{"cross_mech":[],"family":{"alphafold_models":1101,"candidate_reasons":["pfam_previous_unknown_name"],"characterization_status":"","context_evidence_count":null,"cross_mech":{"example_proteins":0,"protein_traits_record":false,"records_by_mech":{},"scanned":false},"curation_status":"SEEDED","demotion_reasons":[],"description":"This entry includes family members such as EarP enzymes which are essential for post-translational activation of elongation factor P(EF-P). It was identified as EF-P arginine R32 specific rhamnosyl transferase in Shewanella oneidensis using dTDP-beta-L-rhamnose as donor substrate [[cite:PUB00091040]]. This was further confirmed for Pseudomonas aeruginosa [[cite:PUB00091041]], Pseudomonas putida [[cite:PUB00091042]] and Neisseria meningitidis [[cite:PUB00091043]]. As for S. oneidensis [[cite:PUB00091044]] and P. aeruginosa [[cite:PUB00091045]], EarP enzyme acts as an inverting glycosyltransferase, thus mediating the formation of an alpha-L-rhamnosidic linkage. Structural analysis show that EarP is composed of two opposing domains with Rossmann folds, thus constituting a B pattern-type glycosyltransferase (GT-B) and provide basis for arginine glycosylation by EarP [[cite:PUB00091042]]. Mutational analysis of efp and earP genes, resulted in a substantial decrease in the production of rhamnolipids and pyocyanin (important factors for colonization and invasion during infection) of P. aeruginosa. Collectively this indicates that EarP and EF-P are essential for P. aeruginosa pathogenicity [[cite:PUB00091040]].The protein family is also annotated in the CaZy Database as GT104.","domain_architectures":4,"interpro_id":"IPR016633","known_evidence_count":null,"matches":1898,"name":"Elongation-Factor P (EF-P) rhamnosyltransferase EarP","partial_evidence_count":null,"pfam_id":"PF10093","proteins":1864,"proteomes":1846,"short_name":"EarP","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF10093","structures":12,"taxa":2406,"unknown_status":"EX_DUF"},"inputs":{"cross_mech":"cross-mech-duf-examples-2026-10-10","members":"pfam-uniprot-uniref90-2026-10-07","protein_examples":"duf-protein-examples-2026-10-13","worklist":"interpro-pfam-duf-2026-10-10"},"members":[],"metadata":{"curation_status":"SEEDED","local_source":"source/data/families/PF10093.yaml","record":{"characterization_status":"UNSCORED","counters":{"alphafold_models":1101,"domain_architectures":4,"matches":1898,"proteins":1864,"proteomes":1846,"structures":12,"taxa":2406},"curation_status":"SEEDED","description":"This entry includes family members such as EarP enzymes which are essential for post-translational activation of elongation factor P(EF-P). It was identified as EF-P arginine R32 specific rhamnosyl transferase in Shewanella oneidensis using dTDP-beta-L-rhamnose as donor substrate [[cite:PUB00091040]]. This was further confirmed for Pseudomonas aeruginosa [[cite:PUB00091041]], Pseudomonas putida [[cite:PUB00091042]] and Neisseria meningitidis [[cite:PUB00091043]]. As for S. oneidensis [[cite:PUB00091044]] and P. aeruginosa [[cite:PUB00091045]], EarP enzyme acts as an inverting glycosyltransferase, thus mediating the formation of an alpha-L-rhamnosidic linkage. Structural analysis show that EarP is composed of two opposing domains with Rossmann folds, thus constituting a B pattern-type glycosyltransferase (GT-B) and provide basis for arginine glycosylation by EarP [[cite:PUB00091042]]. Mutational analysis of efp and earP genes, resulted in a substantial decrease in the production of rhamnolipids and pyocyanin (important factors for colonization and invasion during infection) of P. aeruginosa. Collectively this indicates that EarP and EF-P are essential for P. aeruginosa pathogenicity [[cite:PUB00091040]].The protein family is also annotated in the CaZy Database as GT104.","id":"Pfam:PF10093","interpro_id":"InterPro:IPR016633","name":"Elongation-Factor P (EF-P) rhamnosyltransferase EarP","pfam_id":"PF10093","provenance":{"generated_at":"2026-10-10T07:44:21.614711Z","path":"data/worklists/interpro-pfam-duf-2026-10-10.json","sha256":"17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383","snapshot_id":"interpro-pfam-duf-2026-10-10","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/"},"seed_status":"EX_DUF","short_name":"EarP","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF10093"},"source":{"commit":"c8155abfcf881ad8662936343deb81e604e3a194","path":"data/families/PF10093.yaml","repository":"https://github.com/CultureBotAI/DUFMech","sha256":"1c34d8e2cc737fc6bd20b713ef82624f89b374d44a0428023281d7f08c7110fd"}},"protein_examples":{"candidate_count":3,"discovery_scanned":true,"example_count":3,"examples":[{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Neisseria meningitidis serogroup B / serotype 15 (strain H44/76)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/family/pfam/earp-pf10093.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF10093","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/family/pfam/earp-pf10093.yaml"}],"pfam_ids":["PF10093"],"protein_name":"Protein-arginine rhamnosyltransferase","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MNTPPFVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWTDDVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTAPVPDVVIETFACDLPENVLHIIRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYCEAVRFDTEALRERLMLPEKNASEWLLFGYRSDVWAKWLEMWRQAGSPMTLLLAGTQIIDSLKQSGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDQLLHLADCAVIRGEDSFVRAQLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYTPETVSAHRRLSDDLNGGEALSATQRLECWQTLQQHQNGWRQGAEDWSRYLFGQPSAPEKLAAFVSKHQKIR","sequence_length":382,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"e9b4ae3ade75e8f57df0d99216b29c7425796f32b335776b8d1afcee74bac084","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/E6MVV9.json","taxon_id":"909420","uniprot_accession":"E6MVV9","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/family/pfam/earp-pf10093.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF10093","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/family/pfam/earp-pf10093.yaml"}],"pfam_ids":["PF10093"],"protein_name":"Protein-arginine rhamnosyltransferase","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MKATWDIFCSVVDNYGDIGVTWRLARQLVAEHGLAVRLWVDDLNAFTPMCPGADATAAQQWQHGVDVRHWPAAWLPVAPADVVIGAFACQLPAAYVEAMRARPQPPLWLNLEYLSAEDWVEGCHGLPSPQPNGLRKVFFFPGFTDKTGGLLREGSLLARRDGFQQSAEARRAFLQGLGVDLVPGALLISLFAYENPQLGNWLDALATADQPCHLLVPQGRVVAGLSQWLGEGPLHVGDVRTRGALTVQVLPFVSQDDFDRLLWSCDFNAVRGEDSFVRAQWAGQPMLWHIYVQDENAHWEKLEAFLAHYRCGLSDDADAALLGLWRAWNMDFDMGQAWRAARQHWPELQQHARLWGARQAAQPDLATALVHFYRNSL","sequence_length":377,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"1b3d6bf42e0e7f84175a702def01f93a21ba8aa594f5f9a611b50f461a26a8c6","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/Q88LS1.json","taxon_id":"160488","uniprot_accession":"Q88LS1","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/family/pfam/earp-pf10093.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF10093","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/family/pfam/earp-pf10093.yaml"}],"pfam_ids":["PF10093"],"protein_name":"Protein-arginine rhamnosyltransferase","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MASWDIFCSVVDNYGDIGVTWRLARQLAAEHGQAVRLWVDEPQAFARICPRADPVAHVQCLDGVEVRAWGRPWAPVAAADVVIEAFACELPEAHRQAMRERKRPSLWLNLEYLSAEEWIGSCHALPSLQACGLSKYFFFPGFREPSGGLLREAGLLERRRRFQASVSAQDEFLASLGVRRKVGERLISLFAYENPALPGWLEQLRDARQPSLLLVPEGRVLADVADWLRVATLAVGDVHVRDALRVQVLPFMAQDDYDRLLWCCDLNAVRGEDSFVRAQWAGRPLLWHIYRQEEETHLAKLEAFLELYCAGLPADLAENLRTFWLAWNAGGGLAGAWEGLERQLPEWRREAQRWADEQGMRPDLAARLVQFYADWL","sequence_length":376,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"f464e7819cc7ec9c2d1a31a77895892917ac7d5e04e905361ae49d1ed4c4d7d9","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/Q9HZZ1.json","taxon_id":"208964","uniprot_accession":"Q9HZZ1","uniprot_release":"2026_03"}],"pfam_id":"PF10093","short_name":"EarP","status":"HAS_SEQUENCE_EXAMPLE","unresolved":[]}}