{"cross_mech":[],"family":{"alphafold_models":310,"candidate_reasons":["pfam_previous_unknown_name"],"characterization_status":"","context_evidence_count":null,"cross_mech":{"example_proteins":0,"protein_traits_record":false,"records_by_mech":{},"scanned":false},"curation_status":"SEEDED","demotion_reasons":[],"description":"Thoeris is a bacterial antiphage defense system, which consists of two genes, thsA and thsB, via NAD+ degradation [[cite:PUB00097806],[cite:PUB00101117],[cite:PUB00101118],[cite:PUB00160037]]. ThsA has robust NAD+ cleavage activity and a two-domain architecture containing an N-terminal NAD-binding domain (denoted as sirtuin-like or Macro) and C-terminal SLOG-like domain. In some instances, such as in B. amyloliquefaciens ThsA has an N-terminal transmembrane domain [[cite:PUB00097806]]. ThsB (also referred to as TIR1 and TIR2) is structurally similar to TIR domain proteins but without enzymatic activity. This domain is found at the C-terminal of ThsA proteins which adopts a Rossmann-like fold that binds NAD+/NAD+derivatives. It has been characterised as the SLOG-like domain which binds NAD+ derivatives such as ADPR molecules produced by ThsB, which drives an oligomeric state change in ThsA and activates the NADase activity (represented in [pfam:PF18185]) [[cite:PUB00097806],[cite:PUB00101118]]. This entry represents the NAD+-binding Macro domain from B. amyloliquefaciens [[cite:PUB00097806],[cite:PUB00101117]].","domain_architectures":9,"interpro_id":"IPR045535","known_evidence_count":null,"matches":534,"name":"Thoeris protein ThsA, Macro domain","partial_evidence_count":null,"pfam_id":"PF20016","proteins":534,"proteomes":453,"short_name":"ThsA_Macro","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF20016","structures":5,"taxa":1034,"unknown_status":"EX_DUF"},"inputs":{"cross_mech":"cross-mech-duf-examples-2026-10-10","members":"pfam-uniprot-uniref90-2026-10-07","protein_examples":"duf-protein-examples-2026-10-13","worklist":"interpro-pfam-duf-2026-10-10"},"members":[],"metadata":{"curation_status":"SEEDED","local_source":"source/data/families/PF20016.yaml","record":{"characterization_status":"UNSCORED","counters":{"alphafold_models":310,"domain_architectures":9,"matches":534,"proteins":534,"proteomes":453,"structures":5,"taxa":1034},"curation_status":"SEEDED","description":"Thoeris is a bacterial antiphage defense system, which consists of two genes, thsA and thsB, via NAD+ degradation [[cite:PUB00097806],[cite:PUB00101117],[cite:PUB00101118],[cite:PUB00160037]]. ThsA has robust NAD+ cleavage activity and a two-domain architecture containing an N-terminal NAD-binding domain (denoted as sirtuin-like or Macro) and C-terminal SLOG-like domain. In some instances, such as in B. amyloliquefaciens ThsA has an N-terminal transmembrane domain [[cite:PUB00097806]]. ThsB (also referred to as TIR1 and TIR2) is structurally similar to TIR domain proteins but without enzymatic activity. This domain is found at the C-terminal of ThsA proteins which adopts a Rossmann-like fold that binds NAD+/NAD+derivatives. It has been characterised as the SLOG-like domain which binds NAD+ derivatives such as ADPR molecules produced by ThsB, which drives an oligomeric state change in ThsA and activates the NADase activity (represented in [pfam:PF18185]) [[cite:PUB00097806],[cite:PUB00101118]]. This entry represents the NAD+-binding Macro domain from B. amyloliquefaciens [[cite:PUB00097806],[cite:PUB00101117]].","id":"Pfam:PF20016","interpro_id":"InterPro:IPR045535","name":"Thoeris protein ThsA, Macro domain","pfam_id":"PF20016","provenance":{"generated_at":"2026-10-10T07:44:21.614711Z","path":"data/worklists/interpro-pfam-duf-2026-10-10.json","sha256":"17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383","snapshot_id":"interpro-pfam-duf-2026-10-10","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/"},"seed_status":"EX_DUF","short_name":"ThsA_Macro","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF20016"},"source":{"commit":"c8155abfcf881ad8662936343deb81e604e3a194","path":"data/families/PF20016.yaml","repository":"https://github.com/CultureBotAI/DUFMech","sha256":"f028f4d4f209a913348e64f31e95cfbe226bdcdc3fb017b2aea76da240ae52d3"}},"protein_examples":{"candidate_count":0,"discovery_scanned":true,"example_count":0,"examples":[],"pfam_id":"PF20016","short_name":"ThsA_Macro","status":"NO_SIBLING_CANDIDATE","unresolved":[]}}