{"cross_mech":[],"family":{"alphafold_models":333,"candidate_reasons":["pfam_previous_unknown_name"],"characterization_status":"","context_evidence_count":null,"cross_mech":{"example_proteins":0,"protein_traits_record":false,"records_by_mech":{},"scanned":false},"curation_status":"SEEDED","demotion_reasons":[],"description":"This domain, previously known as DUF7681, is found in phage proteins including Anti-CBASS protein 2 (Acb2) and Thoeris anti-defense 1 (Tad1). It folds into an alpha-helical hairpin that is found either standalone (in Acb2) or in combination with a four-stranded antiparallel beta-sheet domain (in Tad1) [[cite:PUB00153173],[cite:PUB00156031],[cite:PUB00156032]]. Prokaryotic anti-phage immune systems use TIR and cGAS-like enzymes to produce 1-3-glycocyclic ADP-ribose; and cyclic dinucleotide and trinucleotide signalling molecules, respectively, which limit phage replication. The Thoeris anti-defence proteins Tad1 and Tad2 are both pronged inhibitors and antagonise immunity alongside Anti-CBASS protein 2. These proteins achieve anti-cyclic-oligonucleotide-based anti-phage signalling system (anti-CBASS) activity by sequestering a considerable amount of cyclic nucleotides. Each of these proteins is using distinct binding pockets. The residues conserved in Tad1 and Acb2 are located near these binding sites [[cite:PUB00156032],[cite:PUB00156033]].","domain_architectures":7,"interpro_id":"IPR056098","known_evidence_count":null,"matches":832,"name":"Acb2/Tad1, hairpin domain","partial_evidence_count":null,"pfam_id":"PF24729","proteins":831,"proteomes":784,"short_name":"Acb2_Tad1_hairpin","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF24729","structures":20,"taxa":1869,"unknown_status":"EX_DUF"},"inputs":{"cross_mech":"cross-mech-duf-examples-2026-10-10","members":"pfam-uniprot-uniref90-2026-10-07","protein_examples":"duf-protein-examples-2026-10-13","worklist":"interpro-pfam-duf-2026-10-10"},"members":[],"metadata":{"curation_status":"SEEDED","local_source":"source/data/families/PF24729.yaml","record":{"characterization_status":"UNSCORED","counters":{"alphafold_models":333,"domain_architectures":7,"matches":832,"proteins":831,"proteomes":784,"structures":20,"taxa":1869},"curation_status":"SEEDED","description":"This domain, previously known as DUF7681, is found in phage proteins including Anti-CBASS protein 2 (Acb2) and Thoeris anti-defense 1 (Tad1). It folds into an alpha-helical hairpin that is found either standalone (in Acb2) or in combination with a four-stranded antiparallel beta-sheet domain (in Tad1) [[cite:PUB00153173],[cite:PUB00156031],[cite:PUB00156032]]. Prokaryotic anti-phage immune systems use TIR and cGAS-like enzymes to produce 1-3-glycocyclic ADP-ribose; and cyclic dinucleotide and trinucleotide signalling molecules, respectively, which limit phage replication. The Thoeris anti-defence proteins Tad1 and Tad2 are both pronged inhibitors and antagonise immunity alongside Anti-CBASS protein 2. These proteins achieve anti-cyclic-oligonucleotide-based anti-phage signalling system (anti-CBASS) activity by sequestering a considerable amount of cyclic nucleotides. Each of these proteins is using distinct binding pockets. The residues conserved in Tad1 and Acb2 are located near these binding sites [[cite:PUB00156032],[cite:PUB00156033]].","id":"Pfam:PF24729","interpro_id":"InterPro:IPR056098","name":"Acb2/Tad1, hairpin domain","pfam_id":"PF24729","provenance":{"generated_at":"2026-10-10T07:44:21.614711Z","path":"data/worklists/interpro-pfam-duf-2026-10-10.json","sha256":"17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383","snapshot_id":"interpro-pfam-duf-2026-10-10","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/"},"seed_status":"EX_DUF","short_name":"Acb2_Tad1_hairpin","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF24729"},"source":{"commit":"c8155abfcf881ad8662936343deb81e604e3a194","path":"data/families/PF24729.yaml","repository":"https://github.com/CultureBotAI/DUFMech","sha256":"aa81a419393c067af3ced3617705560f29a7d9d185926211b635c44b56f2eab4"}},"protein_examples":{"candidate_count":3,"discovery_scanned":true,"example_count":3,"examples":[{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Pseudomonas phage PaMx33","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/pfam/acb2-tad1-hairpin-pf24729.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF24729","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/pfam/acb2-tad1-hairpin-pf24729.yaml"}],"pfam_ids":["PF24729"],"protein_name":"Anti-CBASS protein 2","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MDNQHKKIKGYRDLSQEEIDMMNRVKELGSQFEKLIQDVSDHLRGQYNASLHNRDEITRIANAEPGRWLAIGKTDIQTGMMAIIRAIAQPDSF","sequence_length":93,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"3655b662fe2105e25f1806d54ce81c753e404b59ec137ba605e07b8662afd54a","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/A0A1C8HPQ3.json","taxon_id":"1815974","uniprot_accession":"A0A1C8HPQ3","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Clostridium botulinum","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/pfam/acb2-tad1-hairpin-pf24729.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF24729","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/pfam/acb2-tad1-hairpin-pf24729.yaml"}],"pfam_ids":["PF24729"],"protein_name":"Thoeris anti-defense 1","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MKELSTIQKREKLNTVERIGSEGPGGAYHEYVIKSNSMDSQGNYDVYETIKFQKGARKEEKSQHGVIDSDLLEIVRDRLKSFQAGPFSSRENACALTHVEEALMWMNRRVEDRIERNVLGTNTK","sequence_length":124,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"bd548685fc180110878febe2e493f78e2fa4bb7e7b50626559b9c933475f6c92","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/P0DW58.json","taxon_id":"1491","uniprot_accession":"P0DW58","uniprot_release":"2026_03"},{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Enterobacteria phage T4","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["canonical_example_family_classification","ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/pfam/acb2-tad1-hairpin-pf24729.yaml","qualification_status":"LEGACY_OR_UNSPECIFIED","record_id":"Pfam:PF24729","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/pfam/acb2-tad1-hairpin-pf24729.yaml"}],"pfam_ids":["PF24729"],"protein_name":"Anti-CBASS protein 2","registry_status":"NOT_IN_REGISTRY","reviewed":true,"sequence":"MIEDIKGYKPHTEEKIGKVNAIKDAEVRLGLIFDALYDEFWEALDNCEDCEFAKNYAESLDQLTIAKTKLKEASMWACRAVFQPEEKY","sequence_length":88,"sequence_origin":"UNIPROT_FETCH","sequence_sha256":"ce3c8e63d7254a6a4211458b3ecd4ae8b0a3b57cc11023a3a26e6ab61e12d82d","sequence_version":1,"source_url":"https://rest.uniprot.org/uniprotkb/P13314.json","taxon_id":"10665","uniprot_accession":"P13314","uniprot_release":"2026_03"}],"pfam_id":"PF24729","short_name":"Acb2_Tad1_hairpin","status":"HAS_SEQUENCE_EXAMPLE","unresolved":[]}}