{"cross_mech":[],"family":{"alphafold_models":2101,"candidate_reasons":["pfam_previous_unknown_name"],"characterization_status":"","context_evidence_count":null,"cross_mech":{"example_proteins":0,"protein_traits_record":false,"records_by_mech":{},"scanned":false},"curation_status":"SEEDED","demotion_reasons":[],"description":"This family consists of several short bacterial proteins formely known as (DUF797). It was recently shown that Mycobacterium tuberculosis contains a small protein, Pup (Rv2111c), that is covalently conjugated to the e-NH2 groups of lysines on several target proteins (pupylation) such as the malonyl CoA acyl carrier protein (FabD) [[cite:PUB00053383]]. Pupylation of FabD was shown to result in its recruitment to the mycobacterial proteasome and subsequent degradation analogous to eukaryotic ubiquitin-conjugated proteins. Searches recovered Pup orthologs in all major actinobacteria lineages including the basal bifidobacteria and also sporadically in certain other bacterial lineages. [[cite:PUB00053382]] The Pup proteins were all between 50-90 residues in length and a multiple alignment shows that they all contain a conserved motif with a G [EQ] signature at the C-terminus. Thus, all of them are suitable for conjugation via the terminal glutamate or the deamidated glutamine (as shown in the case of the Mycobacterium Pup [[cite:PUB00053382]]). The conserved globular core of Pup is predicted to form a bihelical unit with the extreme C-terminal 6-7 residues forming a tail in the extended conformation. Thus, Pup is structurally unrelated to the ubiquitin fold and has convergently evolved the function of protein modifier.","domain_architectures":4,"interpro_id":"IPR008515","known_evidence_count":null,"matches":3495,"name":"Pup-like protein","partial_evidence_count":null,"pfam_id":"PF05639","proteins":3495,"proteomes":3401,"short_name":"Pup","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF05639","structures":12,"taxa":4136,"unknown_status":"EX_DUF"},"inputs":{"cross_mech":"cross-mech-duf-examples-2026-10-10","members":"pfam-uniprot-uniref90-2026-10-07","protein_examples":"duf-protein-examples-2026-10-13","worklist":"interpro-pfam-duf-2026-10-10"},"members":[],"metadata":{"curation_status":"SEEDED","local_source":"source/data/families/PF05639.yaml","record":{"characterization_status":"UNSCORED","counters":{"alphafold_models":2101,"domain_architectures":4,"matches":3495,"proteins":3495,"proteomes":3401,"structures":12,"taxa":4136},"curation_status":"SEEDED","description":"This family consists of several short bacterial proteins formely known as (DUF797). It was recently shown that Mycobacterium tuberculosis contains a small protein, Pup (Rv2111c), that is covalently conjugated to the e-NH2 groups of lysines on several target proteins (pupylation) such as the malonyl CoA acyl carrier protein (FabD) [[cite:PUB00053383]]. Pupylation of FabD was shown to result in its recruitment to the mycobacterial proteasome and subsequent degradation analogous to eukaryotic ubiquitin-conjugated proteins. Searches recovered Pup orthologs in all major actinobacteria lineages including the basal bifidobacteria and also sporadically in certain other bacterial lineages. [[cite:PUB00053382]] The Pup proteins were all between 50-90 residues in length and a multiple alignment shows that they all contain a conserved motif with a G [EQ] signature at the C-terminus. Thus, all of them are suitable for conjugation via the terminal glutamate or the deamidated glutamine (as shown in the case of the Mycobacterium Pup [[cite:PUB00053382]]). The conserved globular core of Pup is predicted to form a bihelical unit with the extreme C-terminal 6-7 residues forming a tail in the extended conformation. Thus, Pup is structurally unrelated to the ubiquitin fold and has convergently evolved the function of protein modifier.","id":"Pfam:PF05639","interpro_id":"InterPro:IPR008515","name":"Pup-like protein","pfam_id":"PF05639","provenance":{"generated_at":"2026-10-10T07:44:21.614711Z","path":"data/worklists/interpro-pfam-duf-2026-10-10.json","sha256":"17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383","snapshot_id":"interpro-pfam-duf-2026-10-10","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/"},"seed_status":"EX_DUF","short_name":"Pup","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF05639"},"source":{"commit":"c8155abfcf881ad8662936343deb81e604e3a194","path":"data/families/PF05639.yaml","repository":"https://github.com/CultureBotAI/DUFMech","sha256":"4fda72b20a7121bd9dd5c10229ca55e0d3aafce9dd34046c6e579ae573e905aa"}},"protein_examples":{"candidate_count":0,"discovery_scanned":true,"example_count":0,"examples":[],"pfam_id":"PF05639","short_name":"Pup","status":"NO_SIBLING_CANDIDATE","unresolved":[]}}