{"cross_mech":[],"family":{"alphafold_models":2061,"candidate_reasons":["pfam_previous_unknown_name"],"characterization_status":"","context_evidence_count":null,"cross_mech":{"example_proteins":0,"protein_traits_record":false,"records_by_mech":{},"scanned":false},"curation_status":"SEEDED","demotion_reasons":[],"description":"HigB_toxin is a family of RelE-like prokaryotic proteins that function as mRNA interferases. HigB cleaves translated mRNA only, and cleavage depended on translation of the target RNAs. HigB belongs to the RelE super-family of RNases. The toxin-antitoxin gene-pair is induced by environmental stress factors.","domain_architectures":5,"interpro_id":"IPR018669","known_evidence_count":null,"matches":3200,"name":"HigB_toxin, RelE-like toxic component of a toxin-antitoxin system","partial_evidence_count":null,"pfam_id":"PF09907","proteins":3199,"proteomes":2308,"short_name":"HigB_toxin","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF09907","structures":4,"taxa":4015,"unknown_status":"EX_DUF"},"inputs":{"cross_mech":"cross-mech-duf-examples-2026-10-10","members":"pfam-uniprot-uniref90-2026-10-07","protein_examples":"duf-protein-examples-2026-10-13","worklist":"interpro-pfam-duf-2026-10-10"},"members":[],"metadata":{"curation_status":"SEEDED","local_source":"source/data/families/PF09907.yaml","record":{"characterization_status":"UNSCORED","counters":{"alphafold_models":2061,"domain_architectures":5,"matches":3200,"proteins":3199,"proteomes":2308,"structures":4,"taxa":4015},"curation_status":"SEEDED","description":"HigB_toxin is a family of RelE-like prokaryotic proteins that function as mRNA interferases. HigB cleaves translated mRNA only, and cleavage depended on translation of the target RNAs. HigB belongs to the RelE super-family of RNases. The toxin-antitoxin gene-pair is induced by environmental stress factors.","id":"Pfam:PF09907","interpro_id":"InterPro:IPR018669","name":"HigB_toxin, RelE-like toxic component of a toxin-antitoxin system","pfam_id":"PF09907","provenance":{"generated_at":"2026-10-10T07:44:21.614711Z","path":"data/worklists/interpro-pfam-duf-2026-10-10.json","sha256":"17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383","snapshot_id":"interpro-pfam-duf-2026-10-10","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/"},"seed_status":"EX_DUF","short_name":"HigB_toxin","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF09907"},"source":{"commit":"c8155abfcf881ad8662936343deb81e604e3a194","path":"data/families/PF09907.yaml","repository":"https://github.com/CultureBotAI/DUFMech","sha256":"e1c7ce6a56c79763f49df4f970dcccd2b89fc864b07b3109af905b3e1068e8b7"}},"protein_examples":{"candidate_count":3,"discovery_scanned":true,"example_count":3,"examples":[{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Escherichia coli (strain K12)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/localization/go/toxin-antitoxin-complex-go0110001.yaml","qualification_status":"NOT_ASSESSED","record_id":"GO:0110001","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/localization/go/toxin-antitoxin-complex-go0110001.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/family/pfam/higb-toxin-pf09907.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF09907","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/family/pfam/higb-toxin-pf09907.yaml"}],"pfam_ids":["PF09907"],"protein_name":"mRNA interferase toxin HigB","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKGKK","sequence_length":104,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"2a9800a3837c020ac74e044d5dd2dbe047681398acfdd8e7b4bc6e7c35155700","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/P64578.json","taxon_id":"83333","uniprot_accession":"P64578","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Shigella flexneri","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/family/pfam/higb-toxin-pf09907.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF09907","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/family/pfam/higb-toxin-pf09907.yaml"}],"pfam_ids":["PF09907"],"protein_name":"mRNA interferase HigB","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKGKK","sequence_length":104,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"2a9800a3837c020ac74e044d5dd2dbe047681398acfdd8e7b4bc6e7c35155700","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/P64580.json","taxon_id":"623","uniprot_accession":"P64580","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/family/pfam/higb-toxin-pf09907.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF09907","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/family/pfam/higb-toxin-pf09907.yaml"}],"pfam_ids":["PF09907"],"protein_name":"mRNA interferase HigB","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENKE","sequence_length":103,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"9889cfd6a4f28f8160aa482b55fd51685edece41dba402529ca147c59c46a300","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/Q8FEM8.json","taxon_id":"199310","uniprot_accession":"Q8FEM8","uniprot_release":"2026_03"}],"pfam_id":"PF09907","short_name":"HigB_toxin","status":"HAS_SEQUENCE_EXAMPLE","unresolved":[]}}