id: Pfam:PF13901
pfam_id: PF13901
name: Rubicon Homology Domain
short_name: RH_dom
seed_status: EX_DUF
characterization_status: UNSCORED
curation_status: SEEDED
source_url: https://www.ebi.ac.uk/interpro/api/entry/pfam/PF13901
counters:
  proteins: 8015
  matches: 8152
  proteomes: 1750
  taxa: 5484
  structures: 1
  alphafold_models: 6861
  domain_architectures: 123
provenance:
  snapshot_id: interpro-pfam-duf-2026-10-10
  path: data/worklists/interpro-pfam-duf-2026-10-10.json
  sha256: 17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383
  generated_at: '2026-10-10T07:44:21.614711Z'
  source_url: https://www.ebi.ac.uk/interpro/api/entry/pfam/
interpro_id: InterPro:IPR025258
description: This is the Rubicon homology domain (RH) characterised at the C-terminal of Rubicon, PLEKHM1
  and Pacer, proteins that modulate late steps in autophagy [[cite:PUB00100995],[cite:PUB00100997]]. Rubicon
  (RUBCN) negatively regulates autophagy and endolysosomal trafficking by inhibiting PI3K complex II (PI3KC3-C2),
  which impairs autophagosome maturation process. PLEKHM1 is an adapter protein that regulates Rab7-dependent
  and HOPS complex-dependent fusion events in the endolysosomal system and couples autophagic and the
  endocytic trafficking pathways [[cite:PUB00100999],[cite:PUB00100998]]. On the other hand, Pacer positively
  regulates autophagy, promoting autophagosome maturation by facilitating the biogenesis of phosphatidylinositol
  3-phosphate (PtdIns3P) in late steps of autophagy; it antagonises RUBCN [[cite:PUB00101000]]. This domain
  contains nine conserved cysteines and one conserved histidine, which have been predicted to bind divalent
  zinc cations being required for Rubicon and PLEKHM1 to interact with Rab7.