{"cross_mech":[],"family":{"alphafold_models":2686,"candidate_reasons":["pfam_previous_unknown_name"],"characterization_status":"","context_evidence_count":null,"cross_mech":{"example_proteins":0,"protein_traits_record":false,"records_by_mech":{},"scanned":false},"curation_status":"SEEDED","demotion_reasons":[],"description":"This is the conserved leucine-rich domain (LRD) within the C-terminal domain of SPIN90 (also known as NCK interacting protein with SH3 domain (NCKIPSD)) from animals, Ldb17 from budding yeasts and Dip1 from fission yeasts [[cite:PUB00098231]]. Ldb17 is involved in the regulation of endocytosis in yeast [[cite:PUB00054166]]. SPIN90 is an F-actin binding protein that regulates actin polymerization and endocytosis [[cite:PUB00085468]]. It associates with and activates the Arp2/3 complex, without requiring a preformed actin filament [[cite:PUB00054166],[cite:PUB00033588],[cite:PUB00054167]]. This LRD, within the C-terminal armadillo repeat (ARM) domain of SPIN90, is important for activation of Arp2/3 complex [[cite:PUB00098231]].","domain_architectures":55,"interpro_id":"IPR018556","known_evidence_count":null,"matches":3169,"name":"SPIN90/Ldb17, leucine-rich domain","partial_evidence_count":null,"pfam_id":"PF09431","proteins":3159,"proteomes":2626,"short_name":"SPIN90_LRD","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF09431","structures":7,"taxa":6971,"unknown_status":"EX_DUF"},"inputs":{"cross_mech":"cross-mech-duf-examples-2026-10-10","members":"pfam-uniprot-uniref90-2026-10-07","protein_examples":"duf-protein-examples-2026-10-13","worklist":"interpro-pfam-duf-2026-10-10"},"members":[],"metadata":{"curation_status":"SEEDED","local_source":"source/data/families/PF09431.yaml","record":{"characterization_status":"UNSCORED","counters":{"alphafold_models":2686,"domain_architectures":55,"matches":3169,"proteins":3159,"proteomes":2626,"structures":7,"taxa":6971},"curation_status":"SEEDED","description":"This is the conserved leucine-rich domain (LRD) within the C-terminal domain of SPIN90 (also known as NCK interacting protein with SH3 domain (NCKIPSD)) from animals, Ldb17 from budding yeasts and Dip1 from fission yeasts [[cite:PUB00098231]]. Ldb17 is involved in the regulation of endocytosis in yeast [[cite:PUB00054166]]. SPIN90 is an F-actin binding protein that regulates actin polymerization and endocytosis [[cite:PUB00085468]]. It associates with and activates the Arp2/3 complex, without requiring a preformed actin filament [[cite:PUB00054166],[cite:PUB00033588],[cite:PUB00054167]]. This LRD, within the C-terminal armadillo repeat (ARM) domain of SPIN90, is important for activation of Arp2/3 complex [[cite:PUB00098231]].","id":"Pfam:PF09431","interpro_id":"InterPro:IPR018556","name":"SPIN90/Ldb17, leucine-rich domain","pfam_id":"PF09431","provenance":{"generated_at":"2026-10-10T07:44:21.614711Z","path":"data/worklists/interpro-pfam-duf-2026-10-10.json","sha256":"17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383","snapshot_id":"interpro-pfam-duf-2026-10-10","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/"},"seed_status":"EX_DUF","short_name":"SPIN90_LRD","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF09431"},"source":{"commit":"c8155abfcf881ad8662936343deb81e604e3a194","path":"data/families/PF09431.yaml","repository":"https://github.com/CultureBotAI/DUFMech","sha256":"e3b8439f0f4652ab44e629fb796c7ab8607d1a8fe20da5426b374f09c1ebbcb7"}},"protein_examples":{"candidate_count":3,"discovery_scanned":true,"example_count":3,"examples":[{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Saccharomyces cerevisiae (strain ATCC 204508 / S288c)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/interpro/spin90-ldb17-leucine-rich-domain-ipr018556.yaml","qualification_status":"NOT_ASSESSED","record_id":"InterPro:IPR018556","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/interpro/spin90-ldb17-leucine-rich-domain-ipr018556.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/pfam/spin90-lrd-pf09431.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF09431","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/pfam/spin90-lrd-pf09431.yaml"}],"pfam_ids":["PF09431"],"protein_name":"Protein LDB17","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MILDPLSPNIENHTQDEIIEFWEKTESIANIPKENLDESHVNSSLVAYLKFATDSYKVFINTDRDLYRMSLILLESSLFEFKKEFCLSKLQSLLNIDLLEMNMKFIIVYILLCEAKKNVYSLEIMLKFQGFTVFYNTLYTQFAYLSKYGKERTVASKHQYNSNNSSTGTSLDSLDRSLTDIDLGIIDEMKQISTVLMDLLFQIMKYCKCVIANLQIVDDFFVYYMMESMRSDTMDDMFNNAEFKLLLALNEQYMMFAKEYDIENKVYKYLINGSVSRCFTELLLLKFNRASDPPLQIMMCKIIYLILTPRGDYSPMNFFYTNDLRVLIDVLIRELQNISEDEEVLRNTLLRVLIPLLKNTQLSKTHYRKDDLNKLLNYLSTLDNICVDSPALHEHQVTVALSRKCLQQIPWLETPSTPSDGGSSVSSNNTSRNSSIVALGTPDNQNILARKGHLYSNRELDVSAESLTKRKAKAPPPPPPPPPSRKCGTPK","sequence_length":491,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"c87ba5d94cdb6e81389076946ffe24d9e1e8625e8b8e92e74d5f83ce61d69a48","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/Q12342.json","taxon_id":"559292","uniprot_accession":"Q12342","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Mus musculus","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/cdd/src-homology-3-domain-of-sh3-protein-interacting-with-nck-90-kda-spin9-cd11849.yaml","qualification_status":"NOT_ASSESSED","record_id":"CDD:cd11849","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/cdd/src-homology-3-domain-of-sh3-protein-interacting-with-nck-90-kda-spin9-cd11849.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/interpro/spin90-ldb17-leucine-rich-domain-ipr018556.yaml","qualification_status":"NOT_ASSESSED","record_id":"InterPro:IPR018556","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/interpro/spin90-ldb17-leucine-rich-domain-ipr018556.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/interpro/spin90-sh3-domain-ipr035514.yaml","qualification_status":"NOT_ASSESSED","record_id":"InterPro:IPR035514","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/interpro/spin90-sh3-domain-ipr035514.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/pfam/spin90-lrd-pf09431.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF09431","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/pfam/spin90-lrd-pf09431.yaml"}],"pfam_ids":["PF00018","PF09431"],"protein_name":"NCK-interacting protein with SH3 domain","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MYRALYAFRSAEPNAMAFAAGETFLVLERSSTHWWLAARARSGETGYVPPAYLHRLQGMEQDVLQAIDRAIEAVHNTAMRDGGKYSLEQRGVLQKLIHHRKETLSRRGTSASSATVMTPSTSDHHLDAAVSRQPNGVCRTGFERQHSLPSSEHLGTDGALYQVPPQPRRAAPTTPPPPVKRRDREALVISGSGGRTAIPSGGSSVSSGSSASSTSMDTLYTGSSPSELGPSCSPTPPPVPRRGAHTTVSQPQPSPSKAPSPEPPTEEVAAETNSTPDDLEAQDALSPETTEEKAAAETVVPRTIGAELMELVRRNTGLSHELCRVAIGVVVGHIQATVPASSPIMEQVLLSLVEGKDLSTALPSGQVCHDQQRLEVIFADLARRKDDAQQRSWALYEDEDVIRCYLEELLHILTDADPEVCKKMCKRSDFESVLALVAYYQMEHRASLRLLLLKCFGAMCSLDAAIISTLVSSVLPVELARDMQTDTQDHQKLCYSALVLAMVFSMGEAVPYAHYEHLGTPFAQFLLSIVEDGLPMDTTEQLPDLCMNLLLALNLHLTAPEQNVIMAALSRHTNVKIFSEKLLLLLNRGDDPVRIFRHEPQPPHSVLKFLQDVFSSSATAAIFYHTDMMALIDITVRQIADLSPGDKLRMEYLSLMHAVVRSTPYLQHRHRLSDLQATLRRILTEEEASPQCQMDRMIVQEMYKEFPDLGEVPS","sequence_length":714,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"9412b267704190bfeeedffaf535ba50478ab78e26effed7b25f7303b77c03d29","sequence_version":2,"source_url":"https://rest.uniprot.org/uniprotkb/Q9ESJ4.json","taxon_id":"10090","uniprot_accession":"Q9ESJ4","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Homo sapiens","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/cdd/src-homology-3-domain-of-sh3-protein-interacting-with-nck-90-kda-spin9-cd11849.yaml","qualification_status":"NOT_ASSESSED","record_id":"CDD:cd11849","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/cdd/src-homology-3-domain-of-sh3-protein-interacting-with-nck-90-kda-spin9-cd11849.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/interpro/spin90-ldb17-leucine-rich-domain-ipr018556.yaml","qualification_status":"NOT_ASSESSED","record_id":"InterPro:IPR018556","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/interpro/spin90-ldb17-leucine-rich-domain-ipr018556.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/interpro/spin90-sh3-domain-ipr035514.yaml","qualification_status":"NOT_ASSESSED","record_id":"InterPro:IPR035514","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/interpro/spin90-sh3-domain-ipr035514.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/pfam/spin90-lrd-pf09431.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF09431","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/pfam/spin90-lrd-pf09431.yaml"}],"pfam_ids":["PF00018","PF09431"],"protein_name":"NCK-interacting protein with SH3 domain","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MYRALYAFRSAEPNALAFAAGETFLVLERSSAHWWLAARARSGETGYVPPAYLRRLQGLEQDVLQAIDRAIEAVHNTAMRDGGKYSLEQRGVLQKLIHHRKETLSRRGPSASSVAVMTSSTSDHHLDAAAARQPNGVCRAGFERQHSLPSSEHLGADGGLYQIPLPSSQIPPQPRRAAPTTPPPPVKRRDREALMASGSGGHNTMPSGGNSVSSGSSVSSTSLDTLYTSSSPSEPGSSCSPTPPPVPRRGTHTTVSQVQPPPSKASAPEPPAEEEVATGTTSASDDLEALGTLSLGTTEEKAAAEAAVPRTIGAELMELVRRNTGLSHELCRVAIGIIVGHIQASVPASSPVMEQVLLSLVEGKDLSMALPSGQVCHDQQRLEVIFADLARRKDDAQQRSWALYEDEGVIRCYLEELLHILTDADPEVCKKMCKRNEFESVLALVAYYQMEHRASLRLLLLKCFGAMCSLDAAIISTLVSSVLPVELARDMQTDTQDHQKLCYSALILAMVFSMGEAVPYAHYEHLGTPFAQFLLNIVEDGLPLDTTEQLPDLCVNLLLALNLHLPAADQNVIMAALSKHANVKIFSEKLLLLLNRGDDPVRIFKHEPQPPHSVLKFLQDVFGSPATAAIFYHTDMMALIDITVRHIADLSPGDKLRMEYLSLMHAIVRTTPYLQHRHRLPDLQAILRRILNEEETSPQCQMDRMIVREMCKEFLVLGEAPS","sequence_length":722,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"e849a8eefe3df1749af42a30527148648da5aea030f98302ba415e693800466c","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/Q9NZQ3.json","taxon_id":"9606","uniprot_accession":"Q9NZQ3","uniprot_release":"2026_03"}],"pfam_id":"PF09431","short_name":"SPIN90_LRD","status":"HAS_SEQUENCE_EXAMPLE","unresolved":[]}}