{"cross_mech":[{"cited_uniprot_accession":"","family_mentioned_in_record":true,"link_basis":["canonical_example_family_classification"],"pfam_id":"PF03994","protein_label":"UPF0719 inner membrane protein YjfL","reviewed":true,"short_name":"DUF350","source_category":"sequence/repeat/interpro","source_mech":"ProteinTraitsMech","source_path":"data/traits/sequence/repeat/interpro/protein-of-unknown-function-duf350-ipr007140.yaml","source_record_id":"InterPro:IPR007140","source_record_label":"Protein of unknown function DUF350","source_section":"canonical_examples","taxon_id":"NCBITaxon:83333","taxon_label":"Escherichia coli (strain K12)","uniprot_accession":"P0AF80","unknown_status":"UNKNOWN_CANDIDATE"},{"cited_uniprot_accession":"","family_mentioned_in_record":true,"link_basis":["canonical_example_family_classification"],"pfam_id":"PF03994","protein_label":"UPF0719 transmembrane protein YshE","reviewed":true,"short_name":"DUF350","source_category":"sequence/repeat/interpro","source_mech":"ProteinTraitsMech","source_path":"data/traits/sequence/repeat/interpro/protein-of-unknown-function-duf350-ipr007140.yaml","source_record_id":"InterPro:IPR007140","source_record_label":"Protein of unknown function DUF350","source_section":"canonical_examples","taxon_id":"NCBITaxon:224308","taxon_label":"Bacillus subtilis (strain 168)","uniprot_accession":"P94546","unknown_status":"UNKNOWN_CANDIDATE"},{"cited_uniprot_accession":"","family_mentioned_in_record":null,"link_basis":["trait_identifier"],"pfam_id":"PF03994","protein_label":"","reviewed":null,"short_name":"DUF350","source_category":"sequence/repeat/interpro","source_mech":"ProteinTraitsMech","source_path":"data/traits/sequence/repeat/interpro/protein-of-unknown-function-duf350-ipr007140.yaml","source_record_id":"InterPro:IPR007140","source_record_label":"Protein of unknown function DUF350","source_section":"trait_identifier","taxon_id":"","taxon_label":"","uniprot_accession":"","unknown_status":"UNKNOWN_CANDIDATE"},{"cited_uniprot_accession":"","family_mentioned_in_record":true,"link_basis":["canonical_example_family_classification"],"pfam_id":"PF03994","protein_label":"UPF0719 inner membrane protein YjfL","reviewed":true,"short_name":"DUF350","source_category":"sequence/repeat/pfam","source_mech":"ProteinTraitsMech","source_path":"data/traits/sequence/repeat/pfam/duf350-pf03994.yaml","source_record_id":"Pfam:PF03994","source_record_label":"Domain of Unknown Function (DUF350)","source_section":"canonical_examples","taxon_id":"NCBITaxon:83333","taxon_label":"Escherichia coli (strain K12)","uniprot_accession":"P0AF80","unknown_status":"UNKNOWN_CANDIDATE"},{"cited_uniprot_accession":"","family_mentioned_in_record":true,"link_basis":["canonical_example_family_classification"],"pfam_id":"PF03994","protein_label":"UPF0719 inner membrane protein YjfL","reviewed":true,"short_name":"DUF350","source_category":"sequence/repeat/pfam","source_mech":"ProteinTraitsMech","source_path":"data/traits/sequence/repeat/pfam/duf350-pf03994.yaml","source_record_id":"Pfam:PF03994","source_record_label":"Domain of Unknown Function (DUF350)","source_section":"canonical_examples","taxon_id":"NCBITaxon:83334","taxon_label":"Escherichia coli O157:H7","uniprot_accession":"P0AF81","unknown_status":"UNKNOWN_CANDIDATE"},{"cited_uniprot_accession":"","family_mentioned_in_record":true,"link_basis":["canonical_example_family_classification"],"pfam_id":"PF03994","protein_label":"UPF0719 transmembrane protein YshE","reviewed":true,"short_name":"DUF350","source_category":"sequence/repeat/pfam","source_mech":"ProteinTraitsMech","source_path":"data/traits/sequence/repeat/pfam/duf350-pf03994.yaml","source_record_id":"Pfam:PF03994","source_record_label":"Domain of Unknown Function (DUF350)","source_section":"canonical_examples","taxon_id":"NCBITaxon:224308","taxon_label":"Bacillus subtilis (strain 168)","uniprot_accession":"P94546","unknown_status":"UNKNOWN_CANDIDATE"},{"cited_uniprot_accession":"","family_mentioned_in_record":null,"link_basis":["trait_identifier"],"pfam_id":"PF03994","protein_label":"","reviewed":null,"short_name":"DUF350","source_category":"sequence/repeat/pfam","source_mech":"ProteinTraitsMech","source_path":"data/traits/sequence/repeat/pfam/duf350-pf03994.yaml","source_record_id":"Pfam:PF03994","source_record_label":"Domain of Unknown Function (DUF350)","source_section":"trait_identifier","taxon_id":"","taxon_label":"","uniprot_accession":"","unknown_status":"UNKNOWN_CANDIDATE"}],"family":{"alphafold_models":4316,"candidate_reasons":["short_name_matches_duf","name_says_unknown_function","domain_of_unknown_function"],"characterization_status":"","context_evidence_count":null,"cross_mech":{"example_proteins":3,"protein_traits_record":true,"records_by_mech":{"ProteinTraitsMech":2},"scanned":true},"curation_status":"SEEDED","demotion_reasons":[],"description":"This entry represents a family of bacterial proteins that form an integral membrane protein complex. These complexes form an alpha/alpha(8) structure. These proteins are composed of repeats of a two TM helix motif. The function of these proteins is currently unknown.","domain_architectures":7,"interpro_id":"IPR007140","known_evidence_count":null,"matches":7639,"name":"Domain of Unknown Function (DUF350)","partial_evidence_count":null,"pfam_id":"PF03994","proteins":7119,"proteomes":5751,"short_name":"DUF350","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF03994","structures":0,"taxa":7882,"unknown_status":"UNKNOWN_CANDIDATE"},"inputs":{"cross_mech":"cross-mech-duf-examples-2026-10-10","members":"pfam-uniprot-uniref90-2026-10-07","protein_examples":"duf-protein-examples-2026-10-13","worklist":"interpro-pfam-duf-2026-10-10"},"members":[],"metadata":{"curation_status":"SEEDED","local_source":"source/data/families/PF03994.yaml","record":{"characterization_status":"UNSCORED","counters":{"alphafold_models":4316,"domain_architectures":7,"matches":7639,"proteins":7119,"proteomes":5751,"structures":0,"taxa":7882},"curation_status":"SEEDED","description":"This entry represents a family of bacterial proteins that form an integral membrane protein complex. These complexes form an alpha/alpha(8) structure. These proteins are composed of repeats of a two TM helix motif. The function of these proteins is currently unknown.","id":"Pfam:PF03994","interpro_id":"InterPro:IPR007140","name":"Domain of Unknown Function (DUF350)","pfam_id":"PF03994","provenance":{"generated_at":"2026-10-10T07:44:21.614711Z","path":"data/worklists/interpro-pfam-duf-2026-10-10.json","sha256":"17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383","snapshot_id":"interpro-pfam-duf-2026-10-10","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/"},"seed_status":"UNKNOWN_CANDIDATE","short_name":"DUF350","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF03994"},"source":{"commit":"c8155abfcf881ad8662936343deb81e604e3a194","path":"data/families/PF03994.yaml","repository":"https://github.com/CultureBotAI/DUFMech","sha256":"f6a1d38d7cf05c3c36db318ec4837572cbda4725b1df869469eb6ced93935065"}},"protein_examples":{"candidate_count":3,"discovery_scanned":true,"example_count":3,"examples":[{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Escherichia coli (strain K12)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/repeat/interpro/protein-of-unknown-function-duf350-ipr007140.yaml","qualification_status":"NOT_ASSESSED","record_id":"InterPro:IPR007140","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/repeat/interpro/protein-of-unknown-function-duf350-ipr007140.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/repeat/pfam/duf350-pf03994.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF03994","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/repeat/pfam/duf350-pf03994.yaml"}],"pfam_ids":["PF03994"],"protein_name":"UPF0719 inner membrane protein YjfL","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNNTAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW","sequence_length":132,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"f20824cabaa4a6f11ff248314d65fac582f21d19312d733c1ad92f09e7d966a9","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/P0AF80.json","taxon_id":"83333","uniprot_accession":"P0AF80","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Escherichia coli O157:H7","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/repeat/pfam/duf350-pf03994.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF03994","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/repeat/pfam/duf350-pf03994.yaml"}],"pfam_ids":["PF03994"],"protein_name":"UPF0719 inner membrane protein YjfL","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNNTAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW","sequence_length":132,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"f20824cabaa4a6f11ff248314d65fac582f21d19312d733c1ad92f09e7d966a9","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/P0AF81.json","taxon_id":"83334","uniprot_accession":"P0AF81","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Bacillus subtilis (strain 168)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/repeat/interpro/protein-of-unknown-function-duf350-ipr007140.yaml","qualification_status":"NOT_ASSESSED","record_id":"InterPro:IPR007140","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/repeat/interpro/protein-of-unknown-function-duf350-ipr007140.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/repeat/pfam/duf350-pf03994.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF03994","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/repeat/pfam/duf350-pf03994.yaml"}],"pfam_ids":["PF03994"],"protein_name":"UPF0719 transmembrane protein YshE","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MSDFWENELVEIAAYYSVAVLCLVLFLTVFELVTAYKNWEEIQKGNLAVAMATGGKILGIANVFQHSISQHNSLLQMIGWGVYGFVMLLISYFIFEFLTPRFKIDQEIENDNRAVGFISFVISVGLSFVVAAGI","sequence_length":134,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"b749fb2bf1d5edeade22570140d022e86fe2e4214a35daa60e502f81c58cbd80","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/P94546.json","taxon_id":"224308","uniprot_accession":"P94546","uniprot_release":"2026_03"}],"pfam_id":"PF03994","short_name":"DUF350","status":"HAS_SEQUENCE_EXAMPLE","unresolved":[]}}