{"cross_mech":[],"family":{"alphafold_models":557,"candidate_reasons":["pfam_previous_unknown_name"],"characterization_status":"","context_evidence_count":null,"cross_mech":{"example_proteins":0,"protein_traits_record":false,"records_by_mech":{},"scanned":false},"curation_status":"SEEDED","demotion_reasons":[],"description":"This is the FLD domain found in tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS. TiaS is an archaeal ATP-dependent agmatine transferase that catalyses the formation of 2-agmatinylcytidine (agm2C) at the wobble position (C34) of tRNA(Ile2) [[cite:PUB00056803],[cite:PUB00154283],[cite:PUB00106019]]. This modified base specifically recognises AUA codons. TiaS consists of four domains, the TCKD domain ([pfam:PF22641]) at the N-terminal followed by the FLD domain, an OB fold domain and a zinc ribbon domain (ZRD) domain at the C-terminal [[cite:PUB00154283]]. This domain is responsible for agmantine binding [[cite:PUB00056803]].","domain_architectures":11,"interpro_id":"IPR013696","known_evidence_count":null,"matches":593,"name":"TiaS FLD domain","partial_evidence_count":null,"pfam_id":"PF08489","proteins":592,"proteomes":586,"short_name":"TiaS_FLD","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF08489","structures":7,"taxa":1064,"unknown_status":"EX_DUF"},"inputs":{"cross_mech":"cross-mech-duf-examples-2026-10-10","members":"pfam-uniprot-uniref90-2026-10-07","protein_examples":"duf-protein-examples-2026-10-13","worklist":"interpro-pfam-duf-2026-10-10"},"members":[],"metadata":{"curation_status":"SEEDED","local_source":"source/data/families/PF08489.yaml","record":{"characterization_status":"UNSCORED","counters":{"alphafold_models":557,"domain_architectures":11,"matches":593,"proteins":592,"proteomes":586,"structures":7,"taxa":1064},"curation_status":"SEEDED","description":"This is the FLD domain found in tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS. TiaS is an archaeal ATP-dependent agmatine transferase that catalyses the formation of 2-agmatinylcytidine (agm2C) at the wobble position (C34) of tRNA(Ile2) [[cite:PUB00056803],[cite:PUB00154283],[cite:PUB00106019]]. This modified base specifically recognises AUA codons. TiaS consists of four domains, the TCKD domain ([pfam:PF22641]) at the N-terminal followed by the FLD domain, an OB fold domain and a zinc ribbon domain (ZRD) domain at the C-terminal [[cite:PUB00154283]]. This domain is responsible for agmantine binding [[cite:PUB00056803]].","id":"Pfam:PF08489","interpro_id":"InterPro:IPR013696","name":"TiaS FLD domain","pfam_id":"PF08489","provenance":{"generated_at":"2026-10-10T07:44:21.614711Z","path":"data/worklists/interpro-pfam-duf-2026-10-10.json","sha256":"17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383","snapshot_id":"interpro-pfam-duf-2026-10-10","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/"},"seed_status":"EX_DUF","short_name":"TiaS_FLD","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF08489"},"source":{"commit":"c8155abfcf881ad8662936343deb81e604e3a194","path":"data/families/PF08489.yaml","repository":"https://github.com/CultureBotAI/DUFMech","sha256":"4e61a523fee138cf63258c9cfa4470a249d400e9298b3008156dd88f50548af3"}},"protein_examples":{"candidate_count":1,"discovery_scanned":true,"example_count":1,"examples":[{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/molecular_function/go/ligase-activity-forming-carbon-nitrogen-bonds-go0016879.yaml","qualification_status":"NOT_ASSESSED","record_id":"GO:0016879","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/molecular_function/go/ligase-activity-forming-carbon-nitrogen-bonds-go0016879.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/pathway/go/trna-wobble-cytosine-modification-go0002101.yaml","qualification_status":"NOT_ASSESSED","record_id":"GO:0002101","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/pathway/go/trna-wobble-cytosine-modification-go0002101.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/cdd/rpa2-obf-like-a-subgroup-of-uncharacterized-archaeal-ob-folds-with-sim-cd04482.yaml","qualification_status":"NOT_ASSESSED","record_id":"CDD:cd04482","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/cdd/rpa2-obf-like-a-subgroup-of-uncharacterized-archaeal-ob-folds-with-sim-cd04482.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/interpro/tias-c-terminal-zinc-ribbon-domain-ipr055394.yaml","qualification_status":"NOT_ASSESSED","record_id":"InterPro:IPR055394","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/interpro/tias-c-terminal-zinc-ribbon-domain-ipr055394.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/interpro/tias-fld-domain-ipr013696.yaml","qualification_status":"NOT_ASSESSED","record_id":"InterPro:IPR013696","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/interpro/tias-fld-domain-ipr013696.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/interpro/tias-like-tckd-domain-ipr053870.yaml","qualification_status":"NOT_ASSESSED","record_id":"InterPro:IPR053870","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/interpro/tias-like-tckd-domain-ipr053870.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/pfam/tias-fld-pf08489.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF08489","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/pfam/tias-fld-pf08489.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/pfam/tias-tckd-pf22641.yaml","qualification_status":"NOT_ASSESSED","record_id":"Pfam:PF22641","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/pfam/tias-tckd-pf22641.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/pfam/zn-ribbon-tias-pf23783.yaml","qualification_status":"NOT_ASSESSED","record_id":"Pfam:PF23783","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/pfam/zn-ribbon-tias-pf23783.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/structure/homologous_superfamily/cath/2-40-50-1010-2-40-50-1010.yaml","qualification_status":"NOT_ASSESSED","record_id":"CATH:2.40.50.1010","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/structure/homologous_superfamily/cath/2-40-50-1010-2-40-50-1010.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/structure/homologous_superfamily/cath/3-30-70-2200-3-30-70-2200.yaml","qualification_status":"NOT_ASSESSED","record_id":"CATH:3.30.70.2200","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/structure/homologous_superfamily/cath/3-30-70-2200-3-30-70-2200.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/structure/homologous_superfamily/cath/3-90-600-20-3-90-600-20.yaml","qualification_status":"NOT_ASSESSED","record_id":"CATH:3.90.600.20","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/structure/homologous_superfamily/cath/3-90-600-20-3-90-600-20.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["ptm_registry_exact_uniprot_pfam_xref"],"mech":"ProteinTraitsMech","path":"data/grounding/protein_registry.jsonl","qualification_status":"NOT_ASSESSED_FOR_THIS_FAMILY","record_id":"UniProtKB:Q58495","section":"protein_registry","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/grounding/protein_registry.jsonl"}],"pfam_ids":["PF01336","PF08489","PF22641","PF23783"],"protein_name":"tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS","registry_source":{"commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","path":"data/grounding/protein_registry.jsonl","repository":"https://github.com/CultureBotAI/proteintraitsmech","sequence_sha256":"734b9f3464716f24b03d6892d64d45a7a3da40733bbe73665ea38701c7d66a6c","sequence_version":1,"sha256":"1be0cf02b76b03a8fe9b80bcead9cd4a419157116eb8da038f266bcc33015eb9","uniprot_release":"2026_03","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/grounding/protein_registry.jsonl"},"registry_status":"EXACT_SEQUENCE_VERSION_MATCH","reviewed":true,"sequence":"MVMMFIGIDDTDSPNKYCTTYIATLLIEELKGCGYSVDMPKLIRMNPMVKYKTRGNGGVAIHILDELYSKDKEEIKNITISLVEKYTDFECENTNPGIVFLDEAKYKENREKLTNYYKKVLYDIVSVDYAEKFILKVGGEFIKYKLGRGIIGALGAISSTPPYTYELLAYRKKEMWGKKREIDEKSVIEMDKETFPYTFDNYDYENEKILIAPNTPCPVLFGIRGIDAEILLKAMHKIEGEKPERFMIFKTNHGTDVHLRKMNIKDIYPNTGVIVYGRVVEEPRDIEGGHVIFKLSDGTGEIDCMAYEPTKGFRDIIRKLIVGDYIAVYGTVREKPLGINIEKIKILKLEKKFVKDKRCPYCGGTLKAKGKKAGYKCKKCKKTIAYDEIKMIEVERDLKTGFYEVPGSARRHLSKPIQLIDLI","sequence_length":423,"sequence_origin":"PTM_REGISTRY_MIRROR","sequence_sha256":"734b9f3464716f24b03d6892d64d45a7a3da40733bbe73665ea38701c7d66a6c","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/Q58495.json","taxon_id":"243232","uniprot_accession":"Q58495","uniprot_release":"2026_03"}],"pfam_id":"PF08489","short_name":"TiaS_FLD","status":"HAS_SEQUENCE_EXAMPLE","unresolved":[]}}