{"cross_mech":[{"cited_uniprot_accession":"","family_mentioned_in_record":null,"link_basis":["trait_identifier"],"pfam_id":"PF30400","protein_label":"","reviewed":null,"short_name":"DUF8616","source_category":"sequence/domain/interpro","source_mech":"ProteinTraitsMech","source_path":"data/traits/sequence/domain/interpro/domain-of-unknown-function-duf8616-ipr061677.yaml","source_record_id":"InterPro:IPR061677","source_record_label":"Domain of unknown function DUF8616","source_section":"trait_identifier","taxon_id":"","taxon_label":"","uniprot_accession":"","unknown_status":"UNKNOWN_CANDIDATE"},{"cited_uniprot_accession":"","family_mentioned_in_record":null,"link_basis":["trait_identifier"],"pfam_id":"PF30400","protein_label":"","reviewed":null,"short_name":"DUF8616","source_category":"sequence/domain/pfam","source_mech":"ProteinTraitsMech","source_path":"data/traits/sequence/domain/pfam/duf8616-pf30400.yaml","source_record_id":"Pfam:PF30400","source_record_label":"Domain of unknown function (DUF8616)","source_section":"trait_identifier","taxon_id":"","taxon_label":"","uniprot_accession":"","unknown_status":"UNKNOWN_CANDIDATE"}],"family":{"alphafold_models":215,"candidate_reasons":["short_name_matches_duf","name_says_unknown_function","domain_of_unknown_function"],"characterization_status":"","context_evidence_count":null,"cross_mech":{"example_proteins":0,"protein_traits_record":true,"records_by_mech":{},"scanned":true},"curation_status":"SEEDED","demotion_reasons":[],"description":"This entry represents a C-terminal domain found in RHS (rearrangement hotspot) family proteins and related polymorphic toxin proteins. It is often associated with ADP-ribosyltransferase toxin domains ([pfam:PF03496]) and RHS repeats ([pfam:PF03527]). In Escherichia coli K12, the domain is found in YibJ, which may be a pseudogene. The family is predominantly found in Actinomycetota, particularly Pseudonocardiaceae, and also in Proteobacteria. The function of this domain remains to be determined. Shows similarity to SteB family [pfam:PF29776] and [pfam:PF14252].","domain_architectures":185,"interpro_id":"IPR061677","known_evidence_count":null,"matches":968,"name":"Domain of unknown function (DUF8616)","partial_evidence_count":null,"pfam_id":"PF30400","proteins":819,"proteomes":632,"short_name":"DUF8616","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF30400","structures":0,"taxa":1086,"unknown_status":"UNKNOWN_CANDIDATE"},"inputs":{"cross_mech":"cross-mech-duf-examples-2026-10-10","members":"pfam-uniprot-uniref90-2026-10-07","protein_examples":"duf-protein-examples-2026-10-13","worklist":"interpro-pfam-duf-2026-10-10"},"members":[],"metadata":{"curation_status":"SEEDED","local_source":"source/data/families/PF30400.yaml","record":{"characterization_status":"UNSCORED","counters":{"alphafold_models":215,"domain_architectures":185,"matches":968,"proteins":819,"proteomes":632,"structures":0,"taxa":1086},"curation_status":"SEEDED","description":"This entry represents a C-terminal domain found in RHS (rearrangement hotspot) family proteins and related polymorphic toxin proteins. It is often associated with ADP-ribosyltransferase toxin domains ([pfam:PF03496]) and RHS repeats ([pfam:PF03527]). In Escherichia coli K12, the domain is found in YibJ, which may be a pseudogene. The family is predominantly found in Actinomycetota, particularly Pseudonocardiaceae, and also in Proteobacteria. The function of this domain remains to be determined. Shows similarity to SteB family [pfam:PF29776] and [pfam:PF14252].","id":"Pfam:PF30400","interpro_id":"InterPro:IPR061677","name":"Domain of unknown function (DUF8616)","pfam_id":"PF30400","provenance":{"generated_at":"2026-10-10T07:44:21.614711Z","path":"data/worklists/interpro-pfam-duf-2026-10-10.json","sha256":"17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383","snapshot_id":"interpro-pfam-duf-2026-10-10","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/"},"seed_status":"UNKNOWN_CANDIDATE","short_name":"DUF8616","source_url":"https://www.ebi.ac.uk/interpro/api/entry/pfam/PF30400"},"source":{"commit":"c8155abfcf881ad8662936343deb81e604e3a194","path":"data/families/PF30400.yaml","repository":"https://github.com/CultureBotAI/DUFMech","sha256":"78dbf6176af9ade1be2f896c9bab880293392ed53974be117d1219d751413d76"}},"protein_examples":{"candidate_count":1,"discovery_scanned":true,"example_count":1,"examples":[{"evidence_scope":"FAMILY_MEMBERSHIP_NOT_FUNCTION","organism":"Escherichia coli (strain K12)","origins":[{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["ptm_own_pfam_record_example"],"mech":"ProteinTraitsMech","path":"data/traits/sequence/domain/pfam/duf8616-pf30400.yaml","qualification_status":"QUALIFIED","record_id":"Pfam:PF30400","section":"canonical_examples","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/traits/sequence/domain/pfam/duf8616-pf30400.yaml"},{"cited_uniprot_accession":"","commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","link_basis":["ptm_registry_exact_uniprot_pfam_xref"],"mech":"ProteinTraitsMech","path":"data/grounding/protein_registry.jsonl","qualification_status":"NOT_ASSESSED_FOR_THIS_FAMILY","record_id":"UniProtKB:P32109","section":"protein_registry","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/grounding/protein_registry.jsonl"}],"pfam_ids":["PF03527","PF30400"],"protein_name":"Putative uncharacterized protein YibJ","registry_source":{"commit":"70edd6f8458845f7d7783ac4f416df519f5e1de6","path":"data/grounding/protein_registry.jsonl","repository":"https://github.com/CultureBotAI/proteintraitsmech","sequence_sha256":"d5e05d2645bae376401ef37e99388db358a6c55e1fac6e12eeb1a8dd1d8d6a8c","sequence_version":3,"sha256":"1be0cf02b76b03a8fe9b80bcead9cd4a419157116eb8da038f266bcc33015eb9","uniprot_release":"2026_03","url":"https://github.com/CultureBotAI/proteintraitsmech/blob/70edd6f8458845f7d7783ac4f416df519f5e1de6/data/grounding/protein_registry.jsonl"},"registry_status":"EXACT_SEQUENCE_VERSION_MATCH","reviewed":true,"sequence":"MQMLDRLESEILADRVSEESRRWLASCGLTVEQMKNQMDPVYTPARKIHLYHCDHRGLPLALISTEGATAWCAEYDEWGNLLSDENPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLLGRYITQDPIGLKGGWNFYQYPLNPVINVDPQGLVDINLYPESDLIHSVADEINIPGVFTIGGHGTPTSIESATRSIMTAKDLAYLIKFDGNYKDGMTVWLFSCNTGKGQNSFAS","sequence_length":233,"sequence_origin":"PTM_REGISTRY_MIRROR","sequence_sha256":"d5e05d2645bae376401ef37e99388db358a6c55e1fac6e12eeb1a8dd1d8d6a8c","sequence_version":3,"source_url":"https://rest.uniprot.org/uniprotkb/P32109.json","taxon_id":"83333","uniprot_accession":"P32109","uniprot_release":"2026_03"}],"pfam_id":"PF30400","short_name":"DUF8616","status":"HAS_SEQUENCE_EXAMPLE","unresolved":[]}}