{"cross_mech":[],"family":{"alphafold_models":13653,"candidate_reasons":["pfam_previous_unknown_name"],"characterization_status":"","context_evidence_count":null,"cross_mech":{"example_proteins":0,"protein_traits_record":false,"records_by_mech":{},"scanned":false},"curation_status":"SEEDED","demotion_reasons":[],"description":"This short domain is found at the C-terminus of many helicase proteins, including ATP-dependent rRNA helicase SPB4 from Saccharomyces cerevisiae, which is involved in the biogenesis of 60S ribosomal subunits. SPB4 is organised into four domains: dual RecA domains ([pfam:PF00270] and [pfam:PF00271]), a C-terminal extension (CTE, represented by this entry) and a C-terminal tail ([pfam:PF23681]). This domain packs closely onto the catalytic core and engages the four nucleotides immediately 5' to those within the RNA binding pocket. It shows a mainly alpha- helical structure [[cite:PUB00153742]].","domain_architectures":216,"interpro_id":"IPR025313","known_evidence_count":null,"matches":15992,"name":"ATP-dependent rRNA helicase SPB4-like, C-terminal extension","partial_evidence_count":null,"pfam_id":"PF13959","proteins":15982,"proteomes":3488,"short_name":"CTE_SPB4","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF13959","structures":50,"taxa":9181,"unknown_status":"EX_DUF"},"inputs":{"cross_mech":"cross-mech-duf-examples-2026-10-10","members":"pfam-uniprot-uniref90-2026-10-07","protein_examples":"duf-protein-examples-2026-10-13","worklist":"interpro-pfam-duf-2026-10-10"},"members":[],"metadata":{"curation_status":"SEEDED","local_source":"source/data/families/PF13959.yaml","record":{"characterization_status":"UNSCORED","counters":{"alphafold_models":13653,"domain_architectures":216,"matches":15992,"proteins":15982,"proteomes":3488,"structures":50,"taxa":9181},"curation_status":"SEEDED","description":"This short domain is found at the C-terminus of many helicase proteins, including ATP-dependent rRNA helicase SPB4 from Saccharomyces cerevisiae, which is involved in the biogenesis of 60S ribosomal subunits. SPB4 is organised into four domains: dual RecA domains ([pfam:PF00270] and [pfam:PF00271]), a C-terminal extension (CTE, represented by this entry) and a C-terminal tail ([pfam:PF23681]). This domain packs closely onto the catalytic core and engages the four nucleotides immediately 5' to those within the RNA binding pocket. It shows a mainly alpha- helical structure [[cite:PUB00153742]].","id":"Pfam:PF13959","interpro_id":"InterPro:IPR025313","name":"ATP-dependent rRNA helicase SPB4-like, C-terminal extension","pfam_id":"PF13959","provenance":{"generated_at":"2026-10-10T07:44:21.614711Z","path":"data/worklists/interpro-pfam-duf-2026-10-10.json","sha256":"17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383","snapshot_id":"interpro-pfam-duf-2026-10-10","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/"},"seed_status":"EX_DUF","short_name":"CTE_SPB4","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF13959"},"source":{"commit":"c8155abfcf881ad8662936343deb81e604e3a194","path":"data/families/PF13959.yaml","repository":"https://github.com/CultureBotAI/DUFMech","sha256":"25bde6cd0190890cb3c56c7baeaa82e45bbf0853fbc03e85914ee93f9c385c78"}},"protein_examples":{"candidate_count":6,"discovery_scanned":true,"example_count":6,"examples":[{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Aspergillus fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/pfam/ctt-spb4-pf23681.yaml","qualification_status":"NOT_ASSESSED","record_id":"Pfam:PF23681","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/pfam/ctt-spb4-pf23681.yaml"}],"pfam_ids":["PF00270","PF00271","PF13959","PF23681"],"protein_name":"ATP-dependent rRNA helicase spb4","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MAPKPPSGTSSRAWDAVTPALSEWVLEAMSSMGFTRMTPVQASAIPLFMAHKDVVVEAVTGSGKTLSFLIPVVEKLLRLEEPIKKHHIGAIIISPTRELASQIYNVLSSLLAFHPPSAAAINPSEDDDAPRPKFPSSTLKVVPQLLLGGSTTPAEDLSTFLKRSPNVLVSTPGRLLELLSSPHVHCPQSSFEMLVLDEADRLLDLGFKETLQNILRRLPKQRRTGLFSASVSEAVDQIVRVGLRNPVKVMVKVKGGSGVDDKRTPASLQMTYLTTPPSHKFAALKRILSSVQPTPLKTIFFVSTCSGVDYLSAILPLLLGDDFLLIPLHGKHQANVRQKNFNRFINSHDPAILLTTDVAARGLDIPSVDLVVQIDPPSDPKSFIHRCGRAGRAGRRGLSVVLLHPGREEDYVSFLEVRKTPVVPFSPLITFSDADAAAATATARKAVLADRALHDRGQKAFVSWFRSYSKHQASSIFRVSDLDWEALGKAWGLLKLPKMPELRNFTGDKTLGVSLDWNNYAYKDKQREKRRKELLQEAAESGATQSTSNKRRATESVAWSQQAESKNKKLKRREQKKSKHEKARWEKMTEEEKQKVLETEKMVEELRKKNEEERRLRRAAAKAAGAKADGDDEEEFQGFD","sequence_length":640,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"f81d44bc566f196bf5f58f07d69f810aec36e4a691922b324900268f31d541f9","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/A1D699.json","taxon_id":"331117","uniprot_accession":"A1D699","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Lodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/pfam/ctt-spb4-pf23681.yaml","qualification_status":"NOT_ASSESSED","record_id":"Pfam:PF23681","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/pfam/ctt-spb4-pf23681.yaml"}],"pfam_ids":["PF00270","PF00271","PF13959","PF23681"],"protein_name":"ATP-dependent rRNA helicase SPB4","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MQTNKGSLLWENLRVDLEPWLKDAIRSLNYPTMTPVQASTIPLLSGNKDVIVEAVTGSGKTLAFAIPVLQKVSKRLYQVPEGEEKPEPVKRGHMLAIVMAPTRELAKQIQMVFDKVLELLPEEDSYEPRIKTQLLVGFLGNVREDLDSYQENRPQILIATPGRLLDFMSLQIVKTSSLEIVILDEADKLLDMSFETDVIKILKMLPKQRRTGLFSATISAAGDTIFRTGMNNPVKLQVKTKNFLGEQNNAPTSLQLSYMMIEPEHKLTTMLQMLRDNQFKKAIVYFPTCTSVKHFYQMLSKLCKSSANDIDISALLFFSLHGQLTTKSRLNTLEKFTEGNDESKKYILMATDVAARGIDIPDVDLVIQIDPPTDPSVFLHRCGRTGRANKVGRAIVMLNNDTQEEDYVGFMEVKSVFMTKIDPPEDKDNHSFHNKFQKKLRKYMLEDRARHELAVKSYVGFVRYYSKHIASLIFRLASLDYIAIAKMYGLLRLPKMPESRYIENEKMPEDGWLGEVVDMDTYAYLDKSAEKARLENLEKDKLAKAENAKRRKELKVKNEAWSSKTEKRETKLERKEKMKRKREAIEKQLEAEQERGGLDEEEVKEDWKDLVRKNKKKQKSNGGGGGGGVLQGSFDDL","sequence_length":637,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"0d04a29bd820e5468e7e7ef426af50d33130611937546a69053d17d93fe62944","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/A5E2I8.json","taxon_id":"379508","uniprot_accession":"A5E2I8","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Saccharomyces cerevisiae (strain ATCC 204508 / S288c)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/pfam/ctt-spb4-pf23681.yaml","qualification_status":"NOT_ASSESSED","record_id":"Pfam:PF23681","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/pfam/ctt-spb4-pf23681.yaml"}],"pfam_ids":["PF00270","PF00271","PF13959","PF23681"],"protein_name":"ATP-dependent rRNA helicase SPB4","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MSKSLEWDNLGFSLLPWIRTGLDVMGFETMTPVQASTIPMLAGNKDVVVDSVTGSGKTAAFVIPVLEKVVKEEANTSKFKKAHFHSLIIAPTRELSRQIESVVLSFLEHYPSDLFPIKCQLLVGTNEATVRDDVSNFLRNRPQILIGTPGRVLDFLQMPAVKTSACSMVVMDEADRLLDMSFIKDTEKILRLLPKQRRTGLFSATMRSAGSDIFKTGLRNPVRITVNSKNQAPSSLKLNYCVVNPAEKLQLLVSILNNYKFKKCIVYFPTCVSVSYFYSFIQYLGKRNILVNEVEIFSLHGKLQTSARTKTLTAFTDSLSNSVLFTTDVAARGIDIPDVDLVIQLDPPTNTDMFMHRCGRTGRANRVGKAITFLNEGREEDFIPFMQVKNVELEELDLEVKGITTNFYEDFRNWILEDRDRFDKGVKAYVAFIKYYSNHSATSIFRLQSLDYVGIAKLYGLFRLPRMPEITKYLATEKQEGIFPGNWLVDPPVNMDEYKYKDKKREKERQETLKNISLINDKKKLKSELKKKNLAWSDKTLTKERKLERKEKMSLKRKAIEEELKAEELDENAEEERIKEDWKEIVLQNKRKKVSSKAIQGNFDDL","sequence_length":606,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"fe5a50bb0c0fcdb1955b733f1b8f85e0aecc4971787ef766be7277a7ab81a033","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/P25808.json","taxon_id":"559292","uniprot_accession":"P25808","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Homo sapiens","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/pfam/cte-spb4-pf13959.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF13959","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/pfam/cte-spb4-pf13959.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/pattern/dead-atp-helicase.yaml","qualification_status":"NOT_ASSESSED","record_id":"PROSITE:PS00039","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/pattern/dead-atp-helicase.yaml"}],"pfam_ids":["PF00270","PF00271","PF13959"],"protein_name":"Probable ATP-dependent RNA helicase DDX10","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MGKTANSPGSGARPDPVRSFNRWKKKHSHRQNKKKQLRKQLKKPEWQVERESISRLMQNYEKINVNEITRFSDFPLSKKTLKGLQEAQYRLVTEIQKQTIGLALQGKDVLGAAKTGSGKTLAFLVPVLEALYRLQWTSTDGLGVLIISPTRELAYQTFEVLRKVGKNHDFSAGLIIGGKDLKHEAERINNINILVCTPGRLLQHMDETVSFHATDLQMLVLDEADRILDMGFADTMNAVIENLPKKRQTLLFSATQTKSVKDLARLSLKNPEYVWVHEKAKYSTPATLEQNYIVCELQQKISVLYSFLRSHLKKKSIVFFSSCKEVQYLYRVFCRLRPGVSILALHGRQQQMRRMEVYNEFVRKRAAVLFATDIAARGLDFPAVNWVLQFDCPEDANTYIHRAGRTARYKEDGEALLILLPSEKAMVQQLLQKKVPVKEIKINPEKLIDVQKKLESILAQDQDLKERAQRCFVSYVRSVYLMKDKEVFDVSKLPIPEYALSLGLAVAPRVRFLQKMQKQPTKELVRSQADKVIEPRAPSLTNDEVEEFRAYFNEKMSILQKGGKRLEGTEHRQDNDTGNEEQEEEEDDEEEMEEKLAKAKGSQAPSLPNTSEAQKIKEVPTQFLDRDEEEEDADFLKVKRHNVFGLDLKDEKTLQKKEPSKSSIKKKMTKVAEAKKVMKRNFKVNKKITFTDEGELVQQWPQMQKSAIKDAEEDDDTGGINLHKAKERLQEEDKFDKEEYRKKIKAKHREKRLKEREARREANKRQAKAKDEEEAFLDWSDDDDDDDDGFDPSTLPDPDKYRSSEDSDSEDMENKISDTKKKQGMKKRSNSEVEDVGPTSHNRKKARWDTLEPLDTGLSLAEDEELVLHLLRSQS","sequence_length":875,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"d24eb73e2a96aee911c3c92148337fa5a8255b62c145edcd9d4c1727ac854713","sequence_version":2,"source_url":"https://rest.uniprot.org/uniprotkb/Q13206.json","taxon_id":"9606","uniprot_accession":"Q13206","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Homo sapiens","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/pfam/cte-spb4-pf13959.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF13959","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/pfam/cte-spb4-pf13959.yaml"}],"pfam_ids":["PF00270","PF00271","PF13959"],"protein_name":"ATP-dependent RNA helicase DDX55","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MEHVTEGSWESLPVPLHPQVLGALRELGFPYMTPVQSATIPLFMRNKDVAAEAVTGSGKTLAFVIPILEILLRREEKLKKSQVGAIIITPTRELAIQIDEVLSHFTKHFPEFSQILWIGGRNPGEDVERFKQQGGNIIVATPGRLEDMFRRKAEGLDLASCVRSLDVLVLDEADRLLDMGFEASINTILEFLPKQRRTGLFSATQTQEVENLVRAGLRNPVRVSVKEKGVAASSAQKTPSRLENYYMVCKADEKFNQLVHFLRNHKQEKHLVFFSTCACVEYYGKALEVLVKGVKIMCIHGKMKYKRNKIFMEFRKLQSGILVCTDVMARGIDIPEVNWVLQYDPPSNASAFVHRCGRTARIGHGGSALVFLLPMEESYINFLAINQKCPLQEMKPQRNTADLLPKLKSMALADRAVFEKGMKAFVSYVQAYAKHECNLIFRLKDLDFASLARGFALLRMPKMPELRGKQFPDFVPVDVNTDTIPFKDKIREKQRQKLLEQQRREKTENEGRRKFIKNKAWSKQKAKKEKKKKMNEKRKREEGSDIEDEDMEELLNDTRLLKKLKKGKITEEEFEKGLLTTGKRTIKTVDLGISDLEDDC","sequence_length":600,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"46442fe990309a7ae743099a011abc55c1aa332d19da4659d9174d14dbe45ba4","sequence_version":3,"source_url":"https://rest.uniprot.org/uniprotkb/Q8NHQ9.json","taxon_id":"9606","uniprot_accession":"Q8NHQ9","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Homo sapiens","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/pfam/cte-spb4-pf13959.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF13959","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/pfam/cte-spb4-pf13959.yaml"}],"pfam_ids":["PF00270","PF00271","PF13959"],"protein_name":"ATP-dependent DNA helicase DDX31","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MAPDLASQRHSESFPSVNSRPNVILPGREGRREGLPPGGGTRGSLVPTRPVPPSPAPLGTSPYSWSRSGPGRGGGAGSSRVPRGVPGPAVCAPGSLLHHASPTQTMAAADGSLFDNPRTFSRRPPAQASRQAKATKRKYQASSEAPPAKRRNETSFLPAKKTSVKETQRTFKGNAQKMFSPKKHSVSTSDRNQEERQCIKTSSLFKNNPDIPELHRPVVKQVQEKVFTSAAFHELGLHPHLISTINTVLKMSSMTSVQKQSIPVLLEGRDALVRSQTGSGKTLAYCIPVVQSLQAMESKIQRSDGPYALVLVPTRELALQSFDTVQKLLKPFTWIVPGVLMGGEKRKSEKARLRKGINILISTPGRLVDHIKSTKNIHFSRLRWLVFDEADRILDLGFEKDITVILNAVNAECQKRQNVLLSATLTEGVTRLADISLHDPVSISVLDKSHDQLNPKDKAVQEVCPPPAGDKLDSFAIPESLKQHVTVVPSKLRLVCLAAFILQKCKFEEDQKMVVFFSSCELVEFHYSLFLQTLLSSSGAPASGQLPSASMRLKFLRLHGGMEQEERTAVFQEFSHSRRGVLLCTDVAARGLDLPQVTWIVQYNAPSSPAEYIHRIGRTARIGCHGSSLLILAPSEAEYVNSLASHKINVSEIKMEDILCVLTRDDCFKGKRWGAQKSHAVGPQEIRERATVLQTVFEDYVHSSERRVSWAKKALQSFIQAYATYPRELKHIFHVRSLHLGHVAKSFGLRDAPRNLSALTRKKRKAHVKRPDLHKKTQSKHSLAEILRSEYSSGMEADIAKVKKQNAPGEPGGRPLQHSLQPTPCFGRGKTLKWRKTQKGVQRDSKTSQKV","sequence_length":851,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"d83034a7f996dda9e821ad0875b3680e13d64ecb55f1321e11e9d728c92e9fe7","sequence_version":2,"source_url":"https://rest.uniprot.org/uniprotkb/Q9H8H2.json","taxon_id":"9606","uniprot_accession":"Q9H8H2","uniprot_release":"2026_03"}],"pfam_id":"PF13959","short_name":"CTE_SPB4","status":"HAS_SEQUENCE_EXAMPLE","unresolved":[]}}