{"cross_mech":[],"family":{"alphafold_models":1582,"candidate_reasons":["pfam_previous_unknown_name"],"characterization_status":"","context_evidence_count":null,"cross_mech":{"example_proteins":0,"protein_traits_record":false,"records_by_mech":{},"scanned":false},"curation_status":"SEEDED","demotion_reasons":[],"description":"This domain is found in a group of fungal proteins, including Chitin synthase 4 from Gibberella zeae (CHS4), which plays a role in the polymerisation of chitin [[cite:PUB00155063],[cite:PUB00155061]]. This domain is predicted to adopt a globular structure with similarity to Cytochrome b5-like heme/steroid binding domain ([pfam:PF00173]). Despite this global similarity this domain lacks haem coordinating residues and is probably not involved in haem binding.","domain_architectures":62,"interpro_id":"IPR054295","known_evidence_count":null,"matches":2475,"name":"Chitin synthase 4-like domain","partial_evidence_count":null,"pfam_id":"PF22997","proteins":2475,"proteomes":1464,"short_name":"CHS4","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF22997","structures":0,"taxa":3068,"unknown_status":"EX_DUF"},"inputs":{"cross_mech":"cross-mech-duf-examples-2026-10-10","members":"pfam-uniprot-uniref90-2026-10-07","protein_examples":"duf-protein-examples-2026-10-13","worklist":"interpro-pfam-duf-2026-10-10"},"members":[],"metadata":{"curation_status":"SEEDED","local_source":"source/data/families/PF22997.yaml","record":{"characterization_status":"UNSCORED","counters":{"alphafold_models":1582,"domain_architectures":62,"matches":2475,"proteins":2475,"proteomes":1464,"structures":0,"taxa":3068},"curation_status":"SEEDED","description":"This domain is found in a group of fungal proteins, including Chitin synthase 4 from Gibberella zeae (CHS4), which plays a role in the polymerisation of chitin [[cite:PUB00155063],[cite:PUB00155061]]. This domain is predicted to adopt a globular structure with similarity to Cytochrome b5-like heme/steroid binding domain ([pfam:PF00173]). Despite this global similarity this domain lacks haem coordinating residues and is probably not involved in haem binding.","id":"Pfam:PF22997","interpro_id":"InterPro:IPR054295","name":"Chitin synthase 4-like domain","pfam_id":"PF22997","provenance":{"generated_at":"2026-10-10T07:44:21.614711Z","path":"data/worklists/interpro-pfam-duf-2026-10-10.json","sha256":"17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383","snapshot_id":"interpro-pfam-duf-2026-10-10","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/"},"seed_status":"EX_DUF","short_name":"CHS4","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF22997"},"source":{"commit":"c8155abfcf881ad8662936343deb81e604e3a194","path":"data/families/PF22997.yaml","repository":"https://github.com/CultureBotAI/DUFMech","sha256":"8c4a0c9f0aff056a572d56d6ab10524c0556366025a3bfa6cea4f990915bdbc7"}},"protein_examples":{"candidate_count":3,"discovery_scanned":true,"example_count":3,"examples":[{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Pyricularia oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/pfam/chs4-pf22997.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF22997","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/pfam/chs4-pf22997.yaml"}],"pfam_ids":["PF03142","PF22997"],"protein_name":"Chitin synthase 4","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MSLPERPGAKASYEQRNSYRKSPSRRNRPNDIEASGYYPVTGGQHQRGPSVNSFAETIRSPNSNIESAPLSPSAEQIEHGSDQPFQRKRSLIRPERNRIDRDHPNYHYRKHAAKMNTLPSSTGNDPVLEDVSGATESGPPSGSNSASGSGVREENIPRKSRKASGRETVAEKSDNTRRRVSTRNSKIVKEGKRKEKIPEQLRPPSAWNVYCAVITFWSPDFIMKCCGMPAKAQRRAWREKIGLISLILIIMGVVGFLTFGFNQAVCGGPVLRLHINSVDRSYMIFHGTAYNLDGSHHPVAEGIPKRLDGTGANVVYDLPEGYGGTDGSFMFQNVNGKCKGLITKAPNSDVPSEGDNLAWYFPCHARNQDGSSQPNMTYPYYFGYACHTTPLARSTFYTQLDKSADVYFTWADIRNNSRNLFVYSGNVLDLDLLFWFNRDQVNIPRRFEELRDKNNAANRAIRGRDATRTFMASGDRQIAECFEDIIKVGTVDTDTVGCIAAKVVLYVSLALILSVVGARFTLALIFQWFISKNYAADKTSQTSDKRKRNKQIEDWSEDIYRAPPRMPGDVGSSVAGASSSDHTSKRSSFLPTTSRFSTVYGAERSARNKSMPTTMASQASGGYMGPSSTAYRETNESRTSFLKSDPYATNTAPIEGPGPSGFIHDSVVPQPPSDWMPFGFPLAHTICLVTAYSEGELGLRTTLDSVAMTDYPNSHKVILVICDGIIKGKGESKSTPEYVLDMMKDHTIPVEDVEAFSYVAVASGSKRHNMAKIYAGFYDYGTQSNIPLDKQQRVPMMVVVKCGTPDEMVKSKPGNRGKRDSQIILMSFLQKVMFDERMTELEYEMFNGLWKVTGISPDFYEIVLMVDADTKVFPDSLTHMISAMVKDPEIMGLCGETKIANKRDSWVSAIQVFEYFISHHLAKSFESVFGGVTCLPGCFCMYRIKAPKGAQNYWVPILANPDVVEHYSENVVDTLHKKNLLLLGEDRYLTTLMLRTFPKRKQVFVPQAVCKTTVPDSFMVLLSQRRRWINSTIHNLMELVLVRDLCGTFCFSMQFVIFIELIGTLVLPAAIAFTFYVVIISIINQPPQIIPLVLLGLILGLPAILIIITAHSWSYVLWMLIYLLSLPVWNFVLPAYAFWKFDDFSWGDTRKTAGEKTKKAGIEYEGEFDSSKITMKRWAEFERDRRARQSQYWGSRENVTGGVRTASGVWASAPPHHHQQQYDEYYSDA","sequence_length":1229,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"aa2914abcf706be2bf4c39fd2d312f74af147e1bb8b36f56923e1047f8d580cb","sequence_version":2,"source_url":"https://rest.uniprot.org/uniprotkb/O13353.json","taxon_id":"242507","uniprot_accession":"O13353","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Saccharomyces cerevisiae (strain ATCC 204508 / S288c)","origins":[{"cited_uniprot_accession":"P29465","commit":"e1c66917e753105c4bf5b7b026193fcefa2b000f","link_basis":["uniprot_pfam_xref"],"mech":"PathwayMech","path":"data/pathways/chitin-biosynthesis.yaml","qualification_status":"NOT_ASSESSED","record_id":"gomodel:YeastPathways_PWY3O-15","section":"uniprot_accession","url":"https://github.com/CultureBotAI/PathwayMech/blob/e1c66917e753105c4bf5b7b026193fcefa2b000f/data/pathways/chitin-biosynthesis.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/localization/go/cell-septum-go0030428.yaml","qualification_status":"NOT_ASSESSED","record_id":"GO:0030428","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/localization/go/cell-septum-go0030428.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/localization/go/chitosome-go0045009.yaml","qualification_status":"NOT_ASSESSED","record_id":"GO:0045009","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/localization/go/chitosome-go0045009.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/molecular_function/go/chitin-synthase-activity-go0004100.yaml","qualification_status":"NOT_ASSESSED","record_id":"GO:0004100","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/molecular_function/go/chitin-synthase-activity-go0004100.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/pathway/go/ascospore-wall-assembly-go0030476.yaml","qualification_status":"NOT_ASSESSED","record_id":"GO:0030476","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/pathway/go/ascospore-wall-assembly-go0030476.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/pathway/go/chitin-biosynthetic-process-go0006031.yaml","qualification_status":"NOT_ASSESSED","record_id":"GO:0006031","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/pathway/go/chitin-biosynthetic-process-go0006031.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/function/pathway/go/protein-localization-to-bud-neck-go0097271.yaml","qualification_status":"NOT_ASSESSED","record_id":"GO:0097271","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/function/pathway/go/protein-localization-to-bud-neck-go0097271.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/cdd/c-terminal-domain-of-chitin-synthase-catalyzes-the-incorporation-of-gl-cd04190.yaml","qualification_status":"NOT_ASSESSED","record_id":"CDD:cd04190","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/cdd/c-terminal-domain-of-chitin-synthase-catalyzes-the-incorporation-of-gl-cd04190.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/interpro/chitin-synthase-4-like-domain-ipr054295.yaml","qualification_status":"NOT_ASSESSED","record_id":"InterPro:IPR054295","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/interpro/chitin-synthase-4-like-domain-ipr054295.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/pfam/chs4-pf22997.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF22997","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/pfam/chs4-pf22997.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/family/pfam/chitin-synth-2-pf03142.yaml","qualification_status":"NOT_ASSESSED","record_id":"Pfam:PF03142","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/family/pfam/chitin-synth-2-pf03142.yaml"}],"pfam_ids":["PF03142","PF22997"],"protein_name":"Chitin synthase 3","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MTGLNGDDPDDYYLNLNQDEESLLRSRHSVGSGAPHRQGSLVRPERSRLNNPDNPHFYYAQKTQEQMNHLDVLPSSTGVNPNATRRSGSLRSKGSVRSKFSGRETDSYLLQDMNTTDKKASVKISDEGVAEDEFDKDGDVDNFEESSTQPINKSIKPLRKETNDTLSFWQMYCYFITFWAPAPILAFCGMPKKERQMAWREKVALISVILYIGAIVAFLTFGFTKTVCSSSKLRLKNNEVSTEFVVINGKAYELDTSSRSGIQDVEVDSDTLYGPWSDAGKDASFLFQNVNGNCHNLITPKSNSSIPHDDDNNLAWYFPCKLKNQDGSSKPNFTVENYAGWNCHTSKEDRDAFYGLKSKADVYFTWDGIKNSSRNLIVYNGDVLDLDLLDWLEKDDVDYPVVFDDLKTSNLQGYDLSLVLSNGHERKIARCLSEIIKVGEVDSKTVGCIASDVVLYVSLVFILSVVIIKFIIACYFRWTVARKQGAYIVDNKTMDKHTNDIEDWSNNIQTKAPLKEVDPHLRPKKYSKKSLGHKRASTFDLLKKHSSKMFQFNESVIDLDTSMSSSLQSSGSYRGMTTMTTQNAWKLSNENKAVHSRNPSTLLPTSSMFWNKATSSPVPGSSLIQSLDSTIIHPDIVQQPPLDFMPYGFPLIHTICFVTCYSEDEEGLRTTLDSLSTTDYPNSHKLLMVVCDGLIKGSGNDKTTPEIALGMMDDFVTPPDEVKPYSYVAVASGSKRHNMAKIYAGFYKYDDSTIPPENQQRVPIITIVKCGTPAEQGAAKPGNRGKRDSQIILMSFLEKITFDERMTQLEFQLLKNIWQITGLMADFYETVLMVDADTKVFPDALTHMVAEMVKDPLIMGLCGETKIANKAQSWVTAIQVFEYYISHHQAKAFESVFGSVTCLPGCFSMYRIKSPKGSDGYWVPVLANPDIVERYSDNVTNTLHKKNLLLLGEDRFLSSLMLKTFPKRKQVFVPKAACKTIAPDKFKVLLSQRRRWINSTVHNLFELVLIRDLCGTFCFSMQFVIGIELIGTMVLPLAICFTIYVIIFAIVSKPTPVITLVLLAIILGLPGLIVVITATRWSYLWWMCVYICALPIWNFVLPSYAYWKFDDFSWGDTRTIAGGNKKAQDENEGEFDHSKIKMRTWREFEREDILNRKEESDSFVA","sequence_length":1165,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"a76969e44f9ba9152a10e46f9e89aa739a002fd91ba45a770a19e6636f3f3265","sequence_version":3,"source_url":"https://rest.uniprot.org/uniprotkb/P29465.json","taxon_id":"559292","uniprot_accession":"P29465","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Exophiala dermatitidis","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/pfam/chs4-pf22997.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF22997","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/pfam/chs4-pf22997.yaml"}],"pfam_ids":["PF03142","PF22997"],"protein_name":"Chitin synthase 4","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MAEPRMPRPAISPTRDKSHSPYRESPSRRLRDVETGYNPPQNPPYPPQRNGTEPYPPSPSSRTADWEPIMSNPNPMSQSDLGRKKSLIRPERNRIDRDHPNYYYRKHAANMEVLPSTTGNDPVVEDLAEHRTVSSGSTQQDTSIEEEVVGNPQKSRMPGKLEPVGKKKAPRKLVRKDTRNLTEEEKRRQKELDKIKPPSIWNIYCAVVTFWAPDCLLQCFGMPARAQRRAWKEKVGLISIILLIAAFVGFLTFGFTQAVCAAPGLRLKINHVDRSYMIFHGGAYNLDGSMHPAARGIPLDANVLYDLPHKYGGQDGSFMFQKVNGACKGLITLAEGSDVPTNSDGDLAWYFPCTAFNQDGSSEVNLTSPYYLGYACHTTAKARNTFYSLKKAGDVYFTWDDIKNKSRNLAVYSGSVVDLDLLKWFNKSQVAWPQQFDDLRENGRVRGMDLTHVLQSATDKQVGRCLTQIAKVGSIDTESVGCIASKVVLYVSLVFILAIVAAKFFLALAFQWFLARRFAAAKTSQTSDPKKRAKQIEDWTDDIYKPAPKITDPASTVTGSDGRTSKRGSMFLPQTSRFTSPYAVDRRSSRPPPTTMTSQSSNAKLLPGGNPYKSGFNSSQNTLDLHSRMSVGASRSSLLLSGQETRYSAVMDNLDPNGPVGFIHENVVPQPPPEWQPFGYPLAHVICLVTAYSEGEDGIRTTLDSIATTDYPNSHKAILVVCDGMIKGKGEAQSTPDIVLGMMGDFVIAPEDVQAFSYVAVSSGAKRHNMAKVYAGFYDYGPKSRIDPTKQQRVPMMVVVKCGTPDEATKSKPGNRGKRDSQIILMSFLQKVMFDERMTELEYEMFNGLWKVTGMSPDFYEMVLMVDADTKVFPDSLTHMVSAMVKDPEIMGLCGETKIANKNASWVSRIQVFEYFISHHLSKSFESVFGGVTCLPGCFCMYRIKSPKGGQNYWVPILANPDIVEHYSENVVDTLHKKNLLLLGEDRYLSTLMLKTFPKRKQVFVPQAVCKTTVPDEFKVLLSQRRRWINSTVHNLMELVLVRDLCGTFCFSMQFVVFIELIGTLVLPAAISFTFYLIILSIVKKPVPVIPLVLLALILGLPAILIVLTAHRWSYILWMGIYLLSLPIWNFVLPAYAFWKFDDFSWGETRKTAGEKTKKAGLEYEGEFDSSKITMKRWGDFERGKLDQRSSVCGILVETNLCHLFRTASSGERKCLEPATSYASTERLRFYAWVRTLP","sequence_length":1238,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"0060f0d48d023030e1467857e868dbe85deba6173bb1c0417a31270ed1f04103","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/Q9Y795.json","taxon_id":"5970","uniprot_accession":"Q9Y795","uniprot_release":"2026_03"}],"pfam_id":"PF22997","short_name":"CHS4","status":"HAS_SEQUENCE_EXAMPLE","unresolved":[]}}