{"cross_mech":[],"family":{"alphafold_models":14,"candidate_reasons":["pfam_previous_unknown_name"],"characterization_status":"","context_evidence_count":null,"cross_mech":{"example_proteins":0,"protein_traits_record":false,"records_by_mech":{},"scanned":false},"curation_status":"SEEDED","demotion_reasons":[],"description":"This entry represents PolF haem-oxygenase-like diiron oxidase which converts the desaturated l-isoleucine to polyoximic acid via an intramolecular C-N cyclization [[cite:PUB00164341]]. PolF exhibits dual functionality, orchestrating the sequential desaturation and cyclization with l-isoleucine as the initial substrate [[cite:PUB00164341]]. PolF folds into a seven-helix bundle, with three core helices coordinating the iron cofactor. Members of this family contain a highly conserved H-X3-E-X2-H sequence motif that completes the coordination of the diiron cofactors. A member belongs to a biosynthetic gene cluster (BGC). This BGC (BGC0000877) includes a member from the polyoxin A biosynthetic gene cluster from Streptomyces cacaoi subsp. asoensis [[cite:PUB00098985],[cite:PUB00098986]].","domain_architectures":1,"interpro_id":"IPR045776","known_evidence_count":null,"matches":31,"name":"PolF haem-oxygenase-like diiron oxidases","partial_evidence_count":null,"pfam_id":"PF19705","proteins":31,"proteomes":29,"short_name":"PolF","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF19705","structures":11,"taxa":62,"unknown_status":"EX_DUF"},"inputs":{"cross_mech":"cross-mech-duf-examples-2026-10-10","members":"pfam-uniprot-uniref90-2026-10-07","protein_examples":"duf-protein-examples-2026-10-13","worklist":"interpro-pfam-duf-2026-10-10"},"members":[],"metadata":{"curation_status":"SEEDED","local_source":"source/data/families/PF19705.yaml","record":{"characterization_status":"UNSCORED","counters":{"alphafold_models":14,"domain_architectures":1,"matches":31,"proteins":31,"proteomes":29,"structures":11,"taxa":62},"curation_status":"SEEDED","description":"This entry represents PolF haem-oxygenase-like diiron oxidase which converts the desaturated l-isoleucine to polyoximic acid via an intramolecular C-N cyclization [[cite:PUB00164341]]. PolF exhibits dual functionality, orchestrating the sequential desaturation and cyclization with l-isoleucine as the initial substrate [[cite:PUB00164341]]. PolF folds into a seven-helix bundle, with three core helices coordinating the iron cofactor. Members of this family contain a highly conserved H-X3-E-X2-H sequence motif that completes the coordination of the diiron cofactors. A member belongs to a biosynthetic gene cluster (BGC). This BGC (BGC0000877) includes a member from the polyoxin A biosynthetic gene cluster from Streptomyces cacaoi subsp. asoensis [[cite:PUB00098985],[cite:PUB00098986]].","id":"Pfam:PF19705","interpro_id":"InterPro:IPR045776","name":"PolF haem-oxygenase-like diiron oxidases","pfam_id":"PF19705","provenance":{"generated_at":"2026-10-10T07:44:21.614711Z","path":"data/worklists/interpro-pfam-duf-2026-10-10.json","sha256":"17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383","snapshot_id":"interpro-pfam-duf-2026-10-10","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/"},"seed_status":"EX_DUF","short_name":"PolF","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF19705"},"source":{"commit":"c8155abfcf881ad8662936343deb81e604e3a194","path":"data/families/PF19705.yaml","repository":"https://github.com/CultureBotAI/DUFMech","sha256":"3814d3be808dc8d6d65aae463da93badafe9e5c7d31f6880d393b487b864e0c1"}},"protein_examples":{"candidate_count":0,"discovery_scanned":true,"example_count":0,"examples":[],"pfam_id":"PF19705","short_name":"PolF","status":"NO_SIBLING_CANDIDATE","unresolved":[]}}