{"cross_mech":[{"cited_uniprot_accession":"","family_mentioned_in_record":null,"link_basis":["record_mentions_previous_pfam_name"],"pfam_id":"PF09369","protein_label":"","reviewed":null,"short_name":"MZB","source_category":"genomics","source_mech":"TraitMech","source_path":"data/traits/genomics/disarm_system.yaml","source_record_id":"traitmech:000211","source_record_label":"DISARM system","source_section":"record_text","taxon_id":"","taxon_label":"","uniprot_accession":"","unknown_status":"EX_DUF"}],"family":{"alphafold_models":5747,"candidate_reasons":["pfam_previous_unknown_name"],"characterization_status":"","context_evidence_count":null,"cross_mech":{"example_proteins":0,"protein_traits_record":false,"records_by_mech":{"TraitMech":1},"scanned":false},"curation_status":"SEEDED","demotion_reasons":[],"description":"This is the C-terminal MrfA Zn+2-binding domain (MZB, also referred to as DUF1998) which contains a conserved four-cysteine signature motif. These four Cys reside in a short coil between two alpha-helices and form a metal ion-binding site [[cite:PUB00098193]]. This domain is frequently found at the C-terminal of ndNTPases, however, it is also found encoded in a standalone gene, downstream of putative helicase domain-encoding genes associated with bacterial anti-phage defense system DISARM. MrfA (Mitomycin repair factor A, also known as YprA in Bacillus subtilis) is a DNA helicase that supports repair of mitomycin C-induced DNA damage. MrfA homologues are widely distributed in bacteria and are also present in archaea, fungi and plants. The MrfA-homologue in yeast, Hrq1, also reduces mitomycin C sensitivity. Hrq1 has high similarity to human RecQ4 and was therefore assigned to the RecQ-like helicase family. MrfA homologues appear to be missing in Enterobacteria, however, certain pathogenic Escherichia coli and Salmonella strains harbour Z5898-like helicases with this domain [[cite:PUB00098193]].","domain_architectures":99,"interpro_id":"IPR018973","known_evidence_count":null,"matches":11161,"name":"MrfA Zn-binding domain","partial_evidence_count":null,"pfam_id":"PF09369","proteins":11160,"proteomes":8342,"short_name":"MZB","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF09369","structures":5,"taxa":13990,"unknown_status":"EX_DUF"},"inputs":{"cross_mech":"cross-mech-duf-examples-2026-10-10","members":"pfam-uniprot-uniref90-2026-10-07","protein_examples":"duf-protein-examples-2026-10-13","worklist":"interpro-pfam-duf-2026-10-10"},"members":[],"metadata":{"curation_status":"SEEDED","local_source":"source/data/families/PF09369.yaml","record":{"characterization_status":"UNSCORED","counters":{"alphafold_models":5747,"domain_architectures":99,"matches":11161,"proteins":11160,"proteomes":8342,"structures":5,"taxa":13990},"curation_status":"SEEDED","description":"This is the C-terminal MrfA Zn+2-binding domain (MZB, also referred to as DUF1998) which contains a conserved four-cysteine signature motif. These four Cys reside in a short coil between two alpha-helices and form a metal ion-binding site [[cite:PUB00098193]]. This domain is frequently found at the C-terminal of ndNTPases, however, it is also found encoded in a standalone gene, downstream of putative helicase domain-encoding genes associated with bacterial anti-phage defense system DISARM. MrfA (Mitomycin repair factor A, also known as YprA in Bacillus subtilis) is a DNA helicase that supports repair of mitomycin C-induced DNA damage. MrfA homologues are widely distributed in bacteria and are also present in archaea, fungi and plants. The MrfA-homologue in yeast, Hrq1, also reduces mitomycin C sensitivity. Hrq1 has high similarity to human RecQ4 and was therefore assigned to the RecQ-like helicase family. MrfA homologues appear to be missing in Enterobacteria, however, certain pathogenic Escherichia coli and Salmonella strains harbour Z5898-like helicases with this domain [[cite:PUB00098193]].","id":"Pfam:PF09369","interpro_id":"InterPro:IPR018973","name":"MrfA Zn-binding 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