{"cross_mech":[],"family":{"alphafold_models":1099,"candidate_reasons":["pfam_previous_unknown_name"],"characterization_status":"","context_evidence_count":null,"cross_mech":{"example_proteins":0,"protein_traits_record":false,"records_by_mech":{},"scanned":false},"curation_status":"SEEDED","demotion_reasons":[],"description":"This entry represents the uncharacterised C-terminal domain of secreted (or membrane-anchored) pullulanases of Gram-negative bacteria and pullulanase-type starch debranching enzymes of plants [[cite:PUB00097791]]. Both enzymes hydrolyse alpha-1,6 glycosidic linkages. Pullulan is an unusual, industrially important polysaccharide in which short alpha-1,4 chains (maltotriose) are connected in alpha-1,6 linkages. Enzymes that cleave alpha-1,6 linkages in pullulan and release maltotriose are called pullulanases although pullulan itself may not be the natural substrate.","domain_architectures":143,"interpro_id":"IPR024561","known_evidence_count":null,"matches":3256,"name":"Alpha-1,6-glucosidases, pullulanase-type, C-terminal","partial_evidence_count":null,"pfam_id":"PF11852","proteins":3239,"proteomes":2756,"short_name":"Pullul_strch_C","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF11852","structures":28,"taxa":4152,"unknown_status":"EX_DUF"},"inputs":{"cross_mech":"cross-mech-duf-examples-2026-10-10","members":"pfam-uniprot-uniref90-2026-10-07","protein_examples":"duf-protein-examples-2026-10-13","worklist":"interpro-pfam-duf-2026-10-10"},"members":[],"metadata":{"curation_status":"SEEDED","local_source":"source/data/families/PF11852.yaml","record":{"characterization_status":"UNSCORED","counters":{"alphafold_models":1099,"domain_architectures":143,"matches":3256,"proteins":3239,"proteomes":2756,"structures":28,"taxa":4152},"curation_status":"SEEDED","description":"This entry represents the uncharacterised C-terminal domain of secreted (or membrane-anchored) pullulanases of Gram-negative bacteria and pullulanase-type starch debranching enzymes of plants [[cite:PUB00097791]]. Both enzymes hydrolyse alpha-1,6 glycosidic linkages. Pullulan is an unusual, industrially important polysaccharide in which short alpha-1,4 chains (maltotriose) are connected in alpha-1,6 linkages. Enzymes that cleave alpha-1,6 linkages in pullulan and release maltotriose are called pullulanases although pullulan itself may not be the natural substrate.","id":"Pfam:PF11852","interpro_id":"InterPro:IPR024561","name":"Alpha-1,6-glucosidases, pullulanase-type, C-terminal","pfam_id":"PF11852","provenance":{"generated_at":"2026-10-10T07:44:21.614711Z","path":"data/worklists/interpro-pfam-duf-2026-10-10.json","sha256":"17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383","snapshot_id":"interpro-pfam-duf-2026-10-10","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/"},"seed_status":"EX_DUF","short_name":"Pullul_strch_C","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF11852"},"source":{"commit":"c8155abfcf881ad8662936343deb81e604e3a194","path":"data/families/PF11852.yaml","repository":"https://github.com/CultureBotAI/DUFMech","sha256":"7b04b272b40e7735cb8f1abaa72eda9f320b8b5fbc5f6771ee1e1cb7a4ffde97"}},"protein_examples":{"candidate_count":1,"discovery_scanned":true,"example_count":1,"examples":[{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Arabidopsis thaliana","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/family/pfam/pullul-strch-c-pf11852.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF11852","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/family/pfam/pullul-strch-c-pf11852.yaml"}],"pfam_ids":["PF02922","PF11852","PF17967"],"protein_name":"Pullulanase 1, chloroplastic","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MALTLTPTSSVHLLSSISVARPRIFAADFNLRSRWRRRRPVTSISNFRLRLPSKTSLHCLCSSSSASSPMSLEVSSPNSQFLDCLIYSRAYWVTQGVIAWNVDVGEGSCYFYASKSAGLSFSEDGIDGYDLRIKLEAESGSLPADVIEKFPHIRNYKSFKVPKDLDIRDLVKSQLAVVCFDAEGRLIQGTGLQLPGVLDELFSYDGPLGAHFTPEGVSLHLWAPTAQAVSVCIYKNPLDKSPMEICPLKEANGVWSTEGACSWGGCYYVYKVSVYHPSTMKLETCYANDPYARGLSADGRKTFLVNLDSDDLKPEGWDNLADKKPCLRSFSDISIYELHVRDFSANDETVEPENRGGYLAFTSKDSAGVKHLQKLVDAGLTHLHLLPTFQFGDVDDEKENWKSVDTSLLEGLRPDSTEAQARITEIQNDDGYNWGYNPVLWGVPKGSYASDPTGPCRIIEFRKMVQALNCTGLNVVLDVVYNHLHASGPHDKESVLDKIVPGYYLRRNSDGFIENSTCVNNTASEHYMVDRLIRDDLLNWVVNYKVDGFRFDLMGHIMKATIVNAKSAIGSLRKETDGVDGSRIYLYGEGWNFGEVAENGRGINASQFNLGGTGIGSFNDRIRDATLGGSPFGHPLQQGFITGLLLQPNAHDHGSEATQELMLSTAKNHIQTGMAANLKDYMLTNHEGKEVKGSEVLMHDATPVAYASLPTETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLASSMIALSQGIPFFHAGDEILRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKGKNEHNWPLIKPRLQDPSFKPKSSHIVATLHNFLDLLRIRYSSPLFRLDTARAIQERVRFHNTGPSSIPGAIVMSIEDGHRGIPSVSQIDPIYSLIVVIFNARPSEFSYPSPALKDRKLELHPVQVMSADEIVKKSVYDSFSGGFTVPARTTTVFVESRNG","sequence_length":965,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"f1abed568244e85bf5e5f2faf0aefdeeaed5e84b24a2a3c2aaa04ace2ebdf12d","sequence_version":2,"source_url":"https://rest.uniprot.org/uniprotkb/Q8GTR4.json","taxon_id":"3702","uniprot_accession":"Q8GTR4","uniprot_release":"2026_03"}],"pfam_id":"PF11852","short_name":"Pullul_strch_C","status":"HAS_SEQUENCE_EXAMPLE","unresolved":[]}}