id: Pfam:PF01980
pfam_id: PF01980
name: tRNA-methyltransferase O N-terminal domain
short_name: TrmO_N
seed_status: EX_DUF
characterization_status: UNSCORED
curation_status: SEEDED
source_url: https://www.ebi.ac.uk/interpro/api/entry/pfam/PF01980
counters:
  proteins: 8639
  matches: 8684
  proteomes: 7015
  taxa: 12908
  structures: 6
  alphafold_models: 5968
  domain_architectures: 76
provenance:
  snapshot_id: interpro-pfam-duf-2026-10-10
  path: data/worklists/interpro-pfam-duf-2026-10-10.json
  sha256: 17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383
  generated_at: '2026-10-10T07:44:21.614711Z'
  source_url: https://www.ebi.ac.uk/interpro/api/entry/pfam/
interpro_id: InterPro:IPR023370
description: This family includes members such as TrmO (tRNA-methyltransferase O) also known as YaeB,
  which contains a single-sheeted beta-barrel structure. TrmO is an AdoMet-dependent methyltransferase
  responsible for m6t6A formation [[cite:PUB00086552]]. Its human homolog, is responsible for formation
  of m6t6A37 in cytoplasmic tRNASer. Lack of TrmO decreases attenuation activity of the thr operon, indicating
  that N6 methylation of m6t6A37 ensures efficient decoding of ACY codons [[cite:PUB00137799]]. In bacteria
  and eukaryotes, TrmO has a C-terminal domain containing the conserved DPRxxY motif. Where the Asp194
  and Arg196 in this motif of E. coli TrmO are necessary for N6-methylation. However, no archaeal YaeB
  has a C-terminal domain containing the DPRxxY motif that is conserved in bacterial and mammalian TrmO
  homologs [[cite:PUB00086552]]. This entry represents the N-terminal domain of TrmO.