{"cross_mech":[],"family":{"alphafold_models":2159,"candidate_reasons":["pfam_previous_unknown_name"],"characterization_status":"","context_evidence_count":null,"cross_mech":{"example_proteins":0,"protein_traits_record":false,"records_by_mech":{},"scanned":false},"curation_status":"SEEDED","demotion_reasons":[],"description":"This entry represents a domain found at the C-terminal of bacterial proteins, including N,N-dimethylformamidase beta (DMFA2) subunit from Paracoccus [[cite:PUB00101281]], the large subunit of dimethylformamidase (DMFase), an enzyme containing a Fe+3 ion in the active site that catalyses the hydrolytic cleavage of the amide bond in N,N-dimethyl formamide. This domain is described as domain III, which adopts an alpha/beta/alpha fold, with five beta-strands that are parallel and sandwiched between alpha-helices. This domain is also found in some uncharacterised fungal sequences.","domain_architectures":234,"interpro_id":"IPR046540","known_evidence_count":null,"matches":4320,"name":"N,N-dimethylformamidase beta subunit-like, C-terminal","partial_evidence_count":null,"pfam_id":"PF20254","proteins":4303,"proteomes":2858,"short_name":"DMFA2_C","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF20254","structures":9,"taxa":4293,"unknown_status":"EX_DUF"},"inputs":{"cross_mech":"cross-mech-duf-examples-2026-10-10","members":"pfam-uniprot-uniref90-2026-10-07","protein_examples":"duf-protein-examples-2026-10-13","worklist":"interpro-pfam-duf-2026-10-10"},"members":[],"metadata":{"curation_status":"SEEDED","local_source":"source/data/families/PF20254.yaml","record":{"characterization_status":"UNSCORED","counters":{"alphafold_models":2159,"domain_architectures":234,"matches":4320,"proteins":4303,"proteomes":2858,"structures":9,"taxa":4293},"curation_status":"SEEDED","description":"This entry represents a domain found at the C-terminal of bacterial proteins, including N,N-dimethylformamidase beta (DMFA2) subunit from Paracoccus [[cite:PUB00101281]], the large subunit of dimethylformamidase (DMFase), an enzyme containing a Fe+3 ion in the active site that catalyses the hydrolytic cleavage of the amide bond in N,N-dimethyl formamide. This domain is described as domain III, which adopts an alpha/beta/alpha fold, with five beta-strands that are parallel and sandwiched between alpha-helices. This domain is also found in some uncharacterised fungal sequences.","id":"Pfam:PF20254","interpro_id":"InterPro:IPR046540","name":"N,N-dimethylformamidase beta subunit-like, C-terminal","pfam_id":"PF20254","provenance":{"generated_at":"2026-10-10T07:44:21.614711Z","path":"data/worklists/interpro-pfam-duf-2026-10-10.json","sha256":"17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383","snapshot_id":"interpro-pfam-duf-2026-10-10","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/"},"seed_status":"EX_DUF","short_name":"DMFA2_C","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF20254"},"source":{"commit":"c8155abfcf881ad8662936343deb81e604e3a194","path":"data/families/PF20254.yaml","repository":"https://github.com/CultureBotAI/DUFMech","sha256":"f9dd0a9f0e3bbeefa824e25a5e088707d5a56a1bb956d387d722c2f7adf4851e"}},"protein_examples":{"candidate_count":2,"discovery_scanned":true,"example_count":2,"examples":[{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Paracoccus aminophilus","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/pfam/dmfa2-c-pf20254.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF20254","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/pfam/dmfa2-c-pf20254.yaml"}],"pfam_ids":["PF20254"],"protein_name":"N,N-dimethylformamidase beta subunit","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MKTVKIRGYCDRLSAAPGETIRFYVSADTSDGSYEAELVRLIHGDTNPAGPGYKEESVKSAADGSYPARFQRTQFGSFVEVPDASGALLADGAFSVHTFLWSTTPGRGRQGLVSRWDDERQCGWSLAIEEGRLVFTIGDESGTTNRVISDRPLFQEVWYSVTAVFDPVQKTISLHQKSVVNRTNSRFGLVVPLDSDTTVSAVSQVSPGDSRTSLLIAGLGEAAAADGRTWCIANFNGKIDAPKLYGRALSSEEAMKLAEGTVAEPWSRLAHWDFSAGIGPDGIPTDHVVDISGNGHHGQCVNQPDRGSTGWNWDGHEENFIHCPQQYGALWFHEDCLDDCRWDKDFEITLPDGLKSDFYAMKIRYGDSEDYIPFFVLPPRGKATAKILVLASTFSYLAYANEQIMHKADIGQAVAGHTPVLNENDVELHRNLDYYGLSTYDGHVDGRGVQYTSWRRPILNLRPKHRQGFGSIWELPADLHLIDWLNHNGFDYDVATEHDLNEQGVDLLRRYNVVLTGSHPEYQTWANADAWEDYLADGGRGMYLAANGMYWIVSVHPEKPWVMEVRKELGVTAWEAPPGEYHYSTNGRRGGRFRGRARATQKIWGTGMSSFGFDHSGYFVQMPDSQDKRAAWIMDGIDPDERIGDGGLVGGGAGGYELDRYDLSLGTPPNTLLLASSVEHSVVYTVIPDDKSFPHPGMNGGEHPFVRADITYFSTANGGGMFSTSSISWLGSLSWNNYDNNVSRMTRNVLTQFMKDEPAPLV","sequence_length":762,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"2f028a2d8df249eb0425e0975e609b9aebc8dd9c9369b8ee349e39abec5c2ea3","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/C9DQ21.json","taxon_id":"34003","uniprot_accession":"C9DQ21","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Alcaligenes sp","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/pfam/dmfa2-c-pf20254.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF20254","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/pfam/dmfa2-c-pf20254.yaml"}],"pfam_ids":["PF07953","PF20254"],"protein_name":"N,N-dimethylformamidase beta subunit","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MKDIAIRGYCDRPSVATGETIRFYVSANETRGTFDAELVRLIHGDSNPAGPGYKEEAIKSDLEGQYPARFQRTQFGSYVEVADPDAGLQPDGAFSVHLFLWSTTPSRGRQGIASRWNDERQSGWNLAIEDGRVVFTIGDGSGATSSVVSDRPLFQQIWYSITGVYDPEKKQLRLYQKSVVNRTNSRFGLVVPLDSDCAVSADATVKAADSETSLLIAGLGEAAAQDGRTWCIAHYNGKVDAPKIYGCALGQDDAEKLSRGEIVRPISRLAHWDFSAGIGLNGIPTDHVVDASGNGHHGRCMNQPDRGSTGWNWDGHEENFIHCPEQYGALWFHEDCLDDCRWEKDFEFTVPEGLKSDFYAVKIRYEDTEDYIPFFVLPPRGTATAPILVIASTLSYLAYANEQIMHKADIGQAVAGHTPVLNENDVELHKNLSYYGLSTYDGHIDGRGVQYTSWRRPIMNLRPKHRQGFGSIWELPADLHLIDWLNHNGFEYDVATEHDLNDQGAELLRRYKVVLTGSHPEYQTWANADAWEDYLADGGRGMYLAANGMYWIVEVHPEKPWVMEVRKELGVTAWEAPPGEYHYSTNGRRGGRFRGRARATQKIWGTGMSSFGFDHSGYFVQMPDSQDERVAWIMEGIDPEERIGDGGLVGGGAGGYELDRYDLALGTPPNTLLLASSVEHSVVYTVIPDDKAFPHPGMNGGEHPFVRADITYFSTANGGGMFATSSISWLGSLSWNDYDNNVSKMTKNVLNQFIKDEPAPRV","sequence_length":762,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"5ebbc349c75e270f3f554ada6ecfd68322782bbbcaf711c54b9570621443edc4","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/Q9LCC0.json","taxon_id":"512","uniprot_accession":"Q9LCC0","uniprot_release":"2026_03"}],"pfam_id":"PF20254","short_name":"DMFA2_C","status":"HAS_SEQUENCE_EXAMPLE","unresolved":[]}}