{"cross_mech":[],"family":{"alphafold_models":2341,"candidate_reasons":["pfam_previous_unknown_name"],"characterization_status":"","context_evidence_count":null,"cross_mech":{"example_proteins":0,"protein_traits_record":false,"records_by_mech":{},"scanned":false},"curation_status":"SEEDED","demotion_reasons":[],"description":"This is a family of bacterial putative uroporphyrinogen-III C-methyltransferase proteins. It forms one of the members of a complex of proteins involved in the biogenesis of the inner membrane in E.coli. Uroporphorphyrin-III C-methyltransferase (HemX) is a single spanning inner membrane protein that regulates the activity of NAD(P)H:glutamyl-tRNA reductase (HemA) in the tetrapyrrole biosynthesis pathway [[cite:PUB00009969],[cite:PUB00054976]].","domain_architectures":15,"interpro_id":"IPR007470","known_evidence_count":null,"matches":3875,"name":"HemX, putative uroporphyrinogen-III C-methyltransferase","partial_evidence_count":null,"pfam_id":"PF04375","proteins":3821,"proteomes":3792,"short_name":"HemX","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF04375","structures":0,"taxa":5052,"unknown_status":"EX_DUF"},"inputs":{"cross_mech":"cross-mech-duf-examples-2026-10-10","members":"pfam-uniprot-uniref90-2026-10-07","protein_examples":"duf-protein-examples-2026-10-13","worklist":"interpro-pfam-duf-2026-10-10"},"members":[],"metadata":{"curation_status":"SEEDED","local_source":"source/data/families/PF04375.yaml","record":{"characterization_status":"UNSCORED","counters":{"alphafold_models":2341,"domain_architectures":15,"matches":3875,"proteins":3821,"proteomes":3792,"structures":0,"taxa":5052},"curation_status":"SEEDED","description":"This is a family of bacterial putative uroporphyrinogen-III C-methyltransferase proteins. It forms one of the members of a complex of proteins involved in the biogenesis of the inner membrane in E.coli. Uroporphorphyrin-III C-methyltransferase (HemX) is a single spanning inner membrane protein that regulates the activity of NAD(P)H:glutamyl-tRNA reductase (HemA) in the tetrapyrrole biosynthesis pathway [[cite:PUB00009969],[cite:PUB00054976]].","id":"Pfam:PF04375","interpro_id":"InterPro:IPR007470","name":"HemX, putative uroporphyrinogen-III C-methyltransferase","pfam_id":"PF04375","provenance":{"generated_at":"2026-10-10T07:44:21.614711Z","path":"data/worklists/interpro-pfam-duf-2026-10-10.json","sha256":"17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383","snapshot_id":"interpro-pfam-duf-2026-10-10","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/"},"seed_status":"EX_DUF","short_name":"HemX","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF04375"},"source":{"commit":"c8155abfcf881ad8662936343deb81e604e3a194","path":"data/families/PF04375.yaml","repository":"https://github.com/CultureBotAI/DUFMech","sha256":"f005d58b40e8feb5dad7c37f4d153b87d1ae6afff313c99ba6869f334ef6c88c"}},"protein_examples":{"candidate_count":3,"discovery_scanned":true,"example_count":3,"examples":[{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/family/pfam/hemx-pf04375.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF04375","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/family/pfam/hemx-pf04375.yaml"}],"pfam_ids":["PF04375"],"protein_name":"Uncharacterized protein HI_0602.1","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MXVNFDETSKNNDKLSNNITDWQQNIEKSATSFLNHFIRISPKQNSNKKELLAPNQDIYLRENIRLRLQLAIMAVPRQQNELYKQSLEAVSSWVRSYFDTNAEVTQNFLKLVDGLTDTSIYVDVPEQLKSLTLLDKYLNRTALDVQKVEIEADKAIDTMPQVEAVKPTQSESQQ","sequence_length":174,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"bb9e288c7cfdcf93967ab0a6922e77b53be6f070d97cf230d8755ec80ffec50d","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/O86227.json","taxon_id":"71421","uniprot_accession":"O86227","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Escherichia coli (strain K12)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/protein_family/ncbifam/uroporphyrinogen-iii-c-methyltransferase-nf008173.yaml","qualification_status":"NOT_ASSESSED","record_id":"NCBIfam:NF008173","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/protein_family/ncbifam/uroporphyrinogen-iii-c-methyltransferase-nf008173.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/family/pfam/hemx-pf04375.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF04375","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/family/pfam/hemx-pf04375.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["ptm_registry_exact_uniprot_pfam_xref"],"mech":"ProteinTraitsMech","path":"data/grounding/protein_registry.jsonl","qualification_status":"NOT_ASSESSED_FOR_THIS_FAMILY","record_id":"UniProtKB:P09127","section":"protein_registry","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/grounding/protein_registry.jsonl"}],"pfam_ids":["PF04375"],"protein_name":"Protein HemX","registry_source":{"commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","path":"data/grounding/protein_registry.jsonl","repository":"https://github.com/CultureBotAI/proteintraitsmech","sequence_sha256":"4844ff09ffd3630ea7ac72edf557c767764732b778934bbf92a4f24736c4e741","sequence_version":1,"sha256":"1be0cf02b76b03a8fe9b80bcead9cd4a419157116eb8da038f266bcc33015eb9","uniprot_release":"2026_03","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/grounding/protein_registry.jsonl"},"registry_status":"EXACT_SEQUENCE_VERSION_MATCH","reviewed":true,"sequence":"MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAIAIALAAGIGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLKQANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAALLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRLADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQDIYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQLSQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPQADTPAAAPQGE","sequence_length":393,"sequence_origin":"PTM_REGISTRY_MIRROR","sequence_sha256":"4844ff09ffd3630ea7ac72edf557c767764732b778934bbf92a4f24736c4e741","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/P09127.json","taxon_id":"83333","uniprot_accession":"P09127","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/family/pfam/hemx-pf04375.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF04375","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/family/pfam/hemx-pf04375.yaml"}],"pfam_ids":["PF04375"],"protein_name":"Uncharacterized protein HI_0603","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MAKEQPNDLTEQLTDTPKTAVEQAETMQSVPQTIVKKTGTALSLLAILVALGIGGAGYYFGQQQMAKIQQKLTALENQTGANLSSNNTNNNKRLTQLEQSLKTAQENIAQLEQLIVSKTGEITSLQTQMKQVSQLAIAQQPSDWLFSEADFLLNNALRKLVLDNDVDTAVSLLKLADETLVKVNNSQANEIRSAINQDLKQLLSLSSVDQNAIMQKLSQLANTVDELQRL","sequence_length":230,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"a94bab79529d05626001d3c4680c85b1ed37519350ef243eaa020aef22ecf7e2","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/P44773.json","taxon_id":"71421","uniprot_accession":"P44773","uniprot_release":"2026_03"}],"pfam_id":"PF04375","short_name":"HemX","status":"HAS_SEQUENCE_EXAMPLE","unresolved":[]}}