id: Pfam:PF14130
pfam_id: PF14130
name: Cap4, dsDNA endonuclease domain
short_name: Cap4_nuclease
seed_status: EX_DUF
characterization_status: UNSCORED
curation_status: SEEDED
source_url: https://www.ebi.ac.uk/interpro/api/entry/pfam/PF14130
counters:
  proteins: 634
  matches: 634
  proteomes: 609
  taxa: 1422
  structures: 7
  alphafold_models: 353
  domain_architectures: 22
provenance:
  snapshot_id: interpro-pfam-duf-2026-10-10
  path: data/worklists/interpro-pfam-duf-2026-10-10.json
  sha256: 17fb625876c9326ed284263a47bcd84e0d7844a31c040dc06cd2260c36f1c383
  generated_at: '2026-10-10T07:44:21.614711Z'
  source_url: https://www.ebi.ac.uk/interpro/api/entry/pfam/
interpro_id: InterPro:IPR025382
description: This domain family corresponds to the N-terminal of CD-NTase associated protein 4 (Cap4)
  from bacteria and archaea. CD-NTase-associated protein 4 (Cap4) is a nucleotide second messenger sensor
  that functions is CBASS immunity. This is the effector domain which has dsDNA nuclease activity, sharing
  structural homology with type II restriction endonucleases and it has the putative active-site conserved
  residues required for divalent metal coordination. This domain is inactive as a monomer; after Cap4
  signal recognition through its C-terminal SAVED domain ([pfam:PF18145]), it oligomerises to closely
  locate two nuclease effector domains. This domain does not participates in ligand specificity and it
  has a promiscuous DNA cleavage response [[cite:PUB00094500]].