{"cross_mech":[],"family":{"alphafold_models":7977,"candidate_reasons":["pfam_previous_unknown_name"],"characterization_status":"","context_evidence_count":null,"cross_mech":{"example_proteins":0,"protein_traits_record":false,"records_by_mech":{},"scanned":false},"curation_status":"SEEDED","demotion_reasons":[],"description":"This entry (previously known as DUF1524) represents a domain found C-terminal in the GmrSD family of modification-dependent restriction endonucleases including SspE proteins [[cite:PUB00155638]]. SspE is a dual-function protein that exerts both N-terminal NTPase and C-terminal DNA nicking nuclease activities to defend against phage infection [[cite:PUB00155272]]. SspE has a phosphorothioate (PT) modification-binding preference and utilises epigenetic PT modification to accomplish self-nonself discrimination and to coordinate its NTPase and DNA nicking activities to fight against phages. The domain represented in this entry has a DNA nicking nuclease activity and belongs to HNH nuclease superfamily.","domain_architectures":95,"interpro_id":"IPR011089","known_evidence_count":null,"matches":13171,"name":"GmrSD restriction endonucleases, C-terminal domain","partial_evidence_count":null,"pfam_id":"PF07510","proteins":13168,"proteomes":7699,"short_name":"GmrSD_C","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF07510","structures":7,"taxa":11960,"unknown_status":"EX_DUF"},"inputs":{"cross_mech":"cross-mech-duf-examples-2026-10-10","members":"pfam-uniprot-uniref90-2026-10-07","protein_examples":"duf-protein-examples-2026-10-13","worklist":"interpro-pfam-duf-2026-10-10"},"members":[],"metadata":{"curation_status":"SEEDED","local_source":"source/data/families/PF07510.yaml","record":{"characterization_status":"UNSCORED","counters":{"alphafold_models":7977,"domain_architectures":95,"matches":13171,"proteins":13168,"proteomes":7699,"structures":7,"taxa":11960},"curation_status":"SEEDED","description":"This entry (previously known as DUF1524) represents a domain found C-terminal in the GmrSD family of modification-dependent restriction endonucleases including SspE proteins [[cite:PUB00155638]]. SspE is a dual-function protein that exerts both N-terminal NTPase and C-terminal DNA nicking nuclease activities to defend against phage infection [[cite:PUB00155272]]. SspE has a phosphorothioate (PT) modification-binding preference and utilises epigenetic PT modification to accomplish self-nonself discrimination and to coordinate its NTPase and DNA nicking activities to fight against phages. The domain represented in this entry has a DNA nicking nuclease activity and belongs to HNH nuclease superfamily.","id":"Pfam:PF07510","interpro_id":"InterPro:IPR011089","name":"GmrSD restriction endonucleases, C-terminal domain","pfam_id":"PF07510","provenance":{"generated_at":"2026-10-10T07:44:21.614711Z","path":"data/worklists/interpro-pfam-duf-2026-10-10.json","sha256":"17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383","snapshot_id":"interpro-pfam-duf-2026-10-10","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/"},"seed_status":"EX_DUF","short_name":"GmrSD_C","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF07510"},"source":{"commit":"c8155abfcf881ad8662936343deb81e604e3a194","path":"data/families/PF07510.yaml","repository":"https://github.com/CultureBotAI/DUFMech","sha256":"7767b6bd902038c1bc3cdf0f8fe47eb141582aec66312e39c03701030a0a9b3b"}},"protein_examples":{"candidate_count":2,"discovery_scanned":true,"example_count":2,"examples":[{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/pfam/gmrsd-c-pf07510.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF07510","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/pfam/gmrsd-c-pf07510.yaml"}],"pfam_ids":["PF07510"],"protein_name":"Uncharacterized secreted protein ARB_06907","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MKYTFLAVLSAVTVLATPAPVPTPPNIPSASTAQSLLSGLTVRPQGPQDGYSRDKFPHWITISGTCNTRETVLRRDGTNVQVDGSCAATSGSWFSPYDGATWTAASDVDIDHVVPLSNAWKSGAASWTTSQRQSFANDLSNPQLIAVTDNVNQAKGDQGPESWKPPLQSYWCTYSRMWIKVKSVYDLSVTSAEKSALTSMLNTC","sequence_length":204,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"780fd63b4626db59de2696b9cb646c9aa2b90c573796c3a19ed7b9afa6521517","sequence_version":2,"source_url":"https://rest.uniprot.org/uniprotkb/D4AK18.json","taxon_id":"663331","uniprot_accession":"D4AK18","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Escherichia coli (strain K12)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/interpro/gmrsd-restriction-endonucleases-c-terminal-domain-ipr011089.yaml","qualification_status":"NOT_ASSESSED","record_id":"InterPro:IPR011089","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/interpro/gmrsd-restriction-endonucleases-c-terminal-domain-ipr011089.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/pfam/gmrsd-c-pf07510.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF07510","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/pfam/gmrsd-c-pf07510.yaml"}],"pfam_ids":["PF07510"],"protein_name":"Uncharacterized protein YfjM","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MGDDVHRFGNLFLISPSSNSILSNYSPADKKKFYVETERAESPKQAIMMSYKEWGPDGQGINNIESHEHAMLTLLKEHRDMTLPTRK","sequence_length":87,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"bab841956cf5410a75386435383643f1f18f6d7a79a31365c946f79ee9a21032","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/P52128.json","taxon_id":"83333","uniprot_accession":"P52128","uniprot_release":"2026_03"}],"pfam_id":"PF07510","short_name":"GmrSD_C","status":"HAS_SEQUENCE_EXAMPLE","unresolved":[]}}