id: Pfam:PF01207
pfam_id: PF01207
name: Dihydrouridine synthase (Dus)
short_name: Dus
seed_status: EX_DUF
characterization_status: UNSCORED
curation_status: SEEDED
source_url: https://www.ebi.ac.uk/interpro/api/entry/pfam/PF01207
counters:
  proteins: 46157
  matches: 47750
  proteomes: 21293
  taxa: 34157
  structures: 15
  alphafold_models: 33123
  domain_architectures: 370
provenance:
  snapshot_id: interpro-pfam-duf-2026-10-10
  path: data/worklists/interpro-pfam-duf-2026-10-10.json
  sha256: 17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383
  generated_at: '2026-10-10T07:44:21.614711Z'
  source_url: https://www.ebi.ac.uk/interpro/api/entry/pfam/
interpro_id: InterPro:IPR035587
description: Members of this family catalyse the reduction of the 5,6-double bond of a uridine residue
  on tRNA. Dihydrouridine modification of tRNA is widely observed in prokaryotes and eukaryotes, and also
  in some archae. Most dihydrouridines are found in the D loop of t-RNAs. The role of dihydrouridine in
  tRNA is currently unknown, but may increase conformational flexibility of the tRNA. It is likely that
  different family members have different substrate specificities, which may overlap. Dus 1 ([swissprot:Q9HGN6])
  from Saccharomyces cerevisiae acts on pre-tRNA-Phe, while Dus 2 ([swissprot:P53720]) acts on pre-tRNA-Tyr
  and pre-tRNA-Leu. Dus 1 is active as a single subunit, requiring NADPH or NADH, and is stimulated by
  the presence of FAD [[cite:PUB00014304]]. Some family members may be targeted to the mitochondria and
  even have a role in mitochondria [[cite:PUB00014304]].