id: Pfam:PF03381
pfam_id: PF03381
name: LEM3 (ligand-effect modulator 3) family / CDC50 family
short_name: CDC50
seed_status: EX_DUF
characterization_status: UNSCORED
curation_status: SEEDED
source_url: https://www.ebi.ac.uk/interpro/api/entry/pfam/PF03381
counters:
  proteins: 8587
  matches: 8770
  proteomes: 3431
  taxa: 9045
  structures: 62
  alphafold_models: 7263
  domain_architectures: 147
provenance:
  snapshot_id: interpro-pfam-duf-2026-10-10
  path: data/worklists/interpro-pfam-duf-2026-10-10.json
  sha256: 17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383
  generated_at: '2026-10-10T07:44:21.614711Z'
  source_url: https://www.ebi.ac.uk/interpro/api/entry/pfam/
interpro_id: InterPro:IPR005045
description: Members of this family have been predicted to contain transmembrane helices. The family member
  LEM3 ([swissprot:P42838]) is a ligand-effect modulator, mutation of which increases glucocorticoid receptor
  activity in response to dexamethasone and also confers increased activity on other intracellular receptors
  including the progesterone, oestrogen and mineralocorticoid receptors. LEM3 is thought to affect a downstream
  step in the glucocorticoid receptor pathway. Factors that modulate ligand responsiveness are likely
  to contribute to the context-specific actions of the glucocorticoid receptor in mammalian cells [[cite:PUB00019272]].
  The products of genes YNR048w ([swissprot:P53740]), YNL323w ([swissprot:P42838]) and YCR094w ([swissprot:P25656])
  (CDC50) show redundancy of function and are involved in regulation of transcription via CDC39 [[cite:PUB00019273]].
  CDC39 (also known as NOT1) is normally a negative regulator of transcription either by affecting the
  general RNA polymerase II machinery or by altering chromatin structure [[cite:PUB00019275]]. One function
  of CDC39 is to block activation of the mating response pathway in the absence of pheromone, and mutation
  causes arrest in G1 by activation of the pathway [[cite:PUB00019274]]. It may be that the cold-sensitive
  arrest in G1 noticed in CDC50 mutants [[cite:PUB00019273]] may be due to inactivation of CDC39. The
  effects of LEM3 on glucocorticoid receptor activity may also be due to effects on transcription via
  CDC39.