026-10-10","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/"},"seed_status":"EX_DUF","short_name":"Lin-8","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF03353"},"source":{"commit":"c8155abfcf881ad8662936343deb81e604e3a194","path":"data/families/PF03353.yaml","repository":"https://github.com/CultureBotAI/DUFMech","sha256":"2f197b7bdf58310f44288300e731c882fc522923ba6646f613933dd6f0ded9f4"}},"protein_examples":{"candidate_count":2,"discovery_scanned":true,"example_count":2,"examples":[{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Caenorhabditis elegans","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/family/pfam/lin-8-pf03353.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF03353","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/family/pfam/lin-8-pf03353.yaml"}],"pfam_ids":["PF03353"],"protein_name":"Protein lin-8","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MSKIKTHSTGSKRTVPFYKLPPPVPLPPLPPPDPTRYFSTEKYIALSKDEKFKFDDYDVNDETLKKVVLNEIGKCPDIWSSRSQAAIMEHYPIVATETYRRTGLLLSIKSLKQIYKCGKDNLRNRLRVAIVSKRLTPAQVEAYMWRWEFYGFIRYYRDYTQRWEADLLKDLDVVLGLEARRASKNMEKVDSGELMEPMEPMDSTMDEMCVEEEPYEETGSNWSDPAPEPSQSKSQSPEAKYPQAYLLPEADEVYNPDDFYQEEHESASNAMYRIAFSQQYGGGGSPAVQKPVTFSAQPAPAPVREAPSPVVENVSSSSFTPKPPAMINNFGEEMNQITYQAIRIAREQPERLKLLRKALFDVVLAFDQKEYADVGDLYRDLAQKNS","sequence_length":386,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"c02ad6f561af977afbc7c9707c11823fc3c23fe5149ef98aa3070240d149a625","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/G5EDW7.json","taxon_id":"6239","uniprot_accession":"G5EDW7","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Caenorhabditis elegans","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/family/pfam/lin-8-pf03353.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF03353","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/family/pfam/lin-8-pf03353.yaml"}],"pfam_ids":["PF03353"],"protein_name":"Uncharacterized lin-8 family protein lido-16","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MDSPFNFTRRRSHVVANERRDMIQPSSSIPRPITNNIWTLNDYLNFDKSDVHTGPPRSLEIKKLILDTLKEMPELWTQRCSKKPHWELLGFNILQRTGMRIGTCQLMETFRNARKHVNAKIRKCWKEGLSKAETEIKCSNWDLFENFRFFFEFKCRHWQFPDDCDDLVVELDTEEGIISNPSLPPNEVCDQNVEILQSATKESIMPDVPEHYLDFEQHLKAKIQQALNKHPGQEKLLKNAIFTTLNGIENRDHKSLEELFSSLSSNH","sequence_length":267,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"10f2e24ab58bc04ae72322d8542ae359ac4ecb3449305a5dee571eb455495590","sequence_version":2,"source_url":"https://rest.uniprot.org/uniprotkb/Q10026.json","taxon_id":"6239","uniprot_accession":"Q10026","uniprot_release":"2026_03"}],"pfam_id":"PF03353","short_name":"Lin-8","status":"HAS_SEQUENCE_EXAMPLE","unresolved":[]}}