id: Pfam:PF03652
pfam_id: PF03652
name: Holliday junction resolvase
short_name: RuvX
seed_status: EX_DUF
characterization_status: UNSCORED
curation_status: SEEDED
source_url: https://www.ebi.ac.uk/interpro/api/entry/pfam/PF03652
counters:
  proteins: 19330
  matches: 19371
  proteomes: 17943
  taxa: 26077
  structures: 7
  alphafold_models: 12884
  domain_architectures: 56
provenance:
  snapshot_id: interpro-pfam-duf-2026-10-10
  path: data/worklists/interpro-pfam-duf-2026-10-10.json
  sha256: 17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383
  generated_at: '2026-10-10T07:44:21.614711Z'
  source_url: https://www.ebi.ac.uk/interpro/api/entry/pfam/
interpro_id: InterPro:IPR005227
description: The central intermediate formed during mitotic and meiotic recombination is a four stranded
  DNA structure, also known as the Holliday junction (HJ), and its efficient resolution is essential for
  proper segregation of chromosomes. Resolution of HJs is mediated by a diverse group of DNA structure
  specific endonucleases known as Holliday junction resolvases (HJR) [[cite:PUB00108910]]. This entry
  is specific for RuvX also known as YqgF a family of nucleases which resolves the Holliday junction intermediates
  in genetic recombination[[cite:PUB00009701],[cite:PUB00001215]]. Studies carried out in M. tuberculosis,
  have shown that YqgF/RuvX is a genuine HJR analogous to RuvC from E. coli. Furthermore, a single cysteine
  present in M. tuberculosis RuvX was found to be required for disulfide-bond mediated intermolecular
  dimerization and HJ resolution activity, suggesting that M. tuberculosis RuvX has adapted its YqgF protein
  to function like a typical RuvC family HJR [[cite:PUB00108910]].