id: Pfam:PF04220
pfam_id: PF04220
name: Der GTPase activator (YihI)
short_name: YihI
seed_status: EX_DUF
characterization_status: UNSCORED
curation_status: SEEDED
source_url: https://www.ebi.ac.uk/interpro/api/entry/pfam/PF04220
counters:
  proteins: 1287
  matches: 1287
  proteomes: 1241
  taxa: 1738
  structures: 0
  alphafold_models: 828
  domain_architectures: 1
provenance:
  snapshot_id: interpro-pfam-duf-2026-10-10
  path: data/worklists/interpro-pfam-duf-2026-10-10.json
  sha256: 17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383
  generated_at: '2026-10-10T07:44:21.614711Z'
  source_url: https://www.ebi.ac.uk/interpro/api/entry/pfam/
interpro_id: InterPro:IPR007336
description: YihI activates the GTPase activity of Der, a 50S ribosomal subunit stability factor [[cite:PUB00053968]].
  The stimulation is specific to Der as YihI does not stimulate the GTPase activity of Era or ObgE. The
  interaction of YihI with Der requires only the C-terminal 78 amino acids of YihI [[cite:PUB00053968]].
  A yihI deletion mutant is viable and shows a shorter lag period, but the same post-lag growth rate as
  a wild-type strain. yihI is expressed during the lag period. Overexpression of yihI inhibits cell growth
  and biogenesis of the 50S ribosomal subunit [[cite:PUB00053968]]. YihI is an unusual, highly hydrophilic
  protein with an uneven distribution of charged residues, resulting in an N-terminal region with high
  pI and a C-terminal region with low pI [[cite:PUB00053968]].