id: Pfam:PF06581
pfam_id: PF06581
name: Mad1 and Cdc20-bound-Mad2 binding
short_name: p31comet
seed_status: EX_DUF
characterization_status: UNSCORED
curation_status: SEEDED
source_url: https://www.ebi.ac.uk/interpro/api/entry/pfam/PF06581
counters:
  proteins: 1184
  matches: 1201
  proteomes: 998
  taxa: 3345
  structures: 2
  alphafold_models: 1041
  domain_architectures: 7
provenance:
  snapshot_id: interpro-pfam-duf-2026-10-10
  path: data/worklists/interpro-pfam-duf-2026-10-10.json
  sha256: 17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383
  generated_at: '2026-10-10T07:44:21.614711Z'
  source_url: https://www.ebi.ac.uk/interpro/api/entry/pfam/
interpro_id: InterPro:IPR009511
description: This family is involved in the cell-cycle surveillance mechanism called the spindle checkpoint.
  This mechanism monitors the proper bipolar attachment of sister chromatids to spindle microtubules and
  ensures the fidelity of chromosome segregation during mitosis. A key player in mitosis is Mad2, and
  Mad2 exhibits an unusual two-state behaviour. A Mad1-Mad2 core complex recruits cytosolic Mad2 to kinetochores
  through Mad2 dimerisation and converts Mad2 to a conformer amenable to Cdc20 binding. p31comet inactivates
  the checkpoint by binding to Mad1- or Cdc20-bound Mad2 in such a way as to stop Mad2 activation and
  to promote the dissociation of the Mad2-Cdc20 complex [[cite:PUB00049186]].