id: Pfam:PF07792
pfam_id: PF07792
name: Docking domain of Afi1 for Arf3 in vesicle trafficking
short_name: Afi1
seed_status: EX_DUF
characterization_status: UNSCORED
curation_status: SEEDED
source_url: https://www.ebi.ac.uk/interpro/api/entry/pfam/PF07792
counters:
  proteins: 1569
  matches: 1570
  proteomes: 1504
  taxa: 3386
  structures: 0
  alphafold_models: 1306
  domain_architectures: 27
provenance:
  snapshot_id: interpro-pfam-duf-2026-10-10
  path: data/worklists/interpro-pfam-duf-2026-10-10.json
  sha256: 17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383
  generated_at: '2026-10-10T07:44:21.614711Z'
  source_url: https://www.ebi.ac.uk/interpro/api/entry/pfam/
interpro_id: InterPro:IPR012860
description: This domain occurs at the N-terminal of Afi1, an Arf3p-interacting protein, is a protein
  necessary for vesicle trafficking in yeast. This domain is the interacting region of the protein which
  binds to Arf3, the highly conserved small GTPases (ADP-ribosylation factors). Afi1 is distributed asymmetrically
  at the plasma membrane and is required for polarized distribution of Arf3 but not of an Arf3 guanine
  nucleotide-exchange factor, Yel1p. However, Afi1 is not required for targeting of Arf3 or Yel1p to the
  plasma membrane. Afi1 functions as an Arf3 polarization-specific adapter and participates in development
  of polarity. Although Arf3 is the homologue of human Arf6 it does not function in the same way, not
  being necessary for endocytosis or for mating factor receptor internalization. In the S phase, however,
  it is concentrated at the plasma membrane of the emerging bud. Because of its polarized localisation
  and its critical function in the normal budding pattern of yeast, Arf3 is probably a regulator of vesicle
  trafficking, which is important for polarized growth.