id: Pfam:PF04167
pfam_id: PF04167
name: Protein of unknown function (DUF402)
short_name: DUF402
seed_status: UNKNOWN_CANDIDATE
characterization_status: UNSCORED
curation_status: SEEDED
source_url: https://www.ebi.ac.uk/interpro/api/entry/pfam/PF04167
counters:
  proteins: 8509
  matches: 8523
  proteomes: 5477
  taxa: 7221
  structures: 12
  alphafold_models: 5048
  domain_architectures: 23
provenance:
  snapshot_id: interpro-pfam-duf-2026-10-10
  path: data/worklists/interpro-pfam-duf-2026-10-10.json
  sha256: 17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383
  generated_at: '2026-10-10T07:44:21.614711Z'
  source_url: https://www.ebi.ac.uk/interpro/api/entry/pfam/
interpro_id: InterPro:IPR007295
description: Family member FomD is a protein encoded in the fosfomycin biosynthesis gene cluster [[cite:PUB00009885],[cite:PUB00100530]],
  which hydrolyses (S)-HPP-CMP to give (S)-HPP and CMP in the presence of Mn2 or Co2 [[cite:PUB00100530]].
  FomD also hydrolyses cytidylyl 2-hydroxyethylphosphonate (HEP-CMP), which is a biosynthetic intermediate
  before C-methylation. FomD structure revealed that it has a beta-barrel fold consisting of a large twisted
  antiparallel beta-sheet, a key feature of DUF402-containing proteins. The function of this domain is
  unknown. It has a Tyr residue which activates a water molecule to promote nucleophilic attack on the
  phosphorus atom of the phosphonate moiety [[cite:PUB00100530]]. This domain has also been found in Ntdp
  (nucleoside tri- and diphosphatase, also known as Sa1684) from Staphylococcus aureus [[cite:PUB00100531]].