id: Pfam:PF05947
pfam_id: PF05947
name: Type VI secretion system, TssF
short_name: T6SS_TssF
seed_status: EX_DUF
characterization_status: UNSCORED
curation_status: SEEDED
source_url: https://www.ebi.ac.uk/interpro/api/entry/pfam/PF05947
counters:
  proteins: 5516
  matches: 5531
  proteomes: 3423
  taxa: 4977
  structures: 3
  alphafold_models: 3292
  domain_architectures: 19
provenance:
  snapshot_id: interpro-pfam-duf-2026-10-10
  path: data/worklists/interpro-pfam-duf-2026-10-10.json
  sha256: 17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383
  generated_at: '2026-10-10T07:44:21.614711Z'
  source_url: https://www.ebi.ac.uk/interpro/api/entry/pfam/
interpro_id: InterPro:IPR010272
description: This is a family of Gram-negative bacterial proteins that form part of the type VI pathogenicity
  secretion system (T6SS) , including TssF. TssF is homologues to phage tail proteins and is required
  for proper assembly of the Hcp tube (the T6SS inner tube) in bacteria [[cite:PUB00077772]]. T6SSs are
  toxin delivery systems. It is a multiprotein complex requiring numerous core proteins (Tss proteins)
  including cytoplasmic, transmembrane, and outer membrane components. The needle or tube apparatus is
  comprised of a phage-like complex, similar to the T4 contractile bacteriophage tail, which is thought
  to be anchored to the membrane by a trans-envelope complex [[cite:PUB00090998]]. T6SSs contain 13 conserved
  components (TssA-TssM) which are thought to form the core apparatus and to enable effector proteins
  to be injected into target cells in a single cell-contact-dependent step. TssF one of the core components
  of T6SSs machinery, has been shown to be recruited by TssK into the membrane-associated T6SS complex,
  contributing to the dynamic mechanism of the system [[cite:PUB00136430]]. Furthermore, it has been reported
  to interact with TssG proteins stabilizing each other while making contact with TssE, TssK and VgrG
  as well as with tube and sheath components. Bioinformatic analysis suggest that TssF and TssG share
  similarities with the J and I proteins of the bacteriophage P2 baseplate respectively. Further experimental
  studies show that functional TssF and TssK proteins assemble into static foci near the cell envelope.
  Moreover, biochemical and cytological approaches provide support to the role of TssE, TssF, TssG, TssK
  and VgrG as T6SS baseplate components and to a sequential recruitment hierarchy (membrane complex, baseplate,
  tail tube/sheath) during T6SS biogenesis [[cite:PUB00077772]].