id: Pfam:PF03656
pfam_id: PF03656
name: Pam16
short_name: Pam16
seed_status: EX_DUF
characterization_status: UNSCORED
curation_status: SEEDED
source_url: https://www.ebi.ac.uk/interpro/api/entry/pfam/PF03656
counters:
  proteins: 4502
  matches: 4516
  proteomes: 3157
  taxa: 8470
  structures: 1
  alphafold_models: 3869
  domain_architectures: 88
provenance:
  snapshot_id: interpro-pfam-duf-2026-10-10
  path: data/worklists/interpro-pfam-duf-2026-10-10.json
  sha256: 17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383
  generated_at: '2026-10-10T07:44:21.614711Z'
  source_url: https://www.ebi.ac.uk/interpro/api/entry/pfam/
description: The Pam16 protein ([swissprot:P42949]) is the fifth essential subunit of the pre-sequence
  translocase-associated protein import motor (PAM) [[cite:PUB00019986]]. In Saccharomyces cerevisiae,
  Pam16 is required for preprotein translocation into the matrix, but not for protein insertion into the
  inner membrane [[cite:PUB00019986]]. Pam16 has a degenerate J domain. J-domain proteins play important
  regulatory roles as co-chaperones, recruiting Hsp70 partners and accelerating the ATP-hydrolysis step
  of the chaperone cycle [[cite:PUB00005482]]. Pam16's J-like domain strongly interacts with Pam18's J
  domain, leading to a productive interaction of Pam18 with mtHsp70 at the mitochondria import channel
  [[cite:PUB00033671]]. Pam18 stimulates the ATPase activity of mtHsp70.