id: Pfam:PF05127
pfam_id: PF05127
name: RNA cytidine acetyltransferase NAT10/TcmA, helicase domain
short_name: NAT10_TcmA_helicase
seed_status: EX_DUF
characterization_status: UNSCORED
curation_status: SEEDED
source_url: https://www.ebi.ac.uk/interpro/api/entry/pfam/PF05127
counters:
  proteins: 6449
  matches: 6486
  proteomes: 5368
  taxa: 11846
  structures: 35
  alphafold_models: 5050
  domain_architectures: 137
provenance:
  snapshot_id: interpro-pfam-duf-2026-10-10
  path: data/worklists/interpro-pfam-duf-2026-10-10.json
  sha256: 17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383
  generated_at: '2026-10-10T07:44:21.614711Z'
  source_url: https://www.ebi.ac.uk/interpro/api/entry/pfam/
interpro_id: InterPro:IPR007807
description: This domain contains a P-loop (Walker A) motif, suggesting that it has ATPase activity, and
  a Walker B motif. It si found in RNA cytidine acetyltransferase NAT10 from eukaryotes and tRNA(Met)
  cytidine acetyltransferase TcmA from prokaryotes. NAT10 catalyses the formation of N4-acetylcytidine
  (ac4C) modification on mRNAs, 18S rRNA and tRNAs [[cite:PUB00050413],[cite:PUB00076725],[cite:PUB00158943],[cite:PUB00158942]].
  In tRNA(Met) cytidine acetyltransferase (TmcA) it may function as an RNA helicase motor (driven by ATP
  hydrolysis) which delivers the wobble base to the active centre of the GCN5-related N-acetyltransferase
  (GNAT) domain [[cite:PUB00050413]].