{"cross_mech":[],"family":{"alphafold_models":2,"candidate_reasons":["pfam_previous_unknown_name"],"characterization_status":"","context_evidence_count":null,"cross_mech":{"example_proteins":0,"protein_traits_record":false,"records_by_mech":{},"scanned":false},"curation_status":"SEEDED","demotion_reasons":[],"description":"This entry represents protein 3a encoded by Orf3/3a, also known as X1, which forms homotetrameric potassium, sodium or calcium sensitive ion channels (viroporin) that causes ER-stress in host cells and may modulate virus release. This is a pro-apoptosis-inducing protein that localises to the endoplasmic reticulum (ER)-Golgi compartment. SARS-CoV causes apoptosis of infected cells through NLRP3 inflammasome activation [[cite:PUB00075660],[cite:PUB00075661],[cite:PUB00056032],[cite:PUB00075662]]. Protein 3a also promotes the ubiquitination of apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) mediated by its interaction with TNF receptor-associated factor 3 (TRAF3). This protein induces NF-kappa B activation and upregulates fibrinogen secretion with high cytokine production [[cite:PUB00094124]]. Through the activation of the PERK pathway of unfolded protein response (UPR), this protein causes phosphorylation of eIF2-alpha leading to reduced translation of cellular proteins and as the activation of pro-apoptotic downstream effectors (i.e ATF4, CHOP).","domain_architectures":1,"interpro_id":"IPR024407","known_evidence_count":null,"matches":78,"name":"Betacoronavirus viroporin","partial_evidence_count":null,"pfam_id":"PF11289","proteins":78,"proteomes":3,"short_name":"bCoV_viroporin","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF11289","structures":6,"taxa":58,"unknown_status":"EX_DUF"},"inputs":{"cross_mech":"cross-mech-duf-examples-2026-10-10","members":"pfam-uniprot-uniref90-2026-10-07","protein_examples":"duf-protein-examples-2026-10-13","worklist":"interpro-pfam-duf-2026-10-10"},"members":[],"metadata":{"curation_status":"SEEDED","local_source":"source/data/families/PF11289.yaml","record":{"characterization_status":"UNSCORED","counters":{"alphafold_models":2,"domain_architectures":1,"matches":78,"proteins":78,"proteomes":3,"structures":6,"taxa":58},"curation_status":"SEEDED","description":"This entry represents protein 3a encoded by Orf3/3a, also known as X1, which forms homotetrameric potassium, sodium or calcium sensitive ion channels (viroporin) that causes ER-stress in host cells and may modulate virus release. This is a pro-apoptosis-inducing protein that localises to the endoplasmic reticulum (ER)-Golgi compartment. SARS-CoV causes apoptosis of infected cells through NLRP3 inflammasome activation [[cite:PUB00075660],[cite:PUB00075661],[cite:PUB00056032],[cite:PUB00075662]]. Protein 3a also promotes the ubiquitination of apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) mediated by its interaction with TNF receptor-associated factor 3 (TRAF3). This protein induces NF-kappa B activation and upregulates fibrinogen secretion with high cytokine production [[cite:PUB00094124]]. Through the activation of the PERK pathway of unfolded protein response (UPR), this protein causes phosphorylation of eIF2-alpha leading to reduced translation of cellular proteins and as the activation of pro-apoptotic downstream effectors (i.e ATF4, CHOP).","id":"Pfam:PF11289","interpro_id":"InterPro:IPR024407","name":"Betacoronavirus viroporin","pfam_id":"PF11289","provenance":{"generated_at":"2026-10-10T07:44:21.614711Z","path":"data/worklists/interpro-pfam-duf-2026-10-10.json","sha256":"17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383","snapshot_id":"interpro-pfam-duf-2026-10-10","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/"},"seed_status":"EX_DUF","short_name":"bCoV_viroporin","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF11289"},"source":{"commit":"c8155abfcf881ad8662936343deb81e604e3a194","path":"data/families/PF11289.yaml","repository":"https://github.com/CultureBotAI/DUFMech","sha256":"286c737ee988d8fe0edad61a187e58b6a90f9c041143dd3b4d485476fdd70886"}},"protein_examples":{"candidate_count":3,"discovery_scanned":true,"example_count":3,"examples":[{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Severe acute respiratory syndrome coronavirus 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protein","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MDLFMRIFTIGTVTLKQGEIKDATPSDFVRATATIPIQASLPFGWLIVGVALLAVFQSASKIITLKKRWQLALSKGVHFVCNLLLLFVTVYSHLLLVAAGLEAPFLYLYALVYFLQSINFVRIIMRLWLCWKCRSKNPLLYDANYFLCWHTNCYDYCIPYNSVTSSIVITSGDGTTSPISEHDYQIGGYTEKWESGVKDCVVLHSYFTSDYYQLYSTQLSTDTGVEHVTFFIYNKIVDEPEEHVQIHTIDGSSGVVNPVMEPIYDEPTTTTSVPL","sequence_length":275,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"73041fbba65b27c6e02b3af70d86be278edbbc084c5aa01067f7cc30790a4114","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/P0DTC3.json","taxon_id":"2697049","uniprot_accession":"P0DTC3","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Severe acute respiratory syndrome 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