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The crystal structure of AlkZ revealed a C-shaped configuration with three tandem winged helix-turn-helix (wHTH) motifs and a positively charged surface that shows residues of great importance for the enzymatic activity [[cite:PUB00100682]].","domain_architectures":19,"interpro_id":"IPR009351","known_evidence_count":null,"matches":17024,"name":"DNA glycosylase AlkZ-like","partial_evidence_count":null,"pfam_id":"PF06224","proteins":16955,"proteomes":7771,"short_name":"AlkZ-like","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF06224","structures":2,"taxa":10387,"unknown_status":"EX_DUF"},"inputs":{"cross_mech":"cross-mech-duf-examples-2026-10-10","members":"pfam-uniprot-uniref90-2026-10-07","protein_examples":"duf-protein-examples-2026-10-13","worklist":"interpro-pfam-duf-2026-10-10"},"members":[],"metadata":{"curation_status":"SEEDED","local_source":"source/data/families/PF06224.yaml","record":{"characterization_status":"UNSCORED","counters":{"alphafold_models":9642,"domain_architectures":19,"matches":17024,"proteins":16955,"proteomes":7771,"structures":2,"taxa":10387},"curation_status":"SEEDED","description":"This protein family includes DNA glycosylase AlkZ from Streptomyces sahachiroi (also known as Azi36) and similar proteins predominantly found in bacteria. AlkZ is an enzyme that provides self-resistance from azinomycin B (AZB) toxicity by unhooking interstrand cross- links (ICLs). The crystal structure of AlkZ revealed a C-shaped configuration with three tandem winged helix-turn-helix (wHTH) motifs and a positively charged surface that shows residues of great importance for the enzymatic activity [[cite:PUB00100682]].","id":"Pfam:PF06224","interpro_id":"InterPro:IPR009351","name":"DNA glycosylase AlkZ-like","pfam_id":"PF06224","provenance":{"generated_at":"2026-10-10T07:44:21.614711Z","path":"data/worklists/interpro-pfam-duf-2026-10-10.json","sha256":"17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383","snapshot_id":"interpro-pfam-duf-2026-10-10","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/"},"seed_status":"EX_DUF","short_name":"AlkZ-like","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF06224"},"source":{"commit":"c8155abfcf881ad8662936343deb81e604e3a194","path":"data/families/PF06224.yaml","repository":"https://github.com/CultureBotAI/DUFMech","sha256":"602707dffd9d3afd16fb315fede8a6b1295e50f140d76645acaca05362c37ef0"}},"protein_examples":{"candidate_count":2,"discovery_scanned":true,"example_count":2,"examples":[{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Streptomyces sahachiroi","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/family/pfam/alkz-like-pf06224.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF06224","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/family/pfam/alkz-like-pf06224.yaml"}],"pfam_ids":["PF06224"],"protein_name":"Interstrand DNA cross-link repair glycosylase","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MKASWRQVFAWRMQRQFLEPRTQPSASDVVGRLCGVQAQVWSVAELNVALRQAAPDRESVNREVADLSLMKTWAMRGTLHLLRPSEAGPYLSLMANTGSWLKPSWTRASGVTPRQVDELTEEVAGILDGVVLTRDELVTRLVADKRFVSMEERLRSGWGSVLKPLAWRGVLCHGPNRGNKITFTLPASQFGADWGKMPEPDEAAPTVIKAYLGAYGPATIETFDRWLSLNSTSKPKLRKWFGDMGDELTEVDVEGRKAFVLTEHAEELAATAPCTGIRLLGGFDQYLLGPGTKDEVVLAPEHRSAVSRAAGWISPVVVKDGRVVGVWEIVDQELVVTPFPDTERLPVKAVEKEAAHVARASGVSRLPVRIV","sequence_length":371,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"32e8b0c3fb288eb670bfc6ea8c4579a692c401160aedaca06d54bb2fc5b9d2c8","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/B4XYC8.json","taxon_id":"285525","uniprot_accession":"B4XYC8","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Escherichia coli (strain K12)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/family/pfam/alkz-like-pf06224.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF06224","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/family/pfam/alkz-like-pf06224.yaml"}],"pfam_ids":["PF06224"],"protein_name":"Interstrand DNA cross-link repair glycosylase","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLLQIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRHRMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPHKRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARSLGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHDDLLPLLERALAGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQLVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGCCPQGLFTDCRTGWEIDPVA","sequence_length":410,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"325059c787b62a189ccb1145826d82414d807d91b4d26a4899073d3d60bac8ef","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/P75843.json","taxon_id":"83333","uniprot_accession":"P75843","uniprot_release":"2026_03"}],"pfam_id":"PF06224","short_name":"AlkZ-like","status":"HAS_SEQUENCE_EXAMPLE","unresolved":[]}}