{"cross_mech":[],"family":{"alphafold_models":2197,"candidate_reasons":["pfam_previous_unknown_name"],"characterization_status":"","context_evidence_count":null,"cross_mech":{"example_proteins":0,"protein_traits_record":false,"records_by_mech":{},"scanned":false},"curation_status":"SEEDED","demotion_reasons":[],"description":"This domain is found in uncharacterised bacterial proteins, including the putative peptidoglycan hydrolase Rv2525c from Mycobacterium tuberculosis [[cite:PUB00100887],[cite:PUB00100886]]. It has been proposed to function as a peptidoglycan hydrolase with glycosidase activity regarding structural studies showed that this domain has a typical TIM-barrel-like fold, characteristic of glycoside hydrolases [[cite:PUB00100887]]. However, it displays esterase activity toward p-nitrophenyl esters of various acyl chain length in vitro [[cite:PUB00100886]].","domain_architectures":98,"interpro_id":"IPR015020","known_evidence_count":null,"matches":3876,"name":"Rv2525c-like, glycoside hydrolase-like domain","partial_evidence_count":null,"pfam_id":"PF08924","proteins":3876,"proteomes":2610,"short_name":"Rv2525c_GlyHyd-like","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF08924","structures":5,"taxa":3609,"unknown_status":"EX_DUF"},"inputs":{"cross_mech":"cross-mech-duf-examples-2026-10-10","members":"pfam-uniprot-uniref90-2026-10-07","protein_examples":"duf-protein-examples-2026-10-13","worklist":"interpro-pfam-duf-2026-10-10"},"members":[],"metadata":{"curation_status":"SEEDED","local_source":"source/data/families/PF08924.yaml","record":{"characterization_status":"UNSCORED","counters":{"alphafold_models":2197,"domain_architectures":98,"matches":3876,"proteins":3876,"proteomes":2610,"structures":5,"taxa":3609},"curation_status":"SEEDED","description":"This domain is found in uncharacterised bacterial proteins, including the putative peptidoglycan hydrolase Rv2525c from Mycobacterium tuberculosis [[cite:PUB00100887],[cite:PUB00100886]]. It has been proposed to function as a peptidoglycan hydrolase with glycosidase activity regarding structural studies showed that this domain has a typical TIM-barrel-like fold, characteristic of glycoside hydrolases [[cite:PUB00100887]]. However, it displays esterase activity toward p-nitrophenyl esters of various acyl chain length in vitro [[cite:PUB00100886]].","id":"Pfam:PF08924","interpro_id":"InterPro:IPR015020","name":"Rv2525c-like, glycoside hydrolase-like domain","pfam_id":"PF08924","provenance":{"generated_at":"2026-10-10T07:44:21.614711Z","path":"data/worklists/interpro-pfam-duf-2026-10-10.json","sha256":"17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383","snapshot_id":"interpro-pfam-duf-2026-10-10","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/"},"seed_status":"EX_DUF","short_name":"Rv2525c_GlyHyd-like","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF08924"},"source":{"commit":"c8155abfcf881ad8662936343deb81e604e3a194","path":"data/families/PF08924.yaml","repository":"https://github.com/CultureBotAI/DUFMech","sha256":"4e3ba5d93673367532fdbc7be1346b42521ffd8baf96a9f00bdeccaab5fb586d"}},"protein_examples":{"candidate_count":2,"discovery_scanned":true,"example_count":2,"examples":[{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Bacillus subtilis (strain 168)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/cdd/baca-is-a-bacterial-lysin-from-enterococcus-faecalis-that-degrades-bac-cd06418.yaml","qualification_status":"NOT_ASSESSED","record_id":"CDD:cd06418","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/cdd/baca-is-a-bacterial-lysin-from-enterococcus-faecalis-that-degrades-bac-cd06418.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/interpro/rv2525c-like-glycoside-hydrolase-like-domain-ipr015020.yaml","qualification_status":"NOT_ASSESSED","record_id":"InterPro:IPR015020","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/interpro/rv2525c-like-glycoside-hydrolase-like-domain-ipr015020.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/interpro/ybfg-like-c-terminal-domain-ipr048479.yaml","qualification_status":"NOT_ASSESSED","record_id":"InterPro:IPR048479","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/interpro/ybfg-like-c-terminal-domain-ipr048479.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/pfam/rv2525c-glyhyd-like-pf08924.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF08924","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/pfam/rv2525c-glyhyd-like-pf08924.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/pfam/ybfg-c-pf21015.yaml","qualification_status":"NOT_ASSESSED","record_id":"Pfam:PF21015","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/pfam/ybfg-c-pf21015.yaml"}],"pfam_ids":["PF01471","PF08924","PF21015","PF30262"],"protein_name":"Uncharacterized protein YbfG","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MVDEMVLITQQWLNDTYSGKHGYNPVEESGKTGWDTIYGLTRALQIELGISEPADNFGPTTQRLFKPLKRQAPDSKPSNMNFILQGALWCKGFNPGGFTGVFYEKTENAVKEFQKAAGLTTQDGIVTTLIMKALLDMSAFKLVSGGDSRIRQIQQNLNRDYNDYIGLMPCDGLYGRDTNKALIYALQKEEGMSTSVANGFFGNGTTSLCPTLTPGDSRTGFVLIVQYALYCNGKSFDPGEFDGKYGVGVVSAVKAFQEFMCLPQTGYADMPTIKALLSSSGDTTRTASACDTATIITAEKAQTLRNNGYKTVGRYLTGNVRTSSGLTSKALTSKELAVILDAGLKVFPIYQDGGYESSYFVKDQGTRDAYSAASAARRLGFPSGTTIYFAVDFDAYDYEVTDKIIPYFQEIKSAFTKMQTFSTAPKYEIGVYGPRNICIRTSEAGLTKYSFVANMSTGFSGNLGYPMPNNWAFDQFYEGTIGSGSGSIGIDKDGYSGRDSGASNVNPPSDPVYDARLRTLTDILSTIPALENLTSLANAMFEFDTTETIFTSPELDIILSTSLLATIPSEGSPNTITITNGKPGAYITGLLGDTQTSLTASQIDSYQNLLNSLSLSVRNGYLEVYVNPTAESLNIQIKIYTPDIPVGDNVTTGLTTTITFKIKTYKGVPVTSPESELALDWPSYDQYLFPVVGVAALLLIGNMGSDLTNNKGVKVATALSAMLLAIFAYYTS","sequence_length":732,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"31b7bb88c390bd2e87bcfc929c7d9794883c1257fcfb2bbbf2d7b4a1fecc0a8d","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/O31447.json","taxon_id":"224308","uniprot_accession":"O31447","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Bacillus subtilis (strain 168)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/cdd/baca-is-a-bacterial-lysin-from-enterococcus-faecalis-that-degrades-bac-cd06418.yaml","qualification_status":"NOT_ASSESSED","record_id":"CDD:cd06418","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/cdd/baca-is-a-bacterial-lysin-from-enterococcus-faecalis-that-degrades-bac-cd06418.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/interpro/rv2525c-like-glycoside-hydrolase-like-domain-ipr015020.yaml","qualification_status":"NOT_ASSESSED","record_id":"InterPro:IPR015020","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/interpro/rv2525c-like-glycoside-hydrolase-like-domain-ipr015020.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/pfam/rv2525c-glyhyd-like-pf08924.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF08924","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/pfam/rv2525c-glyhyd-like-pf08924.yaml"}],"pfam_ids":["PF01471","PF08924","PF30262"],"protein_name":"Uncharacterized protein FadG","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MDEMVLLTQEWLNETYKGKSGYNSIEENGKTGWKTMYALTRALQLELGITQTSDSFGPTTLRKLKELGPISTSTNSKKNIVKIIQGALYCKGYGPGGLTGTFGQGTKEAIAEMQLHMGLSKTDGVVTPKVFKALLNMDSYILLNGASEKVRSIQQWLNNKYYNRENFYFMPCDGLYSRDTQKSLVYAIQYEEGLSDSIANGNFGPTTQRLIPVLRIGETDEKNSFIHLFQAALIFNGYNVPFDGVYSESVRSKVKAFQSFAKLQQSGTADFQTWASLLVSTGDPNRKGVACDSITQITSDRAESLKRAGYKIVGRYLTNAPGSTLNKKIQPGELETILKSGLNVFPIYQTYGGATNYFNKEQGKKDAFAAYKAAKEYGFKNNTVIYFAVDYDAYGNDLNNNIIPHFEGINEIMNGFLGSTYKIGIYAPRNVCTIVSKKGLAFASFVSGMSTGFSGNLGYPLPYNWAFDQISTITVGNGSGMIEIDNDICSGLDNGVNTINIVPSENKKFFDQIDVLYETAEKYAQMQSDLNNGVKKTQLANELVAQYLRKDDYKGWKWVPTAGQIDPIYREWAVKR","sequence_length":576,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"450246f6cfb4ca6358093f39fb6e98d4964503d88ce5d6660537b527629e97c5","sequence_version":2,"source_url":"https://rest.uniprot.org/uniprotkb/O34320.json","taxon_id":"224308","uniprot_accession":"O34320","uniprot_release":"2026_03"}],"pfam_id":"PF08924","short_name":"Rv2525c_GlyHyd-like","status":"HAS_SEQUENCE_EXAMPLE","unresolved":[]}}