id: Pfam:PF10288
pfam_id: PF10288
name: Cytoplasmic tRNA 2-thiolation protein 2
short_name: CTU2
seed_status: EX_DUF
characterization_status: UNSCORED
curation_status: SEEDED
source_url: https://www.ebi.ac.uk/interpro/api/entry/pfam/PF10288
counters:
  proteins: 2997
  matches: 2997
  proteomes: 2511
  taxa: 6765
  structures: 0
  alphafold_models: 2543
  domain_architectures: 39
provenance:
  snapshot_id: interpro-pfam-duf-2026-10-10
  path: data/worklists/interpro-pfam-duf-2026-10-10.json
  sha256: 17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383
  generated_at: '2026-10-10T07:44:21.614711Z'
  source_url: https://www.ebi.ac.uk/interpro/api/entry/pfam/
interpro_id: InterPro:IPR019407
description: CTU2 is a family of proteins necessary for the formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine
  in Saccharomyces cerevisiae. The family is conserved from plants to humans ]1]. It plays a central role
  in the 2-thiolation of 5-methoxycarbonylmethyl-2-thiouridine, or the wobble nucleoside [[cite:PUB00109223]].
  This wobble modification in tRNAs, 5-methoxycarbonylmethyl-2-thiouridine (mcm(5)s(2)U), is required
  for the proper decoding of NNR codons in eukaryotes. The 2-thio group gives rigidity by largely fixing
  the C3'-endo ribose puckering, ensuring stable and accurate codon-anticodon pairing [[cite:PUB00069587]].